LOCUS BBa_K510045 9074 bp DNA linear 26-Dec-2024 DEFINITION pUC18Sfi-miniTn7BB-Gm-improved_flipflop_(module I) ACCESSION BBa_K510045 VERSION BBa_K510045.1 FEATURES Location/Qualifiers misc_feature 2..21 /label=Suffix stem_loop 22..93 /label=E. coli his operon terminator primer_bind 157..176 /label=VR (BBa_G00101) primer binding site misc_feature 182..347 /label=Tn7-L end misc_feature 348..360 /label=SfiI restriction site CDS 966..1826 /label=Ampicillin resistance (bla) misc_feature 1965..2644 /label=ColEI (pUC18) Replication origin misc_feature 3002..3014 /label=SfiI restriction site misc_feature 3014..3213 /label=Tn7-R end misc_feature 3329..3376 /label=Flipase recombination sequence (FRT) CDS 3393..3926 /label=Gentamycin resistance misc_feature 4196..4243 /label=Flipase recombination sequence (FRT) misc_feature 4367..4387 /label=Prefix misc_feature 3224..3318 /label=double terminator (BBa_B0014) misc_feature 3381..3386 /label=SphI restriction site misc_feature 3387..3392 /label=NcoI restrictino site misc_feature 4184..4189 /label=NcoI restriction site misc_feature 4190..4195 /label=SphI restriction site primer_bind 4249..4268 /label=VF2 (G00100) primer binding site stem_loop 4326..4359 /label=Terminator misc_feature 1..4388 /label=BBa_K510000 misc_feature 4406..4534 /label=Double terminator misc_feature 4535..4540 /label=Restriction Site: AflII misc_feature 4543..4550 /label=Restriction Site: AscI CDS 4551..5315 /label=GFP::Das4 misc_feature 4557..4601 /label=DAS4 RBS 5322..5334 /label=RBS misc_feature 5336..5341 /label=Restriction Site: BamHI CDS 5343..6473 /label=LacI::Das4 misc_feature 5349..5393 /label=DAS4 RBS 6480..6492 /label=RBS promoter 6501..6549 /label=PcI misc_feature 6567..6572 /label=Restriction Site: Sma I promoter 6583..6782 /label=PLac RBS 6791..6803 /label=RBS CDS 6810..7523 /label=cI (Ts) misc_feature 7526..7531 /label=Restriction Site: NheI CDS 7535..8128 /label=RBS+OmpN::SspB misc_feature 7535..7630 /label=RBS+OmpN misc_feature 8131..8136 /label=Restriction Site: SacII misc_feature 8138..8233 /label=RBS+OmpN CDS 8138..8914 /label=RBS+OmpN::RFP misc_feature 8922..8927 /label=Restriction Site: StuI misc_feature 8930..9058 /label=Double terminator misc_feature 4397..9074 /label=BBa_K510019 ORIGIN 1 tactagtagc ggccgctgca gtccggcaaa aaaacgggca aggtgtcacc accctgccct 61 ttttctttaa aaccgaaaag attacttcgc gttatgcagg cttcctcgct cactgactcg 121 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaataggc 181 caaccagata agtgaaatct agttccaaac tattttgtca tttttaattt tcgtattagc 241 ttacgacgct acacccagtt cccatctatt ttgtcactct tccctaaata atccttaaaa 301 actccatttc cacccctccc agttcccaac tattttgtcc gcccacaggc cgcctaggcc 361 gtagcttggc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc 421 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc 481 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt 541 attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa 601 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc 661 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga 721 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg 781 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg 841 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa 901 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga 961 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc 1021 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg 1081 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc 1141 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat 1201 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg 1261 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag 1321 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa 1381 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc 1441 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca 1501 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc 1561 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc 1621 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg 1681 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta 1741 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag 1801 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga 1861 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc 1921 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa 1981 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa 2041 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc 2101 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt 2161 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc 2221 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac 2281 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca 2341 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagctt tgagaaagcg 2401 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag 2461 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt 2521 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat 2581 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc 2641 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt 2701 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag 2761 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca 2821 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga 2881 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt 2941 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgaat 3001 tggccgccta ggcctgtggg cggacaataa agtcttaaac tgaacaaaat agatctaaac 3061 tatgacaata aagtcttaaa ctagacagaa tagttgtaaa ctgaaatcag tccagttatg 3121 ctgtgaaaaa gcatactgga cttttgttat ggctaaagca aactcttcat tttctgaagt 3181 gcaaattgcc cgtcgtatta aagaggggcg tggggttcgc ttgtcacact ggctcacctt 3241 cgggtgggcc tttctgcgtt tatatactag agagagaata taaaaagcca gattattaat 3301 ccggcttttt tattatttgc tagatcttga agtacctatt ccgaagttcc tattctctaa 3361 aaagtatagg aacttcagag gcatgcccat ggttaggtgg cggtacttgg gtcgatatca 3421 aagtgcatca cttcttcccg tatgcccaac tttgtataga gagccactgc gggatcgtca 3481 ccgtaatctg cttgcacgta gatcacataa gcaccaagcg cgttggcctc atgcttgagg 3541 agattgatga gcgcggtggc aatgccctgc ctccggtgct cgccggagac tgcgagatca 3601 tagatataga tctcactacg cggctgctca aacttgggca gaacgtaagc cgcgagagcg 3661 ccaacaaccg cttcttggtc gaaggcagca agcgcgatga atgtcttact acggagcaag 3721 ttcccgaggt aatcggagtc cggctgatgt tgggagtagg tggctacgtc tccgaactca 3781 cgaccgaaaa gatcaagagc agcccgcatg gatttgactt ggtcagggcc gagcctacat 3841 gtgcgaatga tgcccatact tgagccacct aactttgttt tagggcgact gccctgctgc 3901 gtaacatcgt tgctgctgcg taacatcgtt gctgctccat aacatcaaac atcgacccac 3961 ggcgtaacgc gcttgctgct tggatgcccg aggcatagac tgtacaaaaa aacagtcata 4021 acaagccatg aaaaccgcca ctgcgccgtt accaccgctg cgttcggtca aggttctgga 4081 ccagttgcgt gagcgcatac gctacttgca ttacagttta cgaaccgaac aggcttatgt 4141 caattcgatc cattgctgtt gacaaaggga atcaggggat cttccatggg catgcgaagt 4201 acctattccg aagttcctat tctctaaaaa gtataggaac ttcagagctg ccacctgacg 4261 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggcaga 4321 atttcagata aaaaaaatcc ttagctttcg ctaaggatga tttctggaat tcgcggccgc 4381 ttctagagta ctagaggaga tcatttataa acgcagaaag gcccacccga aggtgagcca 4441 gtgtgactct agtagagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct 4501 ttcgactgag cctttcgttt tatttgatgc ctggcttaag taggcgcgcc ctattaagaa 4561 gcatcagcat aattttcaga gtagttctcg tcgtttgctg ctttgtatag ttcatccatg 4621 ccatgtgtaa tcccagcagc tgttacaaac tcaagaagga ccatgtggtc tctcttttcg 4681 ttgggatctt tcgaaagggc agattgtgtg gacaggtaat ggttgtctgg taaaaggaca 4741 gggccatcgc caattggagt attttgttga taatggtctg ctagttgaac gcttccatct 4801 tcaatgttgt gtctaatttt gaagttaact ttgattccat tcttttgttt gtctgccatg 4861 atgtatacat tgtgtgagtt atagttgtat tccaatttgt gtccaagaat gtttccatct 4921 tctttaaaat caataccttt taactcgatt ctattaacaa gggtatcacc ttcaaacttg 4981 acttcagcac gtgtcttgta gttcccgtca tctttgaaaa atatagttct ttcctgtaca 5041 taaccttcag gcatggcact cttgaaaaag tcatgctgtt tcatatgatc tgggtatctc 5101 gcaaagcatt gaacaccata accgaaagta gtgacaagtg ttggccatgg aacaggtagt 5161 tttccagtag tgcaaataaa tttaagggta agttttccgt atgttgcatc accttcaccc 5221 tctccactga cagaaaattt gtgcccatta acatcaccat ctaattcaac aagaattggg 5281 acaactccag tgaaaagttc ttctccttta ctcatctagt actttcctgt gtgacggatc 5341 cactatcaag aagcatcagc ataattttca gagtagttct cgtcgtttgc tgcctgcccg 5401 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga 5461 gaggcggttt gcgtattggg cgccagggtg gtttttcttt tcaccagtga gacgggcaac 5521 agctgattgc ccttcaccgc ctggccctga gagagttgca gcaagcggtc cacgctggtt 5581 tgccccagca ggcgaaaatc ctgtttgatg gtggttaacg gcgggatata acatgagctg 5641 tcttcggtat cgtcgtatcc cactaccgag atatccgcac caacgcgcag cccggactcg 5701 gtaatggcgc gcattgcgcc cagcgccatc tgatcgttgg caaccagcat cgcagtggga 5761 acgatgccct cattcagcat ttgcatggtt tgttgaaaac cggacatggc actccagtcg 5821 ccttcccgtt ccgctatcgg ctgaatttga ttgcgagtga gatatttatg ccagccagcc 5881 agacgcagac gcgccgagac agaacttaat gggcccgcta acagcgcgat ttgctggtga 5941 cccaatgcga ccagatgctc cacgcccagt cgcgtaccgt cttcatggga gaaaataata 6001 ctgttgatgg gtgtctggtc agagacatca agaaataacg ccggaacatt agtgcaggca 6061 gcttccacag caatggcatc ctggtcatcc agcggatagt taatgatcag cccactgacg 6121 cgttgcgcga gaagattgtg caccgccgct ttacaggctt cgacgccgct tcgttctacc 6181 atcgacacca ccacgctggc acccagttga tcggcgcgag atttaatcgc cgcgacaatt 6241 tgcgacggcg cgtgcagggc cagactggag gtggcaacgc caatcagcaa cgactgtttg 6301 cccgccagtt gttgtgccac gcggttggga atgtaattca gctccgccat cgccgcttcc 6361 acttcttccc gcgttttcgc agaaacgtgg ctggcctggt tcaccacgcg ggaaacggtc 6421 tgataagaga caccggcata ctctgcgaca tcgtataacg ttactggttt cacctagtac 6481 tttcctgtgt gactctagta gcaaccatta tcaccgccag aggtaaaata gtcaacacgc 6541 acggtgttaa gagcgctgga ttactacccg ggacctatac tacaatacgc aaaccgcctc 6601 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag 6661 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt 6721 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca 6781 catactagag tcacacagga aagtactaga tgagcacaaa aaagaaacca ttaacacaag 6841 agcagcttga ggacgcacgt cgccttaaag caatttatga aaaaaagaaa aatgaacttg 6901 gcttatccca ggaatctgtc gcagacaaga tggggatggg gcagtcaggc gttggtgctt 6961 tatttaatgg catcaatgca ttaaatgctt ataacgccgc attgcttaca aaaattctca 7021 aagttagcgt tgaagaattt agcccttcaa tcgccagaga aatctacgag atgtatgaag 7081 cggttagtat gcagccgtca cttagaagtg agtatgagta ccctgttttt tctcatgttc 7141 aggcagggat gttctcacct gagcttagaa cctttaccaa aggtgatgcg gagagatggg 7201 taagcacaac caaaaaagcc agtgattctg cattctggct tgaggttgaa ggtaattcca 7261 tgaccgcacc aacaggctcc aagccaagct ttcctgacgg aatgttaatt ctcgttgacc 7321 ctgagcaggc tgttgagcca ggtgatttct gcatagccag acttgggggt gatgagttta 7381 ccttcaagaa actgatcagg gatagcggtc aggtgttttt acaaccacta aacccacagt 7441 acccaatgat cccatgcaat gagagttgtt ccgttgtggg gaaagttatc gctagtcagt 7501 ggcctgaaga gacgtttggc tgatagctag caggtaggat gcctttgatt caacgaatct 7561 gtagaagttc aatcttttgc aaataagtta agtttttaag gataaaaaaa tgaaaagaaa 7621 agtattggca atggatttgt cacagctaac accacgtcgt ccctatctgc tgcgtgcatt 7681 ctatgagtgg ttgctggata accagctcac gccgcacctg gtggtggatg tgacgctccc 7741 aggcgtgcag gttcctatgg aatatgcgcg tgacgggcaa atcgtactca acattgcgcc 7801 gcgtgctgtc ggcaatctgg aactggcgaa tgatgaggtg cgctttaacg cgcgctttgg 7861 tggcattccg cgtcaggttt ctgtgccgct ggctgccgtg ctggctatct acgcccgtga 7921 aaatggtgca ggcacgatgt ttgagcctga agctgcctac gatgaagatg ccagcatcat 7981 gaatgatgaa gaggcatcgg cagacaacga aaccgttatg tcggttatcg atggcgacaa 8041 gccagatcac gatgatgaaa ctcatcctga cgatgaacct ccgcagccac cacgcggtgg 8101 tcgaccggca ttacgcgttg tgaagtaata ccgcgggtag gatgcctttg attcaacgaa 8161 tctgtagaag ttcaatcttt tgcaaataag ttaagttttt aaggataaaa aaatgaaaag 8221 aaaagtattg gcaatggctt cctccgaaga cgttatcaaa gagttcatgc gtttcaaagt 8281 tcgtatggaa ggttccgtta acggtcacga gttcgaaatc gaaggtgaag gtgaaggtcg 8341 tccgtacgaa ggtacccaga ccgctaaact gaaagttacc aaaggtggtc cgctgccgtt 8401 cgcttgggac atcctgtccc cgcagttcca gtacggttcc aaagcttacg ttaaacaccc 8461 ggctgacatc ccggactacc tgaaactgtc cttcccggaa ggtttcaaat gggaacgtgt 8521 tatgaacttc gaagacggtg gtgttgttac cgttacccag gactcctccc tgcaagacgg 8581 tgagttcatc tacaaagtta aactgcgtgg taccaacttc ccgtccgacg gtccggttat 8641 gcagaaaaaa accatgggtt gggaagcttc caccgaacgt atgtacccgg aagacggtgc 8701 tctgaaaggt gaaatcaaaa tgcgtctgaa actgaaagac ggtggtcact acgacgctga 8761 agttaaaacc acctacatgg ctaaaaaacc ggttcagctg ccgggtgctt acaaaaccga 8821 catcaaactg gacatcacct cccacaacga agactacacc atcgttgaac agtacgaacg 8881 tgctgaaggt cgtcactcca ccggtgctta ataatgatac taggcctagc caggcatcaa 8941 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg 9001 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata 9061 ctagagtact agag //