##gff-version 3
##sequence-region BBa_K510045 1 9074
BBa_K510045	.	plasmid	1	4388	.	+	0	ID=BBa_K510000;Name=BBa_K510000
BBa_K510045	.	engineered_region	4367	4387	.	+	0	ID=annotation2137423;Name=Prefix;Parent=BBa_K510000
BBa_K510045	.	sequence_feature	4196	4243	.	+	0	ID=annotation2137422;Name=Flipase recombination sequence (FRT);Parent=BBa_K510000
BBa_K510045	.	CDS	966	1826	.	+	0	ID=annotation2137416;Name=Ampicillin resistance (bla);Parent=BBa_K510000
BBa_K510045	.	sequence_feature	182	347	.	+	0	ID=annotation2137414;Name=Tn7-L end;Parent=BBa_K510000
BBa_K510045	.	stem_loop	4326	4359	.	+	0	ID=annotation2137449;Name=Terminator;Parent=BBa_K510000
BBa_K510045	.	sequence_feature	348	360	.	+	0	ID=annotation2137415;Name=SfiI restriction site;Parent=BBa_K510000
BBa_K510045	.	sequence_feature	4190	4195	.	+	0	ID=annotation2137440;Name=SphI restriction site;Parent=BBa_K510000
BBa_K510045	.	sequence_feature	3002	3014	.	+	0	ID=annotation2137418;Name=SfiI restriction site;Parent=BBa_K510000
BBa_K510045	.	CDS	3393	3926	.	+	0	ID=annotation2137421;Name=Gentamycin resistance;Parent=BBa_K510000
BBa_K510045	.	primer_binding_site	157	176	.	+	0	ID=annotation2137413;Name=VR (BBa_G00101) primer binding site;Parent=BBa_K510000
BBa_K510045	.	sequence_feature	3381	3386	.	+	0	ID=annotation2137425;Name=SphI restriction site;Parent=BBa_K510000
BBa_K510045	.	sequence_feature	3387	3392	.	+	0	ID=annotation2137436;Name=NcoI restrictino site;Parent=BBa_K510000
BBa_K510045	.	sequence_feature	4184	4189	.	+	0	ID=annotation2137439;Name=NcoI restriction site;Parent=BBa_K510000
BBa_K510045	.	sequence_feature	3014	3213	.	+	0	ID=annotation2137419;Name=Tn7-R end;Parent=BBa_K510000
BBa_K510045	.	engineered_region	2	21	.	+	0	ID=annotation2137411;Name=Suffix;Parent=BBa_K510000
BBa_K510045	.	sequence_feature	3329	3376	.	+	0	ID=annotation2137420;Name=Flipase recombination sequence (FRT);Parent=BBa_K510000
BBa_K510045	.	engineered_region	3224	3318	.	+	0	ID=annotation2137424;Name=double terminator (BBa_B0014);Parent=BBa_K510000
BBa_K510045	.	sequence_feature	1965	2644	.	+	0	ID=annotation2137417;Name=ColEI (pUC18) Replication origin;Parent=BBa_K510000
BBa_K510045	.	stem_loop	22	93	.	+	0	ID=annotation2137412;Name=E. coli his operon terminator;Parent=BBa_K510000
BBa_K510045	.	primer_binding_site	4249	4268	.	+	0	ID=annotation2137442;Name=VF2 (G00100) primer binding site;Parent=BBa_K510000
BBa_K510045	.	engineered_region	4397	9074	.	+	0	ID=BBa_K510019;Name=BBa_K510019
BBa_K510045	.	CDS	7535	8128	.	+	0	ID=annotation2136074;Name=RBS+OmpN::SspB;Parent=BBa_K510019
BBa_K510045	.	sequence_feature	4543	4550	.	+	0	ID=annotation2136060;Name=Restriction Site: AscI;Parent=BBa_K510019
BBa_K510045	.	sequence_feature	7535	7630	.	+	0	ID=annotation2136075;Name=RBS+OmpN;Parent=BBa_K510019
BBa_K510045	.	sequence_feature	8138	8233	.	+	0	ID=annotation2136077;Name=RBS+OmpN;Parent=BBa_K510019
BBa_K510045	.	tag	5349	5393	.	+	0	ID=annotation2136066;Name=DAS4;Parent=BBa_K510019
BBa_K510045	.	sequence_feature	8922	8927	.	+	0	ID=annotation2136079;Name=Restriction Site: StuI;Parent=BBa_K510019
BBa_K510045	.	tag	4557	4601	.	+	0	ID=annotation2136062;Name=DAS4;Parent=BBa_K510019
BBa_K510045	.	stop_codon	8930	9058	.	+	0	ID=annotation2136080;Name=Double terminator;Parent=BBa_K510019
BBa_K510045	.	CDS	8138	8914	.	+	0	ID=annotation2136078;Name=RBS+OmpN::RFP;Parent=BBa_K510019
BBa_K510045	.	ribosome_entry_site	6791	6803	.	+	0	ID=annotation2136071;Name=RBS;Parent=BBa_K510019
BBa_K510045	.	CDS	4551	5315	.	+	0	ID=annotation2136061;Name=GFP::Das4;Parent=BBa_K510019
BBa_K510045	.	sequence_feature	5336	5341	.	+	0	ID=annotation2136064;Name=Restriction Site: BamHI;Parent=BBa_K510019
BBa_K510045	.	promoter	6583	6782	.	+	0	ID=annotation2136070;Name=PLac;Parent=BBa_K510019
BBa_K510045	.	sequence_feature	7526	7531	.	+	0	ID=annotation2136073;Name=Restriction Site: NheI;Parent=BBa_K510019
BBa_K510045	.	sequence_feature	8131	8136	.	+	0	ID=annotation2136076;Name=Restriction Site: SacII;Parent=BBa_K510019
BBa_K510045	.	CDS	6810	7523	.	+	0	ID=annotation2136072;Name=cI (Ts);Parent=BBa_K510019
BBa_K510045	.	ribosome_entry_site	5322	5334	.	+	0	ID=annotation2136063;Name=RBS;Parent=BBa_K510019
BBa_K510045	.	sequence_feature	6567	6572	.	+	0	ID=annotation2136069;Name=Restriction Site: Sma I;Parent=BBa_K510019
BBa_K510045	.	stop_codon	4406	4534	.	+	0	ID=annotation2136058;Name=Double terminator;Parent=BBa_K510019
BBa_K510045	.	promoter	6501	6549	.	+	0	ID=annotation2136068;Name=PcI;Parent=BBa_K510019
BBa_K510045	.	sequence_feature	4535	4540	.	+	0	ID=annotation2136059;Name=Restriction Site: AflII;Parent=BBa_K510019
BBa_K510045	.	CDS	5343	6473	.	+	0	ID=annotation2136065;Name=LacI::Das4;Parent=BBa_K510019
BBa_K510045	.	ribosome_entry_site	6480	6492	.	+	0	ID=annotation2136067;Name=RBS;Parent=BBa_K510019
>BBa_K510045
tactagtagcggccgctgcagtccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaa
gattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagc
tcactcaaaggcggtaataggccaaccagataagtgaaatctagttccaaactattttgtcatttttaattttcgtatt
agcttacgacgctacacccagttcccatctattttgtcactcttccctaaataatccttaaaaactccatttccacccc
tcccagttcccaactattttgtccgcccacaggccgcctaggccgtagcttggcactggccgtcgttttacaacgtcgt
gactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaag
aggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgcctgatgcggtattttctccttac
gcatctgtgcggtatttcacaccgcatatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagccc
cgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccg
tctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgagacgaaagggcctcgtgatacgcct
atttttataggttaatgtcatgataataatggtttcttagacgtcaggtggcacttttcggggaaatgtgcgcggaacc
cctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataat
attgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgt
ttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactg
gatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgc
tatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgactt
ggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataacc
atgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaaca
tgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccac
gatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaatta
atagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgata
aatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagt
tatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaag
cattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatct
aggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgt
agaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgcta
ccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagatac
caaatactgttcttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctct
gctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccg
gataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactga
gatacctacagcgtgagctttgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcag
ggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccac
ctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttt
tacggttcctggccttttgctggccttttgctcacatgttctttcctgcgttatcccctgattctgtggataaccgtat
taccgcctttgagtgagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaa
gagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgact
ggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgct
tccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacaggaaacagctatgaccatgattacgaattg
gccgcctaggcctgtgggcggacaataaagtcttaaactgaacaaaatagatctaaactatgacaataaagtcttaaac
tagacagaatagttgtaaactgaaatcagtccagttatgctgtgaaaaagcatactggacttttgttatggctaaagca
aactcttcattttctgaagtgcaaattgcccgtcgtattaaagaggggcgtggggttcgcttgtcacactggctcacct
tcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttattattt
gctagatcttgaagtacctattccgaagttcctattctctaaaaagtataggaacttcagaggcatgcccatggttagg
tggcggtacttgggtcgatatcaaagtgcatcacttcttcccgtatgcccaactttgtatagagagccactgcgggatc
gtcaccgtaatctgcttgcacgtagatcacataagcaccaagcgcgttggcctcatgcttgaggagattgatgagcgcg
gtggcaatgccctgcctccggtgctcgccggagactgcgagatcatagatatagatctcactacgcggctgctcaaact
tgggcagaacgtaagccgcgagagcgccaacaaccgcttcttggtcgaaggcagcaagcgcgatgaatgtcttactacg
gagcaagttcccgaggtaatcggagtccggctgatgttgggagtaggtggctacgtctccgaactcacgaccgaaaaga
tcaagagcagcccgcatggatttgacttggtcagggccgagcctacatgtgcgaatgatgcccatacttgagccaccta
actttgttttagggcgactgccctgctgcgtaacatcgttgctgctgcgtaacatcgttgctgctccataacatcaaac
atcgacccacggcgtaacgcgcttgctgcttggatgcccgaggcatagactgtacaaaaaaacagtcataacaagccat
gaaaaccgccactgcgccgttaccaccgctgcgttcggtcaaggttctggaccagttgcgtgagcgcatacgctacttg
cattacagtttacgaaccgaacaggcttatgtcaattcgatccattgctgttgacaaagggaatcaggggatcttccat
gggcatgcgaagtacctattccgaagttcctattctctaaaaagtataggaacttcagagctgccacctgacgtctaag
aaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagc
tttcgctaaggatgatttctggaattcgcggccgcttctagagtactagaggagatcatttataaacgcagaaaggccc
acccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttc
gactgagcctttcgttttatttgatgcctggcttaagtaggcgcgccctattaagaagcatcagcataattttcagagt
agttctcgtcgtttgctgctttgtatagttcatccatgccatgtgtaatcccagcagctgttacaaactcaagaaggac
catgtggtctctcttttcgttgggatctttcgaaagggcagattgtgtggacaggtaatggttgtctggtaaaaggaca
gggccatcgccaattggagtattttgttgataatggtctgctagttgaacgcttccatcttcaatgttgtgtctaattt
tgaagttaactttgattccattcttttgtttgtctgccatgatgtatacattgtgtgagttatagttgtattccaattt
gtgtccaagaatgtttccatcttctttaaaatcaataccttttaactcgattctattaacaagggtatcaccttcaaac
ttgacttcagcacgtgtcttgtagttcccgtcatctttgaaaaatatagttctttcctgtacataaccttcaggcatgg
cactcttgaaaaagtcatgctgtttcatatgatctgggtatctcgcaaagcattgaacaccataaccgaaagtagtgac
aagtgttggccatggaacaggtagttttccagtagtgcaaataaatttaagggtaagttttccgtatgttgcatcacct
tcaccctctccactgacagaaaatttgtgcccattaacatcaccatctaattcaacaagaattgggacaactccagtga
aaagttcttctcctttactcatctagtactttcctgtgtgacggatccactatcaagaagcatcagcataattttcaga
gtagttctcgtcgtttgctgcctgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaac
gcgcggggagaggcggtttgcgtattgggcgccagggtggtttttcttttcaccagtgagacgggcaacagctgattgc
ccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgccccagcaggcgaaaatcctgtttgat
ggtggttaacggcgggatataacatgagctgtcttcggtatcgtcgtatcccactaccgagatatccgcaccaacgcgc
agcccggactcggtaatggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacgatgc
cctcattcagcatttgcatggtttgttgaaaaccggacatggcactccagtcgccttcccgttccgctatcggctgaat
ttgattgcgagtgagatatttatgccagccagccagacgcagacgcgccgagacagaacttaatgggcccgctaacagc
gcgatttgctggtgacccaatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactgt
tgatgggtgtctggtcagagacatcaagaaataacgccggaacattagtgcaggcagcttccacagcaatggcatcctg
gtcatccagcggatagttaatgatcagcccactgacgcgttgcgcgagaagattgtgcaccgccgctttacaggcttcg
acgccgcttcgttctaccatcgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaattt
gcgacggcgcgtgcagggccagactggaggtggcaacgccaatcagcaacgactgtttgcccgccagttgttgtgccac
gcggttgggaatgtaattcagctccgccatcgccgcttccacttcttcccgcgttttcgcagaaacgtggctggcctgg
ttcaccacgcgggaaacggtctgataagagacaccggcatactctgcgacatcgtataacgttactggtttcacctagt
actttcctgtgtgactctagtagcaaccattatcaccgccagaggtaaaatagtcaacacgcacggtgttaagagcgct
ggattactacccgggacctatactacaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggc
acgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcacccca
ggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagtcac
acaggaaagtactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaa
tttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgt
tggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttacaaaaattctcaaagttagcgtt
gaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtg
agtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgc
ggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgca
ccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatt
tctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaacc
actaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcct
gaagagacgtttggctgatagctagcaggtaggatgcctttgattcaacgaatctgtagaagttcaatcttttgcaaat
aagttaagtttttaaggataaaaaaatgaaaagaaaagtattggcaatggatttgtcacagctaacaccacgtcgtccc
tatctgctgcgtgcattctatgagtggttgctggataaccagctcacgccgcacctggtggtggatgtgacgctcccag
gcgtgcaggttcctatggaatatgcgcgtgacgggcaaatcgtactcaacattgcgccgcgtgctgtcggcaatctgga
actggcgaatgatgaggtgcgctttaacgcgcgctttggtggcattccgcgtcaggtttctgtgccgctggctgccgtg
ctggctatctacgcccgtgaaaatggtgcaggcacgatgtttgagcctgaagctgcctacgatgaagatgccagcatca
tgaatgatgaagaggcatcggcagacaacgaaaccgttatgtcggttatcgatggcgacaagccagatcacgatgatga
aactcatcctgacgatgaacctccgcagccaccacgcggtggtcgaccggcattacgcgttgtgaagtaataccgcggg
taggatgcctttgattcaacgaatctgtagaagttcaatcttttgcaaataagttaagtttttaaggataaaaaaatga
aaagaaaagtattggcaatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttc
cgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaa
gttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgtta
aacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcga
agacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggt
accaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccgg
aagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaac
cacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaac
gaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataatgatactaggcct
agccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgct
ctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtactagag