##gff-version 3 ##sequence-region BBa_K510043 1 8329 BBa_K510043 . engineered_region 4397 8329 . + 0 ID=BBa_K177038;Name=BBa_K177038 BBa_K510043 . sequence_feature 6232 7460 . + 0 ID=BBa_K177012;Name=BBa_K177012;Parent=BBa_K177038 BBa_K510043 . promoter 6232 6280 . + 0 ID=BBa_R0051;Name=BBa_R0051;Parent=BBa_K177012 BBa_K510043 . engineered_region 6232 6280 . + 0 ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051 BBa_K510043 . promoter 6269 6274 . + 0 ID=annotation2022;Name=-10;Parent=BBa_R0051 BBa_K510043 . non_covalent_binding_site 6256 6272 . + 0 ID=annotation2024;Name=OR1;Parent=BBa_R0051 BBa_K510043 . promoter 6246 6251 . + 0 ID=annotation2023;Name=-35;Parent=BBa_R0051 BBa_K510043 . non_covalent_binding_site 6232 6248 . + 0 ID=annotation2025;Name=OR2;Parent=BBa_R0051 BBa_K510043 . ribosome_entry_site 6289 6301 . + 0 ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K177012 BBa_K510043 . ribosome_entry_site 6289 6301 . + 0 ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032 BBa_K510043 . engineered_region 6289 6301 . + 0 ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032 BBa_K510043 . ribosome_entry_site 6295 6298 . + 0 ID=annotation1710;Name=RBS;Parent=BBa_B0032 BBa_K510043 . CDS 6308 7435 . + 0 ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_K177012 BBa_K510043 . engineered_tag 7436 7460 . + 0 ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012 BBa_K510043 . engineered_region 6308 7435 . + 0 ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012 BBa_K510043 . tag 7397 7435 . + 0 ID=annotation1722;Name=LVA;Parent=BBa_C0012 BBa_K510043 . CDS 6308 7435 . + 0 ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012 BBa_K510043 . engineered_region 4397 6223 . + 0 ID=BBa_K177034;Name=BBa_K177034;Parent=BBa_K177038 BBa_K510043 . ribosome_entry_site 5346 5360 . + 0 ID=BBa_B0030;Name=BBa_B0030;Parent=BBa_K177034 BBa_K510043 . ribosome_entry_site 5353 5357 . + 0 ID=annotation1702;Name=RBS;Parent=BBa_B0030 BBa_K510043 . engineered_region 5346 5360 . + 0 ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030 BBa_K510043 . ribosome_entry_site 5346 5360 . + 0 ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030 BBa_K510043 . terminator 6183 6223 . + 0 ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K177034 BBa_K510043 . stop_codon 6216 6216 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_K510043 . polyA_site 6210 6223 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_K510043 . stem_loop 6190 6209 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_K510043 . engineered_region 6183 6223 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_K510043 . ribosome_entry_site 4605 4617 . + 0 ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_K177034 BBa_K510043 . ribosome_entry_site 4605 4617 . + 0 ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032 BBa_K510043 . engineered_region 4605 4617 . + 0 ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032 BBa_K510043 . ribosome_entry_site 4611 4614 . + 0 ID=annotation1710;Name=RBS;Parent=BBa_B0032 BBa_K510043 . terminator 6095 6174 . + 0 ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K177034 BBa_K510043 . engineered_region 6095 6174 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 BBa_K510043 . stem_loop 6106 6149 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 BBa_K510043 . CDS 4624 5337 . + 0 ID=BBa_K177050;Name=BBa_K177050;Parent=BBa_K177034 BBa_K510043 . CDS 4624 5337 . + 0 ID=annotation2053383;Name=cIts;Parent=BBa_K177050 BBa_K510043 . promoter 4397 4596 . + 0 ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_K177034 BBa_K510043 . non_covalent_binding_site 4485 4522 . + 0 ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010 BBa_K510043 . non_covalent_binding_site 4562 4596 . + 0 ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010 BBa_K510043 . promoter 4533 4538 . + 0 ID=annotation1961224;Name=-35;Parent=BBa_R0010 BBa_K510043 . engineered_region 4397 4596 . + 0 ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010 BBa_K510043 . promoter 4557 4562 . + 0 ID=annotation1961225;Name=-10;Parent=BBa_R0010 BBa_K510043 . start_codon 4569 4569 . + 0 ID=annotation1961227;Name=start;Parent=BBa_R0010 BBa_K510043 . CDS 4397 4484 . + 0 ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010 BBa_K510043 . CDS 5367 6086 . + 0 ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_K177034 BBa_K510043 . CDS 5367 6086 . + 0 ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040 BBa_K510043 . engineered_region 7469 8329 . + 0 ID=BBa_K094130;Name=BBa_K094130;Parent=BBa_K177038 BBa_K510043 . terminator 8289 8329 . + 0 ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K094130 BBa_K510043 . stop_codon 8322 8322 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_K510043 . polyA_site 8316 8329 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_K510043 . stem_loop 8296 8315 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_K510043 . engineered_region 8289 8329 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_K510043 . terminator 8201 8280 . + 0 ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K094130 BBa_K510043 . engineered_region 8201 8280 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 BBa_K510043 . stem_loop 8212 8255 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 BBa_K510043 . ribosome_entry_site 7469 7480 . + 0 ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K094130 BBa_K510043 . conserved_region 7473 7476 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 BBa_K510043 . CDS 7487 8192 . + 0 ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_K094130 BBa_K510043 . engineered_tag 8168 8192 . + 0 ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010 BBa_K510043 . CDS 7487 8161 . + 0 ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010 BBa_K510043 . plasmid 1 4388 . + 0 ID=BBa_K510000;Name=BBa_K510000 BBa_K510043 . sequence_feature 1965 2644 . + 0 ID=annotation2137417;Name=ColEI (pUC18) Replication origin;Parent=BBa_K510000 BBa_K510043 . engineered_region 4367 4387 . + 0 ID=annotation2137423;Name=Prefix;Parent=BBa_K510000 BBa_K510043 . sequence_feature 348 360 . + 0 ID=annotation2137415;Name=SfiI restriction site;Parent=BBa_K510000 BBa_K510043 . sequence_feature 3381 3386 . + 0 ID=annotation2137425;Name=SphI restriction site;Parent=BBa_K510000 BBa_K510043 . sequence_feature 3002 3014 . + 0 ID=annotation2137418;Name=SfiI restriction site;Parent=BBa_K510000 BBa_K510043 . sequence_feature 182 347 . + 0 ID=annotation2137414;Name=Tn7-L end;Parent=BBa_K510000 BBa_K510043 . sequence_feature 4190 4195 . + 0 ID=annotation2137440;Name=SphI restriction site;Parent=BBa_K510000 BBa_K510043 . sequence_feature 4196 4243 . + 0 ID=annotation2137422;Name=Flipase recombination sequence (FRT);Parent=BBa_K510000 BBa_K510043 . sequence_feature 4184 4189 . + 0 ID=annotation2137439;Name=NcoI restriction site;Parent=BBa_K510000 BBa_K510043 . CDS 966 1826 . + 0 ID=annotation2137416;Name=Ampicillin resistance (bla);Parent=BBa_K510000 BBa_K510043 . sequence_feature 3329 3376 . + 0 ID=annotation2137420;Name=Flipase recombination sequence (FRT);Parent=BBa_K510000 BBa_K510043 . sequence_feature 3387 3392 . + 0 ID=annotation2137436;Name=NcoI restrictino site;Parent=BBa_K510000 BBa_K510043 . stem_loop 4326 4359 . + 0 ID=annotation2137449;Name=Terminator;Parent=BBa_K510000 BBa_K510043 . primer_binding_site 157 176 . + 0 ID=annotation2137413;Name=VR (BBa_G00101) primer binding site;Parent=BBa_K510000 BBa_K510043 . sequence_feature 3014 3213 . + 0 ID=annotation2137419;Name=Tn7-R end;Parent=BBa_K510000 BBa_K510043 . engineered_region 2 21 . + 0 ID=annotation2137411;Name=Suffix;Parent=BBa_K510000 BBa_K510043 . engineered_region 3224 3318 . + 0 ID=annotation2137424;Name=double terminator (BBa_B0014);Parent=BBa_K510000 BBa_K510043 . CDS 3393 3926 . + 0 ID=annotation2137421;Name=Gentamycin resistance;Parent=BBa_K510000 BBa_K510043 . primer_binding_site 4249 4268 . + 0 ID=annotation2137442;Name=VF2 (G00100) primer binding site;Parent=BBa_K510000 BBa_K510043 . stem_loop 22 93 . + 0 ID=annotation2137412;Name=E. coli his operon terminator;Parent=BBa_K510000 >BBa_K510043 tactagtagcggccgctgcagtccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaa gattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagc tcactcaaaggcggtaataggccaaccagataagtgaaatctagttccaaactattttgtcatttttaattttcgtatt agcttacgacgctacacccagttcccatctattttgtcactcttccctaaataatccttaaaaactccatttccacccc tcccagttcccaactattttgtccgcccacaggccgcctaggccgtagcttggcactggccgtcgttttacaacgtcgt gactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaag aggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgcctgatgcggtattttctccttac gcatctgtgcggtatttcacaccgcatatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagccc cgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccg tctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgagacgaaagggcctcgtgatacgcct atttttataggttaatgtcatgataataatggtttcttagacgtcaggtggcacttttcggggaaatgtgcgcggaacc cctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataat attgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgt ttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactg gatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgc tatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgactt ggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataacc atgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaaca tgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccac gatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaatta atagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgata aatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagt tatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaag cattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatct aggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgt agaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgcta ccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagatac caaatactgttcttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctct gctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccg gataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactga gatacctacagcgtgagctttgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcag ggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccac ctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttt tacggttcctggccttttgctggccttttgctcacatgttctttcctgcgttatcccctgattctgtggataaccgtat taccgcctttgagtgagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaa gagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgact ggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgct tccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacaggaaacagctatgaccatgattacgaattg gccgcctaggcctgtgggcggacaataaagtcttaaactgaacaaaatagatctaaactatgacaataaagtcttaaac tagacagaatagttgtaaactgaaatcagtccagttatgctgtgaaaaagcatactggacttttgttatggctaaagca aactcttcattttctgaagtgcaaattgcccgtcgtattaaagaggggcgtggggttcgcttgtcacactggctcacct tcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt gctagatcttgaagtacctattccgaagttcctattctctaaaaagtataggaacttcagaggcatgcccatggttagg tggcggtacttgggtcgatatcaaagtgcatcacttcttcccgtatgcccaactttgtatagagagccactgcgggatc gtcaccgtaatctgcttgcacgtagatcacataagcaccaagcgcgttggcctcatgcttgaggagattgatgagcgcg gtggcaatgccctgcctccggtgctcgccggagactgcgagatcatagatatagatctcactacgcggctgctcaaact tgggcagaacgtaagccgcgagagcgccaacaaccgcttcttggtcgaaggcagcaagcgcgatgaatgtcttactacg gagcaagttcccgaggtaatcggagtccggctgatgttgggagtaggtggctacgtctccgaactcacgaccgaaaaga tcaagagcagcccgcatggatttgacttggtcagggccgagcctacatgtgcgaatgatgcccatacttgagccaccta actttgttttagggcgactgccctgctgcgtaacatcgttgctgctgcgtaacatcgttgctgctccataacatcaaac atcgacccacggcgtaacgcgcttgctgcttggatgcccgaggcatagactgtacaaaaaaacagtcataacaagccat gaaaaccgccactgcgccgttaccaccgctgcgttcggtcaaggttctggaccagttgcgtgagcgcatacgctacttg cattacagtttacgaaccgaacaggcttatgtcaattcgatccattgctgttgacaaagggaatcaggggatcttccat gggcatgcgaagtacctattccgaagttcctattctctaaaaagtataggaacttcagagctgccacctgacgtctaag aaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagc tttcgctaaggatgatttctggaattcgcggccgcttctagagtactagagcaatacgcaaaccgcctctccccgcgcg ttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtg agttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggat aacaatttcacacatactagagtcacacaggaaagtactagatgagcacaaaaaagaaaccattaacacaagagcagct tgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagac aagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgc ttacaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggt tagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgag cttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttg aggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccc tgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagg gatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtgg ggaaagttatcgctagtcagtggcctgaagagacgtttggctgatactagagattaaagaggagaaatactagatgcgt aaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctg tcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgt tccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcat gactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaaga cacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatgg aaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatgga atcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactc caattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacga aaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataa taatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcg gtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtaacaccgtg cgtgttgactattttacctctggcggtgataatggttgctactagagtcacacaggaaagtactagatggtgaatgtga aaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccag ccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaa caactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcg cggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctg taaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgcc attgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtatta ttttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagc gggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccg atagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttc ccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttgg tgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacag gattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagc tgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccga ttcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagta gcttaataactctgatagtgctagtgtagatctctactagagaaagaggagaaatactagatggcttcctccgaagacg ttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaagg tgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggac atcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgt ccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctc cctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaa aaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtc tgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccggg tgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgct gaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaa acgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacact ggctcaccttcgggtgggcctttctgcgtttata