BBa_K510042
type: ComponentDefinition
persistent identity: BBa K510042
display id: BBa_K510042
version: 1
was generated by: iGEM to SBOL conversion
description: pUC18Sfi-miniTn7BB-Gm-Lux
created: 2011-10-18T11:00:00Z
modified: 2015-05-08T01:12:30Z
mutable provenance: The miniTn7BB-Gm minitransposon was synthesized commercially and pUC18SfiI is a commercial vector.
owned by: james
owned by: myers
mutable description: pUC18R6KT-miniTn7BB-Gm-Lux is a vehicle vector for the minitransposon miniTn7BB-Gm-Lux. This transposon is a part of the miniTn7 BioBrick toolkit, a set of plasmids harboring Tn7 derivatives that can be used for integration of BioBricks in single copy at a conserved target (the attTn7 site), in multiple bacterial genomes.
discontinued: false
dominant: false
experience: experience/None
group u list: _673_
m user id: 0
owner id: 6163
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The BBa_K325909 BioBrick was inserted within the BCS of pUC18Sfi-miniTn7BB-Gm by EcoRI and PstI clonning. In order to construct the pUC18Sfi-miniTn7BB-Gm delivery plasmid, the miniTn7BB-Gm transposon was cleaved from the commercial plasmid pMA (Mr. Gene) with SfiI and ligated to SfiI-digested pUC18Sfi. This strategy removes all multi-cloning restriction sites from pUC18Sfi, except for the flanking SfiI, thus guaranteeing that the unique sites in the transposon are not duplicated. The remove of the multi-cloning restriction sites was verified by analytic digestions.
star: false
creator: David Caballero, Fernando Govantes
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K510000
component: BBa_K325909
sequence annotation: BBa_K510000
sequence annotation: BBa_K325909
sequence: https://synbiohub.org/public/igem/BBa_K510042_sequence/1

BBa_K510000
type: Component
persistent identity: component2159843
display id: component2159843
version: 1
top level: BBa_K510042
definition: BBa_K510000
access: public

BBa_K325909
type: Component
persistent identity: component2159859
display id: component2159859
version: 1
top level: BBa_K510042
definition: BBa_K325909
access: public

BBa_K510000
type: SequenceAnnotation
persistent identity: annotation2159843
display id: annotation2159843
version: 1
top level: BBa_K510042
location: https://synbiohub.org/public/igem/BBa_K510042/annotation2159843/range2159843/1
component: BBa_K510000

https://synbiohub.org/public/igem/BBa_K510042/annotation2159843/range2159843/1
type: Range
persistent identity: range2159843
display id: range2159843
version: 1
top level: BBa_K510042
direction: direction/reverse
start: 1
end: 4388
orientation: inline

BBa_K325909
type: SequenceAnnotation
persistent identity: annotation2159859
display id: annotation2159859
version: 1
top level: BBa_K510042
location: https://synbiohub.org/public/igem/BBa_K510042/annotation2159859/range2159859/1
component: BBa_K325909

https://synbiohub.org/public/igem/BBa_K510042/annotation2159859/range2159859/1
type: Range
persistent identity: range2159859
display id: range2159859
version: 1
top level: BBa_K510042
direction: direction/reverse
start: 4397
end: 12033
orientation: inline

BBa_K325909
type: ComponentDefinition
persistent identity: BBa K325909
display id: BBa_K325909
version: 1
was generated by: iGEM to SBOL conversion
description: Lux Operon (under pBAD) Vibrio Fischeri
created: 2010-10-22T11:00:00Z
modified: 2015-05-08T01:12:03Z
mutable provenance: a
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: G28
discontinued: false
dominant: false
experience: experience/Works
group u list: _446_
m user id: 0
owner id: 7466
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: a
star: false
creator: Theo Sanderson and Will Handley
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: AraC
sequence annotation: PC
sequence annotation: pBAD
sequence annotation: putative rbs region
sequence annotation: luxC
sequence annotation: putative rbs
sequence annotation: luxD
sequence annotation: luxA
sequence annotation: luxB
sequence annotation: luxE
sequence annotation: luxG
sequence: https://synbiohub.org/public/igem/BBa_K325909_sequence/1

AraC
type: SequenceAnnotation
persistent identity: annotation2111897
display id: annotation2111897
version: 1
top level: BBa_K325909
location: https://synbiohub.org/public/igem/BBa_K325909/annotation2111897/range2111897/1
role: SO:0000110
role: feature/misc

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type: Range
persistent identity: range2111897
display id: range2111897
version: 1
top level: BBa_K325909
direction: direction/forward
start: 1
end: 1079
orientation: inline

PC
type: SequenceAnnotation
persistent identity: annotation2112036
display id: annotation2112036
version: 1
top level: BBa_K325909
location: https://synbiohub.org/public/igem/BBa_K325909/annotation2112036/range2112036/1
role: SO:0000167
role: feature/promoter

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type: Range
persistent identity: range2112036
display id: range2112036
version: 1
top level: BBa_K325909
direction: direction/forward
start: 1043
end: 1114
orientation: inline

pBAD
type: SequenceAnnotation
persistent identity: annotation2112038
display id: annotation2112038
version: 1
top level: BBa_K325909
location: https://synbiohub.org/public/igem/BBa_K325909/annotation2112038/range2112038/1
role: SO:0000110
role: feature/misc

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type: Range
persistent identity: range2112038
display id: range2112038
version: 1
top level: BBa_K325909
direction: direction/forward
start: 1080
end: 1210
orientation: inline

putative rbs region
type: SequenceAnnotation
persistent identity: annotation2112060
display id: annotation2112060
version: 1
top level: BBa_K325909
location: https://synbiohub.org/public/igem/BBa_K325909/annotation2112060/range2112060/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K325909/annotation2112060/range2112060/1
type: Range
persistent identity: range2112060
display id: range2112060
version: 1
top level: BBa_K325909
direction: direction/forward
start: 1211
end: 1230
orientation: inline

luxC
type: SequenceAnnotation
persistent identity: annotation2112039
display id: annotation2112039
version: 1
top level: BBa_K325909
location: https://synbiohub.org/public/igem/BBa_K325909/annotation2112039/range2112039/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K325909/annotation2112039/range2112039/1
type: Range
persistent identity: range2112039
display id: range2112039
version: 1
top level: BBa_K325909
direction: direction/forward
start: 1231
end: 2660
orientation: inline

putative rbs
type: SequenceAnnotation
persistent identity: annotation2112061
display id: annotation2112061
version: 1
top level: BBa_K325909
location: https://synbiohub.org/public/igem/BBa_K325909/annotation2112061/range2112061/1
role: feature/rbs
role: SO:0000139

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type: Range
persistent identity: range2112061
display id: range2112061
version: 1
top level: BBa_K325909
direction: direction/forward
start: 2664
end: 2668
orientation: inline

luxD
type: SequenceAnnotation
persistent identity: annotation2112076
display id: annotation2112076
version: 1
top level: BBa_K325909
location: https://synbiohub.org/public/igem/BBa_K325909/annotation2112076/range2112076/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K325909/annotation2112076/range2112076/1
type: Range
persistent identity: range2112076
display id: range2112076
version: 1
top level: BBa_K325909
direction: direction/forward
start: 2675
end: 3578
orientation: inline

putative rbs
type: SequenceAnnotation
persistent identity: annotation2112117
display id: annotation2112117
version: 1
top level: BBa_K325909
location: https://synbiohub.org/public/igem/BBa_K325909/annotation2112117/range2112117/1
role: feature/rbs
role: SO:0000139

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type: Range
persistent identity: range2112117
display id: range2112117
version: 1
top level: BBa_K325909
direction: direction/forward
start: 3616
end: 3621
orientation: inline

luxA
type: SequenceAnnotation
persistent identity: annotation2112056
display id: annotation2112056
version: 1
top level: BBa_K325909
location: https://synbiohub.org/public/igem/BBa_K325909/annotation2112056/range2112056/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K325909/annotation2112056/range2112056/1
type: Range
persistent identity: range2112056
display id: range2112056
version: 1
top level: BBa_K325909
direction: direction/forward
start: 3629
end: 4693
orientation: inline

putative rbs
type: SequenceAnnotation
persistent identity: annotation2112062
display id: annotation2112062
version: 1
top level: BBa_K325909
location: https://synbiohub.org/public/igem/BBa_K325909/annotation2112062/range2112062/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K325909/annotation2112062/range2112062/1
type: Range
persistent identity: range2112062
display id: range2112062
version: 1
top level: BBa_K325909
direction: direction/forward
start: 4719
end: 4724
orientation: inline

luxB
type: SequenceAnnotation
persistent identity: annotation2112057
display id: annotation2112057
version: 1
top level: BBa_K325909
location: https://synbiohub.org/public/igem/BBa_K325909/annotation2112057/range2112057/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K325909/annotation2112057/range2112057/1
type: Range
persistent identity: range2112057
display id: range2112057
version: 1
top level: BBa_K325909
direction: direction/forward
start: 4732
end: 5712
orientation: inline

putative rbs
type: SequenceAnnotation
persistent identity: annotation2112119
display id: annotation2112119
version: 1
top level: BBa_K325909
location: https://synbiohub.org/public/igem/BBa_K325909/annotation2112119/range2112119/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K325909/annotation2112119/range2112119/1
type: Range
persistent identity: range2112119
display id: range2112119
version: 1
top level: BBa_K325909
direction: direction/forward
start: 5775
end: 5781
orientation: inline

luxE
type: SequenceAnnotation
persistent identity: annotation2112058
display id: annotation2112058
version: 1
top level: BBa_K325909
location: https://synbiohub.org/public/igem/BBa_K325909/annotation2112058/range2112058/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K325909/annotation2112058/range2112058/1
type: Range
persistent identity: range2112058
display id: range2112058
version: 1
top level: BBa_K325909
direction: direction/forward
start: 5826
end: 6923
orientation: inline

putative rbs
type: SequenceAnnotation
persistent identity: annotation2112122
display id: annotation2112122
version: 1
top level: BBa_K325909
location: https://synbiohub.org/public/igem/BBa_K325909/annotation2112122/range2112122/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K325909/annotation2112122/range2112122/1
type: Range
persistent identity: range2112122
display id: range2112122
version: 1
top level: BBa_K325909
direction: direction/forward
start: 6915
end: 6919
orientation: inline

luxG
type: SequenceAnnotation
persistent identity: annotation2112059
display id: annotation2112059
version: 1
top level: BBa_K325909
location: https://synbiohub.org/public/igem/BBa_K325909/annotation2112059/range2112059/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K325909/annotation2112059/range2112059/1
type: Range
persistent identity: range2112059
display id: range2112059
version: 1
top level: BBa_K325909
direction: direction/forward
start: 6927
end: 7637
orientation: inline

BBa_K510000
type: ComponentDefinition
persistent identity: BBa K510000
display id: BBa_K510000
version: 1
was generated by: iGEM to SBOL conversion
description: pUC18Sfi-miniTn7BB-Gm
created: 2011-09-15T11:00:00Z
modified: 2015-05-08T01:12:30Z
mutable provenance: The pUC18-SfiI cloning vector was kindly provided by the Microbiology laboratory of Centro Andaluz de Biolog??a del Desarrollo (CABD) in Sevilla. The miniTn7BB-Gm synthetic transposon was designed by the iGEM 2011 team UPO-Sevilla based on the set of miniTn7 derivatives developed by Choi et al. (2005) and commercially synthesized by Mr. Gene (Regensburg, Germany). K.-H. Choi, J. B. Gaynor, K. G. White, C. Lopez, C. M. Bosio, R. R. Karkhoff-Schweizer & H. P. Schweizer (2005). A Tn7-based broad-range bacterial cloning and expression system. Nature Methods, vol.2 NO.6, June 2005, 443.
owned by: james
owned by: myers
mutable description: The miniTn7BB-Gm synthetic minitransposon was digested at the flanking SfiI sites and cloned into SfiI-digested pUC18-Sfi. This results in the elimination of the complete pUC18-SfiI multi-cloning site, except for the duplicated SfiI sites that remain on both sides of the transposon, thus facilitating its transfer to other vectors. This plasmid can be used to keep the mini-Tn7-Gm in E. coli and other enterics, and may be used for transposition into the genomes of non-enteric bacteria, in which it is non-replicative. The structure of the mini-Tn7BB-Gm transposon is shown in the figure below. Tn7R and Tn7L are the right and left ends of Tn7 transposon, respectively, required for recognition of the transposase machinery and transposition. FRT is the Flp recombinase target site for excision of the resistance once the transposon is inserted in the genome of a living organism. The gentamycin resistance cassette (Gm) is flanked by restriction sites in order to facilitate the generation of variants with different antibiotic resistance markers. The BioBrick cloning site (BCS) includes the prefix and suffix restrictions sites used in assembly standard 10, and it is the place where BioBricks are inserted for genome integration. The complete construction is flanked by SfiI restriction sites to facilitate cloning.
discontinued: false
dominant: false
experience: experience/Works
group u list: _673_
m user id: 0
owner id: 6157
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: In order to make this vector compatible with assembly standard 10, several restriction sites were removed from the synthetic sequence.
star: false
creator: David Caballero, Fernando Govantes
type: DnaRegion
role: partType/Plasmid
role: SO:0000155
sequence annotation: Suffix
sequence annotation: E. coli his operon terminator
sequence annotation: VR (BBa_G00101) primer binding site
sequence annotation: Tn7-L end
sequence annotation: SfiI restriction site
sequence annotation: Ampicillin resistance (bla)
sequence annotation: ColEI (pUC18) Replication origin
sequence annotation: Tn7-R end
sequence annotation: double terminator (BBa_B0014)
sequence annotation: Flipase recombination sequence (FRT)
sequence annotation: SphI restriction site
sequence annotation: NcoI restrictino site
sequence annotation: Gentamycin resistance
sequence annotation: NcoI restriction site
sequence annotation: VF2 (G00100) primer binding site
sequence annotation: Terminator
sequence annotation: Prefix
sequence: https://synbiohub.org/public/igem/BBa_K510000_sequence/1

Suffix
type: SequenceAnnotation
persistent identity: annotation2137411
display id: annotation2137411
version: 1
top level: BBa_K510000
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137411/range2137411/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K510000/annotation2137411/range2137411/1
type: Range
persistent identity: range2137411
display id: range2137411
version: 1
top level: BBa_K510000
direction: direction/forward
start: 2
end: 21
orientation: inline

E. coli his operon terminator
type: SequenceAnnotation
persistent identity: annotation2137412
display id: annotation2137412
version: 1
top level: BBa_K510000
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137412/range2137412/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_K510000/annotation2137412/range2137412/1
type: Range
persistent identity: range2137412
display id: range2137412
version: 1
top level: BBa_K510000
direction: direction/forward
start: 22
end: 93
orientation: inline

VR (BBa_G00101) primer binding site
type: SequenceAnnotation
persistent identity: annotation2137413
display id: annotation2137413
version: 1
top level: BBa_K510000
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137413/range2137413/1
role: feature/primer binding
role: SO:0005850

https://synbiohub.org/public/igem/BBa_K510000/annotation2137413/range2137413/1
type: Range
persistent identity: range2137413
display id: range2137413
version: 1
top level: BBa_K510000
direction: direction/forward
start: 157
end: 176
orientation: inline

Tn7-L end
type: SequenceAnnotation
persistent identity: annotation2137414
display id: annotation2137414
version: 1
top level: BBa_K510000
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137414/range2137414/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K510000/annotation2137414/range2137414/1
type: Range
persistent identity: range2137414
display id: range2137414
version: 1
top level: BBa_K510000
direction: direction/forward
start: 182
end: 347
orientation: inline

SfiI restriction site
type: SequenceAnnotation
persistent identity: annotation2137415
display id: annotation2137415
version: 1
top level: BBa_K510000
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137415/range2137415/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K510000/annotation2137415/range2137415/1
type: Range
persistent identity: range2137415
display id: range2137415
version: 1
top level: BBa_K510000
direction: direction/forward
start: 348
end: 360
orientation: inline

Ampicillin resistance (bla)
type: SequenceAnnotation
persistent identity: annotation2137416
display id: annotation2137416
version: 1
top level: BBa_K510000
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137416/range2137416/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K510000/annotation2137416/range2137416/1
type: Range
persistent identity: range2137416
display id: range2137416
version: 1
top level: BBa_K510000
direction: direction/forward
start: 966
end: 1826
orientation: inline

ColEI (pUC18) Replication origin
type: SequenceAnnotation
persistent identity: annotation2137417
display id: annotation2137417
version: 1
top level: BBa_K510000
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137417/range2137417/1
role: feature/dna
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K510000/annotation2137417/range2137417/1
type: Range
persistent identity: range2137417
display id: range2137417
version: 1
top level: BBa_K510000
direction: direction/forward
start: 1965
end: 2644
orientation: inline

SfiI restriction site
type: SequenceAnnotation
persistent identity: annotation2137418
display id: annotation2137418
version: 1
top level: BBa_K510000
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137418/range2137418/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K510000/annotation2137418/range2137418/1
type: Range
persistent identity: range2137418
display id: range2137418
version: 1
top level: BBa_K510000
direction: direction/forward
start: 3002
end: 3014
orientation: inline

Tn7-R end
type: SequenceAnnotation
persistent identity: annotation2137419
display id: annotation2137419
version: 1
top level: BBa_K510000
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137419/range2137419/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K510000/annotation2137419/range2137419/1
type: Range
persistent identity: range2137419
display id: range2137419
version: 1
top level: BBa_K510000
direction: direction/forward
start: 3014
end: 3213
orientation: inline

double terminator (BBa_B0014)
type: SequenceAnnotation
persistent identity: annotation2137424
display id: annotation2137424
version: 1
top level: BBa_K510000
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137424/range2137424/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K510000/annotation2137424/range2137424/1
type: Range
persistent identity: range2137424
display id: range2137424
version: 1
top level: BBa_K510000
direction: direction/forward
start: 3224
end: 3318
orientation: inline

Flipase recombination sequence (FRT)
type: SequenceAnnotation
persistent identity: annotation2137420
display id: annotation2137420
version: 1
top level: BBa_K510000
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137420/range2137420/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K510000/annotation2137420/range2137420/1
type: Range
persistent identity: range2137420
display id: range2137420
version: 1
top level: BBa_K510000
direction: direction/forward
start: 3329
end: 3376
orientation: inline

SphI restriction site
type: SequenceAnnotation
persistent identity: annotation2137425
display id: annotation2137425
version: 1
top level: BBa_K510000
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137425/range2137425/1
role: SO:0000110
role: feature/dna

https://synbiohub.org/public/igem/BBa_K510000/annotation2137425/range2137425/1
type: Range
persistent identity: range2137425
display id: range2137425
version: 1
top level: BBa_K510000
direction: direction/forward
start: 3381
end: 3386
orientation: inline

NcoI restrictino site
type: SequenceAnnotation
persistent identity: annotation2137436
display id: annotation2137436
version: 1
top level: BBa_K510000
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137436/range2137436/1
role: feature/dna
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K510000/annotation2137436/range2137436/1
type: Range
persistent identity: range2137436
display id: range2137436
version: 1
top level: BBa_K510000
direction: direction/forward
start: 3387
end: 3392
orientation: inline

Gentamycin resistance
type: SequenceAnnotation
persistent identity: annotation2137421
display id: annotation2137421
version: 1
top level: BBa_K510000
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137421/range2137421/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K510000/annotation2137421/range2137421/1
type: Range
persistent identity: range2137421
display id: range2137421
version: 1
top level: BBa_K510000
direction: direction/reverse
start: 3393
end: 3926
orientation: inline

NcoI restriction site
type: SequenceAnnotation
persistent identity: annotation2137439
display id: annotation2137439
version: 1
top level: BBa_K510000
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137439/range2137439/1
role: SO:0000110
role: feature/dna

https://synbiohub.org/public/igem/BBa_K510000/annotation2137439/range2137439/1
type: Range
persistent identity: range2137439
display id: range2137439
version: 1
top level: BBa_K510000
direction: direction/forward
start: 4184
end: 4189
orientation: inline

SphI restriction site
type: SequenceAnnotation
persistent identity: annotation2137440
display id: annotation2137440
version: 1
top level: BBa_K510000
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137440/range2137440/1
role: SO:0000110
role: feature/dna

https://synbiohub.org/public/igem/BBa_K510000/annotation2137440/range2137440/1
type: Range
persistent identity: range2137440
display id: range2137440
version: 1
top level: BBa_K510000
direction: direction/forward
start: 4190
end: 4195
orientation: inline

Flipase recombination sequence (FRT)
type: SequenceAnnotation
persistent identity: annotation2137422
display id: annotation2137422
version: 1
top level: BBa_K510000
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137422/range2137422/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K510000/annotation2137422/range2137422/1
type: Range
persistent identity: range2137422
display id: range2137422
version: 1
top level: BBa_K510000
direction: direction/forward
start: 4196
end: 4243
orientation: inline

VF2 (G00100) primer binding site
type: SequenceAnnotation
persistent identity: annotation2137442
display id: annotation2137442
version: 1
top level: BBa_K510000
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137442/range2137442/1
role: feature/primer binding
role: SO:0005850

https://synbiohub.org/public/igem/BBa_K510000/annotation2137442/range2137442/1
type: Range
persistent identity: range2137442
display id: range2137442
version: 1
top level: BBa_K510000
direction: direction/forward
start: 4249
end: 4268
orientation: inline

Terminator
type: SequenceAnnotation
persistent identity: annotation2137449
display id: annotation2137449
version: 1
top level: BBa_K510000
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137449/range2137449/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_K510000/annotation2137449/range2137449/1
type: Range
persistent identity: range2137449
display id: range2137449
version: 1
top level: BBa_K510000
direction: direction/forward
start: 4326
end: 4359
orientation: inline

Prefix
type: SequenceAnnotation
persistent identity: annotation2137423
display id: annotation2137423
version: 1
top level: BBa_K510000
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137423/range2137423/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K510000/annotation2137423/range2137423/1
type: Range
persistent identity: range2137423
display id: range2137423
version: 1
top level: BBa_K510000
direction: direction/forward
start: 4367
end: 4387
orientation: inline

https://synbiohub.org/public/igem/BBa_K510042_sequence/1
type: Sequence
persistent identity: BBa K510042 sequence
display id: BBa_K510042_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactagtagcggccgctgcagtccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaataggccaaccagataagtgaaatctagttccaaactattttgtcatttttaattttcgtattagcttacgacgctacacccagttcccatctattttgtcactcttccctaaataatccttaaaaactccatttccacccctcccagttcccaactattttgtccgcccacaggccgcctaggccgtagcttggcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcatatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagccccgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgagacgaaagggcctcgtgatacgcctatttttataggttaatgtcatgataataatggtttcttagacgtcaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttga…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K325909_sequence/1
type: Sequence
persistent identity: BBa K325909 sequence
display id: BBa_K325909_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagcagggaaaataatgaataaatgtattccaatgataattaatggaatgattcaagattttgataattatgcatataaagaagttaaactaaataatgataatagagtaaaattatctgtcattactgaaagttcagtttcaaaaacattaaatatcaaagatagaattaatctaaatttaaatcagattgtgaattttttatataccgttggtcaacgatggaaaagtgaagaatataatcggcgacgaacctatatccgtgagttaaaaacatatcttggttattctgatgaaatggcaagattagaagcgaattggattgcaatgttattgtgctctaaaagtgctttgtatgacattgttaattatgatttgggctctatacacgtattagatgaatggcttccacgtggtgattgctatgttaaagcacaaccgaaaggtgtttctgttcacttgttagctggtaatgttccattatcaggagtgacatctattttgcgtgctattttaacaaaaaatgagtgcattattaaaacttcgtcttcagatccttttactgcaaacgctttagtttccagttttattgatgttaatgcagaccatccaataaccaaatcaatgtctgttatgtattggccgcatgatgaagatatgactctatctcaaagaataatgaatcatgccgacgtggttattgcttggggtggagacgaggcgattaaatgggcggtaaaatattcaccaccgcatgtcgatattctgaaatttggaccaaagaaaagcttaagt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K510000_sequence/1
type: Sequence
persistent identity: BBa K510000 sequence
display id: BBa_K510000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactagtagcggccgctgcagtccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaataggccaaccagataagtgaaatctagttccaaactattttgtcatttttaattttcgtattagcttacgacgctacacccagttcccatctattttgtcactcttccctaaataatccttaaaaactccatttccacccctcccagttcccaactattttgtccgcccacaggccgcctaggccgtagcttggcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcatatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagccccgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgagacgaaagggcctcgtgatacgcctatttttataggttaatgtcatgataataatggtttcttagacgtcaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttga…
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z