BBa_K510045
type: ComponentDefinition
persistent identity: BBa K510045
display id: BBa_K510045
version: 1
was generated by: iGEM to SBOL conversion
description: pUC18Sfi-miniTn7BB-Gm-improved_flipflop_(module I)
created: 2011-10-21T11:00:00Z
modified: 2015-05-08T01:12:30Z
mutable provenance: The miniTn7BB-Gm minitransposon was synthesized commercially and pUC18SfiI is a commercial vector. The improved flip-flop (module I) was synthesized commercially.
owned by: james
owned by: myers
mutable description: This plasmid allows the integration of the improved flip-flop (module I) (part: BBa_K510019) into bacterial chromosomes using the pUC18Sfi-miniTn7BB-Gm (BBa_K51000) characteristics. Single copy may improve the function of regulatory circuits, as bistable systems. For more information about the function of the miniTn7 BioBrick toolkit, visit the iGEM team UPO-Sevilla 2011 wiki.
discontinued: false
dominant: false
experience: experience/None
group u list: _673_
m user id: 0
owner id: 6163
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: The BBa_K510019 BioBrick was inserted within the BCS of pUC18Sfi-miniTn7BB-Gm (BBa K510000) by EcoRI and PstI clonning. In order to construct the pUC18Sfi-miniTn7BB-Gm delivery plasmid, the miniTn7BB-Gm transposon was cleaved from the commercial plasmid pMA (Mr. Gene) with SfiI and ligated to SfiI-digested pUC18Sfi. This strategy removes all multi-cloning restriction sites from pUC18Sfi, except for the flanking SfiI, thus guaranteeing that the unique sites in the transposon are not duplicated. The remove of the multi-cloning restriction sites was verified by analytic digestions.
star: false
creator: David Caballero, Felix Reyes
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K510000
component: BBa_K510019
sequence annotation: BBa_K510000
sequence annotation: BBa_K510019
sequence: https://synbiohub.org/public/igem/BBa_K510045_sequence/1

BBa_K510000
type: Component
persistent identity: component2160037
display id: component2160037
version: 1
top level: BBa_K510045
definition: BBa_K510000
access: public

BBa_K510019
type: Component
persistent identity: component2160061
display id: component2160061
version: 1
top level: BBa_K510045
definition: BBa_K510019
access: public

BBa_K510000
type: SequenceAnnotation
persistent identity: annotation2160037
display id: annotation2160037
version: 1
top level: BBa_K510045
location: https://synbiohub.org/public/igem/BBa_K510045/annotation2160037/range2160037/1
component: BBa_K510000

https://synbiohub.org/public/igem/BBa_K510045/annotation2160037/range2160037/1
type: Range
persistent identity: range2160037
display id: range2160037
version: 1
top level: BBa_K510045
direction: direction/reverse
start: 1
end: 4388
orientation: inline

BBa_K510019
type: SequenceAnnotation
persistent identity: annotation2160061
display id: annotation2160061
version: 1
top level: BBa_K510045
location: https://synbiohub.org/public/igem/BBa_K510045/annotation2160061/range2160061/1
component: BBa_K510019

https://synbiohub.org/public/igem/BBa_K510045/annotation2160061/range2160061/1
type: Range
persistent identity: range2160061
display id: range2160061
version: 1
top level: BBa_K510045
direction: direction/reverse
start: 4397
end: 9074
orientation: inline

BBa_K510000
type: ComponentDefinition
persistent identity: BBa K510000
display id: BBa_K510000
version: 1
was generated by: iGEM to SBOL conversion
description: pUC18Sfi-miniTn7BB-Gm
created: 2011-09-15T11:00:00Z
modified: 2015-05-08T01:12:30Z
mutable provenance: The pUC18-SfiI cloning vector was kindly provided by the Microbiology laboratory of Centro Andaluz de Biolog??a del Desarrollo (CABD) in Sevilla. The miniTn7BB-Gm synthetic transposon was designed by the iGEM 2011 team UPO-Sevilla based on the set of miniTn7 derivatives developed by Choi et al. (2005) and commercially synthesized by Mr. Gene (Regensburg, Germany). K.-H. Choi, J. B. Gaynor, K. G. White, C. Lopez, C. M. Bosio, R. R. Karkhoff-Schweizer & H. P. Schweizer (2005). A Tn7-based broad-range bacterial cloning and expression system. Nature Methods, vol.2 NO.6, June 2005, 443.
owned by: james
owned by: myers
mutable description: The miniTn7BB-Gm synthetic minitransposon was digested at the flanking SfiI sites and cloned into SfiI-digested pUC18-Sfi. This results in the elimination of the complete pUC18-SfiI multi-cloning site, except for the duplicated SfiI sites that remain on both sides of the transposon, thus facilitating its transfer to other vectors. This plasmid can be used to keep the mini-Tn7-Gm in E. coli and other enterics, and may be used for transposition into the genomes of non-enteric bacteria, in which it is non-replicative. The structure of the mini-Tn7BB-Gm transposon is shown in the figure below. Tn7R and Tn7L are the right and left ends of Tn7 transposon, respectively, required for recognition of the transposase machinery and transposition. FRT is the Flp recombinase target site for excision of the resistance once the transposon is inserted in the genome of a living organism. The gentamycin resistance cassette (Gm) is flanked by restriction sites in order to facilitate the generation of variants with different antibiotic resistance markers. The BioBrick cloning site (BCS) includes the prefix and suffix restrictions sites used in assembly standard 10, and it is the place where BioBricks are inserted for genome integration. The complete construction is flanked by SfiI restriction sites to facilitate cloning.
discontinued: false
dominant: false
experience: experience/Works
group u list: _673_
m user id: 0
owner id: 6157
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: In order to make this vector compatible with assembly standard 10, several restriction sites were removed from the synthetic sequence.
star: false
creator: David Caballero, Fernando Govantes
type: DnaRegion
role: partType/Plasmid
role: SO:0000155
sequence annotation: Suffix
sequence annotation: E. coli his operon terminator
sequence annotation: VR (BBa_G00101) primer binding site
sequence annotation: Tn7-L end
sequence annotation: SfiI restriction site
sequence annotation: Ampicillin resistance (bla)
sequence annotation: ColEI (pUC18) Replication origin
sequence annotation: Tn7-R end
sequence annotation: double terminator (BBa_B0014)
sequence annotation: Flipase recombination sequence (FRT)
sequence annotation: SphI restriction site
sequence annotation: NcoI restrictino site
sequence annotation: Gentamycin resistance
sequence annotation: NcoI restriction site
sequence annotation: VF2 (G00100) primer binding site
sequence annotation: Terminator
sequence annotation: Prefix
sequence: https://synbiohub.org/public/igem/BBa_K510000_sequence/1

Suffix
type: SequenceAnnotation
persistent identity: annotation2137411
display id: annotation2137411
version: 1
top level: BBa_K510000
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137411/range2137411/1
role: SO:0000804
role: feature/BioBrick

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type: Range
persistent identity: range2137411
display id: range2137411
version: 1
top level: BBa_K510000
direction: direction/forward
start: 2
end: 21
orientation: inline

E. coli his operon terminator
type: SequenceAnnotation
persistent identity: annotation2137412
display id: annotation2137412
version: 1
top level: BBa_K510000
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137412/range2137412/1
role: SO:0000313
role: feature/stem loop

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type: Range
persistent identity: range2137412
display id: range2137412
version: 1
top level: BBa_K510000
direction: direction/forward
start: 22
end: 93
orientation: inline

VR (BBa_G00101) primer binding site
type: SequenceAnnotation
persistent identity: annotation2137413
display id: annotation2137413
version: 1
top level: BBa_K510000
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137413/range2137413/1
role: feature/primer binding
role: SO:0005850

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type: Range
persistent identity: range2137413
display id: range2137413
version: 1
top level: BBa_K510000
direction: direction/forward
start: 157
end: 176
orientation: inline

Tn7-L end
type: SequenceAnnotation
persistent identity: annotation2137414
display id: annotation2137414
version: 1
top level: BBa_K510000
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137414/range2137414/1
role: feature/misc
role: SO:0000110

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type: Range
persistent identity: range2137414
display id: range2137414
version: 1
top level: BBa_K510000
direction: direction/forward
start: 182
end: 347
orientation: inline

SfiI restriction site
type: SequenceAnnotation
persistent identity: annotation2137415
display id: annotation2137415
version: 1
top level: BBa_K510000
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137415/range2137415/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K510000/annotation2137415/range2137415/1
type: Range
persistent identity: range2137415
display id: range2137415
version: 1
top level: BBa_K510000
direction: direction/forward
start: 348
end: 360
orientation: inline

Ampicillin resistance (bla)
type: SequenceAnnotation
persistent identity: annotation2137416
display id: annotation2137416
version: 1
top level: BBa_K510000
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137416/range2137416/1
role: feature/cds
role: SO:0000316

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type: Range
persistent identity: range2137416
display id: range2137416
version: 1
top level: BBa_K510000
direction: direction/forward
start: 966
end: 1826
orientation: inline

ColEI (pUC18) Replication origin
type: SequenceAnnotation
persistent identity: annotation2137417
display id: annotation2137417
version: 1
top level: BBa_K510000
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137417/range2137417/1
role: feature/dna
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K510000/annotation2137417/range2137417/1
type: Range
persistent identity: range2137417
display id: range2137417
version: 1
top level: BBa_K510000
direction: direction/forward
start: 1965
end: 2644
orientation: inline

SfiI restriction site
type: SequenceAnnotation
persistent identity: annotation2137418
display id: annotation2137418
version: 1
top level: BBa_K510000
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137418/range2137418/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K510000/annotation2137418/range2137418/1
type: Range
persistent identity: range2137418
display id: range2137418
version: 1
top level: BBa_K510000
direction: direction/forward
start: 3002
end: 3014
orientation: inline

Tn7-R end
type: SequenceAnnotation
persistent identity: annotation2137419
display id: annotation2137419
version: 1
top level: BBa_K510000
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137419/range2137419/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K510000/annotation2137419/range2137419/1
type: Range
persistent identity: range2137419
display id: range2137419
version: 1
top level: BBa_K510000
direction: direction/forward
start: 3014
end: 3213
orientation: inline

double terminator (BBa_B0014)
type: SequenceAnnotation
persistent identity: annotation2137424
display id: annotation2137424
version: 1
top level: BBa_K510000
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137424/range2137424/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K510000/annotation2137424/range2137424/1
type: Range
persistent identity: range2137424
display id: range2137424
version: 1
top level: BBa_K510000
direction: direction/forward
start: 3224
end: 3318
orientation: inline

Flipase recombination sequence (FRT)
type: SequenceAnnotation
persistent identity: annotation2137420
display id: annotation2137420
version: 1
top level: BBa_K510000
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137420/range2137420/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K510000/annotation2137420/range2137420/1
type: Range
persistent identity: range2137420
display id: range2137420
version: 1
top level: BBa_K510000
direction: direction/forward
start: 3329
end: 3376
orientation: inline

SphI restriction site
type: SequenceAnnotation
persistent identity: annotation2137425
display id: annotation2137425
version: 1
top level: BBa_K510000
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137425/range2137425/1
role: SO:0000110
role: feature/dna

https://synbiohub.org/public/igem/BBa_K510000/annotation2137425/range2137425/1
type: Range
persistent identity: range2137425
display id: range2137425
version: 1
top level: BBa_K510000
direction: direction/forward
start: 3381
end: 3386
orientation: inline

NcoI restrictino site
type: SequenceAnnotation
persistent identity: annotation2137436
display id: annotation2137436
version: 1
top level: BBa_K510000
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137436/range2137436/1
role: feature/dna
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K510000/annotation2137436/range2137436/1
type: Range
persistent identity: range2137436
display id: range2137436
version: 1
top level: BBa_K510000
direction: direction/forward
start: 3387
end: 3392
orientation: inline

Gentamycin resistance
type: SequenceAnnotation
persistent identity: annotation2137421
display id: annotation2137421
version: 1
top level: BBa_K510000
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137421/range2137421/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K510000/annotation2137421/range2137421/1
type: Range
persistent identity: range2137421
display id: range2137421
version: 1
top level: BBa_K510000
direction: direction/reverse
start: 3393
end: 3926
orientation: inline

NcoI restriction site
type: SequenceAnnotation
persistent identity: annotation2137439
display id: annotation2137439
version: 1
top level: BBa_K510000
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137439/range2137439/1
role: SO:0000110
role: feature/dna

https://synbiohub.org/public/igem/BBa_K510000/annotation2137439/range2137439/1
type: Range
persistent identity: range2137439
display id: range2137439
version: 1
top level: BBa_K510000
direction: direction/forward
start: 4184
end: 4189
orientation: inline

SphI restriction site
type: SequenceAnnotation
persistent identity: annotation2137440
display id: annotation2137440
version: 1
top level: BBa_K510000
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137440/range2137440/1
role: SO:0000110
role: feature/dna

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type: Range
persistent identity: range2137440
display id: range2137440
version: 1
top level: BBa_K510000
direction: direction/forward
start: 4190
end: 4195
orientation: inline

Flipase recombination sequence (FRT)
type: SequenceAnnotation
persistent identity: annotation2137422
display id: annotation2137422
version: 1
top level: BBa_K510000
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137422/range2137422/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K510000/annotation2137422/range2137422/1
type: Range
persistent identity: range2137422
display id: range2137422
version: 1
top level: BBa_K510000
direction: direction/forward
start: 4196
end: 4243
orientation: inline

VF2 (G00100) primer binding site
type: SequenceAnnotation
persistent identity: annotation2137442
display id: annotation2137442
version: 1
top level: BBa_K510000
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137442/range2137442/1
role: feature/primer binding
role: SO:0005850

https://synbiohub.org/public/igem/BBa_K510000/annotation2137442/range2137442/1
type: Range
persistent identity: range2137442
display id: range2137442
version: 1
top level: BBa_K510000
direction: direction/forward
start: 4249
end: 4268
orientation: inline

Terminator
type: SequenceAnnotation
persistent identity: annotation2137449
display id: annotation2137449
version: 1
top level: BBa_K510000
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137449/range2137449/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_K510000/annotation2137449/range2137449/1
type: Range
persistent identity: range2137449
display id: range2137449
version: 1
top level: BBa_K510000
direction: direction/forward
start: 4326
end: 4359
orientation: inline

Prefix
type: SequenceAnnotation
persistent identity: annotation2137423
display id: annotation2137423
version: 1
top level: BBa_K510000
location: https://synbiohub.org/public/igem/BBa_K510000/annotation2137423/range2137423/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K510000/annotation2137423/range2137423/1
type: Range
persistent identity: range2137423
display id: range2137423
version: 1
top level: BBa_K510000
direction: direction/forward
start: 4367
end: 4387
orientation: inline

BBa_K510019
type: ComponentDefinition
persistent identity: BBa K510019
display id: BBa_K510019
version: 1
was generated by: iGEM to SBOL conversion
description: Improved Flip Flop (Module I)
created: 2011-09-18T11:00:00Z
modified: 2015-05-08T01:12:30Z
mutable provenance: It's a part which comes from different genes forming the bassic flip-flop with anothers improvements.
owned by: james
owned by: myers
mutable description: Here we show the final structure of our improved bistable. As well as the two operons with their protein fusions and the promoter with the asRNA, we show the different restriction sites we have introduced and the enzymes that target each site. Finally, the total length of our construction is indicated in the figure.
discontinued: false
dominant: false
experience: experience/Works
group u list: _673_
m user id: 0
owner id: 6159
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
star: false
creator: Victor Alvarez, Felix Reyes and Yolanda Gonzalez
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: Double terminator
sequence annotation: Restriction Site: AflII
sequence annotation: Restriction Site: AscI
sequence annotation: GFP::Das4
sequence annotation: DAS4
sequence annotation: RBS
sequence annotation: Restriction Site: BamHI
sequence annotation: LacI::Das4
sequence annotation: PcI
sequence annotation: Restriction Site: Sma I
sequence annotation: PLac
sequence annotation: cI (Ts)
sequence annotation: Restriction Site: NheI
sequence annotation: RBS+OmpN::SspB
sequence annotation: RBS+OmpN
sequence annotation: Restriction Site: SacII
sequence annotation: RBS+OmpN::RFP
sequence annotation: Restriction Site: StuI
sequence: https://synbiohub.org/public/igem/BBa_K510019_sequence/1

Double terminator
type: SequenceAnnotation
persistent identity: annotation2136058
display id: annotation2136058
version: 1
top level: BBa_K510019
location: https://synbiohub.org/public/igem/BBa_K510019/annotation2136058/range2136058/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K510019/annotation2136058/range2136058/1
type: Range
persistent identity: range2136058
display id: range2136058
version: 1
top level: BBa_K510019
direction: direction/reverse
start: 10
end: 138
orientation: inline

Restriction Site: AflII
type: SequenceAnnotation
persistent identity: annotation2136059
display id: annotation2136059
version: 1
top level: BBa_K510019
location: https://synbiohub.org/public/igem/BBa_K510019/annotation2136059/range2136059/1
role: feature/dna
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K510019/annotation2136059/range2136059/1
type: Range
persistent identity: range2136059
display id: range2136059
version: 1
top level: BBa_K510019
direction: direction/forward
start: 139
end: 144
orientation: inline

Restriction Site: AscI
type: SequenceAnnotation
persistent identity: annotation2136060
display id: annotation2136060
version: 1
top level: BBa_K510019
location: https://synbiohub.org/public/igem/BBa_K510019/annotation2136060/range2136060/1
role: feature/dna
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K510019/annotation2136060/range2136060/1
type: Range
persistent identity: range2136060
display id: range2136060
version: 1
top level: BBa_K510019
direction: direction/forward
start: 147
end: 154
orientation: inline

GFP::Das4
type: SequenceAnnotation
persistent identity: annotation2136061
display id: annotation2136061
version: 1
top level: BBa_K510019
location: https://synbiohub.org/public/igem/BBa_K510019/annotation2136061/range2136061/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K510019/annotation2136061/range2136061/1
type: Range
persistent identity: range2136061
display id: range2136061
version: 1
top level: BBa_K510019
direction: direction/reverse
start: 155
end: 919
orientation: inline

DAS4
type: SequenceAnnotation
persistent identity: annotation2136062
display id: annotation2136062
version: 1
top level: BBa_K510019
location: https://synbiohub.org/public/igem/BBa_K510019/annotation2136062/range2136062/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_K510019/annotation2136062/range2136062/1
type: Range
persistent identity: range2136062
display id: range2136062
version: 1
top level: BBa_K510019
direction: direction/reverse
start: 161
end: 205
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2136063
display id: annotation2136063
version: 1
top level: BBa_K510019
location: https://synbiohub.org/public/igem/BBa_K510019/annotation2136063/range2136063/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K510019/annotation2136063/range2136063/1
type: Range
persistent identity: range2136063
display id: range2136063
version: 1
top level: BBa_K510019
direction: direction/reverse
start: 926
end: 938
orientation: inline

Restriction Site: BamHI
type: SequenceAnnotation
persistent identity: annotation2136064
display id: annotation2136064
version: 1
top level: BBa_K510019
location: https://synbiohub.org/public/igem/BBa_K510019/annotation2136064/range2136064/1
role: SO:0000110
role: feature/dna

https://synbiohub.org/public/igem/BBa_K510019/annotation2136064/range2136064/1
type: Range
persistent identity: range2136064
display id: range2136064
version: 1
top level: BBa_K510019
direction: direction/forward
start: 940
end: 945
orientation: inline

LacI::Das4
type: SequenceAnnotation
persistent identity: annotation2136065
display id: annotation2136065
version: 1
top level: BBa_K510019
location: https://synbiohub.org/public/igem/BBa_K510019/annotation2136065/range2136065/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K510019/annotation2136065/range2136065/1
type: Range
persistent identity: range2136065
display id: range2136065
version: 1
top level: BBa_K510019
direction: direction/reverse
start: 947
end: 2077
orientation: inline

DAS4
type: SequenceAnnotation
persistent identity: annotation2136066
display id: annotation2136066
version: 1
top level: BBa_K510019
location: https://synbiohub.org/public/igem/BBa_K510019/annotation2136066/range2136066/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_K510019/annotation2136066/range2136066/1
type: Range
persistent identity: range2136066
display id: range2136066
version: 1
top level: BBa_K510019
direction: direction/reverse
start: 953
end: 997
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2136067
display id: annotation2136067
version: 1
top level: BBa_K510019
location: https://synbiohub.org/public/igem/BBa_K510019/annotation2136067/range2136067/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K510019/annotation2136067/range2136067/1
type: Range
persistent identity: range2136067
display id: range2136067
version: 1
top level: BBa_K510019
direction: direction/reverse
start: 2084
end: 2096
orientation: inline

PcI
type: SequenceAnnotation
persistent identity: annotation2136068
display id: annotation2136068
version: 1
top level: BBa_K510019
location: https://synbiohub.org/public/igem/BBa_K510019/annotation2136068/range2136068/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K510019/annotation2136068/range2136068/1
type: Range
persistent identity: range2136068
display id: range2136068
version: 1
top level: BBa_K510019
direction: direction/reverse
start: 2105
end: 2153
orientation: inline

Restriction Site: Sma I
type: SequenceAnnotation
persistent identity: annotation2136069
display id: annotation2136069
version: 1
top level: BBa_K510019
location: https://synbiohub.org/public/igem/BBa_K510019/annotation2136069/range2136069/1
role: SO:0000110
role: feature/dna

https://synbiohub.org/public/igem/BBa_K510019/annotation2136069/range2136069/1
type: Range
persistent identity: range2136069
display id: range2136069
version: 1
top level: BBa_K510019
direction: direction/forward
start: 2171
end: 2176
orientation: inline

PLac
type: SequenceAnnotation
persistent identity: annotation2136070
display id: annotation2136070
version: 1
top level: BBa_K510019
location: https://synbiohub.org/public/igem/BBa_K510019/annotation2136070/range2136070/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K510019/annotation2136070/range2136070/1
type: Range
persistent identity: range2136070
display id: range2136070
version: 1
top level: BBa_K510019
direction: direction/forward
start: 2187
end: 2386
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2136071
display id: annotation2136071
version: 1
top level: BBa_K510019
location: https://synbiohub.org/public/igem/BBa_K510019/annotation2136071/range2136071/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K510019/annotation2136071/range2136071/1
type: Range
persistent identity: range2136071
display id: range2136071
version: 1
top level: BBa_K510019
direction: direction/forward
start: 2395
end: 2407
orientation: inline

cI (Ts)
type: SequenceAnnotation
persistent identity: annotation2136072
display id: annotation2136072
version: 1
top level: BBa_K510019
location: https://synbiohub.org/public/igem/BBa_K510019/annotation2136072/range2136072/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K510019/annotation2136072/range2136072/1
type: Range
persistent identity: range2136072
display id: range2136072
version: 1
top level: BBa_K510019
direction: direction/forward
start: 2414
end: 3127
orientation: inline

Restriction Site: NheI
type: SequenceAnnotation
persistent identity: annotation2136073
display id: annotation2136073
version: 1
top level: BBa_K510019
location: https://synbiohub.org/public/igem/BBa_K510019/annotation2136073/range2136073/1
role: SO:0000110
role: feature/dna

https://synbiohub.org/public/igem/BBa_K510019/annotation2136073/range2136073/1
type: Range
persistent identity: range2136073
display id: range2136073
version: 1
top level: BBa_K510019
direction: direction/forward
start: 3130
end: 3135
orientation: inline

RBS+OmpN::SspB
type: SequenceAnnotation
persistent identity: annotation2136074
display id: annotation2136074
version: 1
top level: BBa_K510019
location: https://synbiohub.org/public/igem/BBa_K510019/annotation2136074/range2136074/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K510019/annotation2136074/range2136074/1
type: Range
persistent identity: range2136074
display id: range2136074
version: 1
top level: BBa_K510019
direction: direction/forward
start: 3139
end: 3732
orientation: inline

RBS+OmpN
type: SequenceAnnotation
persistent identity: annotation2136075
display id: annotation2136075
version: 1
top level: BBa_K510019
location: https://synbiohub.org/public/igem/BBa_K510019/annotation2136075/range2136075/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K510019/annotation2136075/range2136075/1
type: Range
persistent identity: range2136075
display id: range2136075
version: 1
top level: BBa_K510019
direction: direction/forward
start: 3139
end: 3234
orientation: inline

Restriction Site: SacII
type: SequenceAnnotation
persistent identity: annotation2136076
display id: annotation2136076
version: 1
top level: BBa_K510019
location: https://synbiohub.org/public/igem/BBa_K510019/annotation2136076/range2136076/1
role: feature/dna
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K510019/annotation2136076/range2136076/1
type: Range
persistent identity: range2136076
display id: range2136076
version: 1
top level: BBa_K510019
direction: direction/forward
start: 3735
end: 3740
orientation: inline

RBS+OmpN::RFP
type: SequenceAnnotation
persistent identity: annotation2136078
display id: annotation2136078
version: 1
top level: BBa_K510019
location: https://synbiohub.org/public/igem/BBa_K510019/annotation2136078/range2136078/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K510019/annotation2136078/range2136078/1
type: Range
persistent identity: range2136078
display id: range2136078
version: 1
top level: BBa_K510019
direction: direction/forward
start: 3742
end: 4518
orientation: inline

RBS+OmpN
type: SequenceAnnotation
persistent identity: annotation2136077
display id: annotation2136077
version: 1
top level: BBa_K510019
location: https://synbiohub.org/public/igem/BBa_K510019/annotation2136077/range2136077/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K510019/annotation2136077/range2136077/1
type: Range
persistent identity: range2136077
display id: range2136077
version: 1
top level: BBa_K510019
direction: direction/forward
start: 3742
end: 3837
orientation: inline

Restriction Site: StuI
type: SequenceAnnotation
persistent identity: annotation2136079
display id: annotation2136079
version: 1
top level: BBa_K510019
location: https://synbiohub.org/public/igem/BBa_K510019/annotation2136079/range2136079/1
role: feature/dna
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K510019/annotation2136079/range2136079/1
type: Range
persistent identity: range2136079
display id: range2136079
version: 1
top level: BBa_K510019
direction: direction/forward
start: 4526
end: 4531
orientation: inline

Double terminator
type: SequenceAnnotation
persistent identity: annotation2136080
display id: annotation2136080
version: 1
top level: BBa_K510019
location: https://synbiohub.org/public/igem/BBa_K510019/annotation2136080/range2136080/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K510019/annotation2136080/range2136080/1
type: Range
persistent identity: range2136080
display id: range2136080
version: 1
top level: BBa_K510019
direction: direction/forward
start: 4534
end: 4662
orientation: inline

https://synbiohub.org/public/igem/BBa_K510045_sequence/1
type: Sequence
persistent identity: BBa K510045 sequence
display id: BBa_K510045_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactagtagcggccgctgcagtccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaataggccaaccagataagtgaaatctagttccaaactattttgtcatttttaattttcgtattagcttacgacgctacacccagttcccatctattttgtcactcttccctaaataatccttaaaaactccatttccacccctcccagttcccaactattttgtccgcccacaggccgcctaggccgtagcttggcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcatatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagccccgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgagacgaaagggcctcgtgatacgcctatttttataggttaatgtcatgataataatggtttcttagacgtcaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttga…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K510000_sequence/1
type: Sequence
persistent identity: BBa K510000 sequence
display id: BBa_K510000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactagtagcggccgctgcagtccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaataggccaaccagataagtgaaatctagttccaaactattttgtcatttttaattttcgtattagcttacgacgctacacccagttcccatctattttgtcactcttccctaaataatccttaaaaactccatttccacccctcccagttcccaactattttgtccgcccacaggccgcctaggccgtagcttggcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcatatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagccccgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgagacgaaagggcctcgtgatacgcctatttttataggttaatgtcatgataataatggtttcttagacgtcaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttga…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K510019_sequence/1
type: Sequence
persistent identity: BBa K510019 sequence
display id: BBa_K510019_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gagatcatttataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggcttaagtaggcgcgccctattaagaagcatcagcataattttcagagtagttctcgtcgtttgctgctttgtatagttcatccatgccatgtgtaatcccagcagctgttacaaactcaagaaggaccatgtggtctctcttttcgttgggatctttcgaaagggcagattgtgtggacaggtaatggttgtctggtaaaaggacagggccatcgccaattggagtattttgttgataatggtctgctagttgaacgcttccatcttcaatgttgtgtctaattttgaagttaactttgattccattcttttgtttgtctgccatgatgtatacattgtgtgagttatagttgtattccaatttgtgtccaagaatgtttccatcttctttaaaatcaataccttttaactcgattctattaacaagggtatcaccttcaaacttgacttcagcacgtgtcttgtagttcccgtcatctttgaaaaatatagttctttcctgtacataaccttcaggcatggcactcttgaaaaagtcatgctgtttcatatgatctgggtatctcgcaaagcattgaacaccataaccgaaagtagtgacaagtgttggccatggaacaggtagttttccagtagtgcaaataaatttaagggtaagttttccgtatgttgcatcaccttcaccctctccactgacagaaaatttgtgcccattaacatcaccatctaattcaacaagaattgggacaactccagtgaaaagttcttctcctttactcatctagtactttcctgtgtgacggatccactatcaagaagcatcagcataattttcagagtagttctcgtcgtttgctgcctgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgccagggtggtttttcttttcaccagtgagacgggcaacagctgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgccccagcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttcggtatcgtcgtatcccactaccgagatatccgcaccaacgcgcagcccggactcggtaatggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacgatgccctcattcagcatttgcatggtttgttgaaaaccggacatggcactccagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagatatttatgccagccagccagacgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgacccaatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactgttgatgggtgtctggtcagagacatcaagaaataacgccggaacattagtgcaggcagcttccacagcaatggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgttgcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctaccatcgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatcagcaacgactgtttgcccgccagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgcttccacttcttcccgcgttttcgcagaaacgtggctggcc…
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z