LOCUS BBa_K510046 9235 bp DNA linear 31-Dec-2025 DEFINITION pUC18R6KT-miniTn7BB-Gm-improved_flipflop_(module I) ACCESSION BBa_K510046 VERSION BBa_K510046.1 FEATURES Location/Qualifiers misc_feature 2..21 /label=Suffix misc_feature 182..347 /label=Tn7-L end primer_bind 157..176 /label=VR (BBa_G00101) primer binding site CDS 1714..2574 /label=Ampicillin resistance (bla) misc_feature 929..1310 /label=R6K replication origin misc_feature 646..905 /label=oriT misc_feature 348..360 /label=SfiI restriction site misc_feature 3176..3374 /label=Tn7-R end misc_feature 3385..3479 /label=double terminator (BBa_B0014) misc_feature 3490..3537 /label=Flipase recombination sequence (FRT) misc_feature 3163..3175 /label=SfiI restriction site misc_feature 3548..3553 /label=NcoI restriction site CDS 3554..4087 /label=Gentamicin resistance misc_feature 3542..3547 /label=SphI restriction site misc_feature 4345..4350 /label=NcoI restriction site misc_feature 4351..4355 /label=SphI restriction site misc_feature 4528..4549 /label=Prefix primer_bind 4410..4429 /label=VF2 (G00100) primer binding site misc_feature 4357..4404 /label=Flipase recombination sequence (FRT) stem_loop 22..93 /label=E. coli his operon terminator stem_loop 4487..4520 /label=Terminator misc_feature 1..4549 /label=BBa_K510012 misc_feature 4567..4695 /label=Double terminator misc_feature 4696..4701 /label=Restriction Site: AflII misc_feature 4704..4711 /label=Restriction Site: AscI CDS 4712..5476 /label=GFP::Das4 misc_feature 4718..4762 /label=DAS4 RBS 5483..5495 /label=RBS misc_feature 5497..5502 /label=Restriction Site: BamHI CDS 5504..6634 /label=LacI::Das4 misc_feature 5510..5554 /label=DAS4 RBS 6641..6653 /label=RBS promoter 6662..6710 /label=PcI misc_feature 6728..6733 /label=Restriction Site: Sma I promoter 6744..6943 /label=PLac RBS 6952..6964 /label=RBS CDS 6971..7684 /label=cI (Ts) misc_feature 7687..7692 /label=Restriction Site: NheI CDS 7696..8289 /label=RBS+OmpN::SspB misc_feature 7696..7791 /label=RBS+OmpN misc_feature 8292..8297 /label=Restriction Site: SacII misc_feature 8299..8394 /label=RBS+OmpN CDS 8299..9075 /label=RBS+OmpN::RFP misc_feature 9083..9088 /label=Restriction Site: StuI misc_feature 9091..9219 /label=Double terminator misc_feature 4558..9235 /label=BBa_K510019 ORIGIN 1 tactagtagc ggccgctgca gtccggcaaa aaaacgggca aggtgtcacc accctgccct 61 ttttctttaa aaccgaaaag attacttcgc gttatgcagg cttcctcgct cactgactcg 121 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaataggc 181 caaccagata agtgaaatct agttccaaac tattttgtca tttttaattt tcgtattagc 241 ttacgacgct acacccagtt cccatctatt ttgtcactct tccctaaata atccttaaaa 301 actccatttc cacccctccc agttcccaac tattttgtcc gcccacaggc cgcctaggcc 361 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg 421 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc 481 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact 541 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta 601 atacggttat ccacagaatc aggggataac gcaggaaaga acatgttaag gtatactttc 661 cgctgcataa ccctgcttcg gggtcattat agcgattttt tcggtatatc catccttttt 721 cgcacgatat acaggatttt gccaaagggt tcgtgtagac tttccttggt gtatccaacg 781 gcgtcagccg ggcaggatag gtgaagtagg cccacccgcg agcgggtgtt ccttcttcac 841 tgtcccttat tcgcacctgg cggtgctcaa cgggaatcct gctctgcgag gctggccgat 901 aagctctgat aacgcaggaa agaacatggg cagttcaacc tgttgatagt acgtactaag 961 ctctcatgtt tcacgtacta agctctcaag tttaacgtac taagctctca tgtttaacga 1021 actaaaccct catggctaac gtactaagct ctcatggcta acgtactaag ctctcatgtt 1081 tcacgtacta agctctcatg tttgaacaat aaaattaata taaatcagca acttaaatag 1141 cctctaaggt tttaagtttt ataagaaaaa aaagaatata taaggctttt aaagctttta 1201 aggtttaacg gttgtggaca acaagccagg gatgtaacgc actgagaagc ccttagagcc 1261 tctcaaagca attttgagtg acacaggaac acttaacggc tgacaaccgg ctggtaacag 1321 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 1381 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 1441 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt 1501 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 1561 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag 1621 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat 1681 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc 1741 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat 1801 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc 1861 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag 1921 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag 1981 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt 2041 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg 2101 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt 2161 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc 2221 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc 2281 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa 2341 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg 2401 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc 2461 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag 2521 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt 2581 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt 2641 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc 2701 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac 2761 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct 2821 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg 2881 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat 2941 tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata 3001 ccgcatcagg cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg 3061 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg 3121 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccgcct aggcctgtgg 3181 gcggacaata aagtcttaaa ctgaacaaaa tagatctaaa ctatgacaat aaagtcttaa 3241 actagacaga atagttgtaa actgaaatca gtccagttat gctgtgaaaa agcatactgg 3301 acttttgtta tggctaaagc aaactcttca ttttctgaag tgcaaattgc ccgtcgtatt 3361 aaagaggggc gtggggttcg cttgtcacac tggctcacct tcgggtgggc ctttctgcgt 3421 ttatatacta gagagagaat ataaaaagcc agattattaa tccggctttt ttattatttg 3481 ctagatcttg aagtacctat tccgaagttc ctattctcta aaaagtatag gaacttcaga 3541 ggcatgccca tggttaggtg gcggtacttg ggtcgatatc aaagtgcatc acttcttccc 3601 gtatgcccaa ctttgtatag agagccactg cgggatcgtc accgtaatct gcttgcacgt 3661 agatcacata agcaccaagc gcgttggcct catgcttgag gagattgatg agcgcggtgg 3721 caatgccctg cctccggtgc tcgccggaga ctgcgagatc atagatatag atctcactac 3781 gcggctgctc aaacttgggc agaacgtaag ccgcgagagc gccaacaacc gcttcttggt 3841 cgaaggcagc aagcgcgatg aatgtcttac tacggagcaa gttcccgagg taatcggagt 3901 ccggctgatg ttgggagtag gtggctacgt ctccgaactc acgaccgaaa agatcaagag 3961 cagcccgcat ggatttgact tggtcagggc cgagcctaca tgtgcgaatg atgcccatac 4021 ttgagccacc taactttgtt ttagggcgac tgccctgctg cgtaacatcg ttgctgctgc 4081 gtaacatcgt tgctgctcca taacatcaaa catcgaccca cggcgtaacg cgcttgctgc 4141 ttggatgccc gaggcataga ctgtacaaaa aaacagtcat aacaagccat gaaaaccgcc 4201 actgcgccgt taccaccgct gcgttcggtc aaggttctgg accagttgcg tgagcgcata 4261 cgctacttgc attacagttt acgaaccgaa caggcttatg tcaattcgat ccattgctgt 4321 tgacaaaggg aatcagggga tcttccatgg gcatgcgaag tacctattcc gaagttccta 4381 ttctctaaaa agtataggaa cttcagagct gccacctgac gtctaagaaa ccattattat 4441 catgacatta acctataaaa ataggcgtat cacgaggcag aatttcagat aaaaaaaatc 4501 cttagctttc gctaaggatg atttctggaa ttcgcggccg cttctagagt actagaggag 4561 atcatttata aacgcagaaa ggcccacccg aaggtgagcc agtgtgactc tagtagagag 4621 cgttcaccga caaacaacag ataaaacgaa aggcccagtc tttcgactga gcctttcgtt 4681 ttatttgatg cctggcttaa gtaggcgcgc cctattaaga agcatcagca taattttcag 4741 agtagttctc gtcgtttgct gctttgtata gttcatccat gccatgtgta atcccagcag 4801 ctgttacaaa ctcaagaagg accatgtggt ctctcttttc gttgggatct ttcgaaaggg 4861 cagattgtgt ggacaggtaa tggttgtctg gtaaaaggac agggccatcg ccaattggag 4921 tattttgttg ataatggtct gctagttgaa cgcttccatc ttcaatgttg tgtctaattt 4981 tgaagttaac tttgattcca ttcttttgtt tgtctgccat gatgtataca ttgtgtgagt 5041 tatagttgta ttccaatttg tgtccaagaa tgtttccatc ttctttaaaa tcaatacctt 5101 ttaactcgat tctattaaca agggtatcac cttcaaactt gacttcagca cgtgtcttgt 5161 agttcccgtc atctttgaaa aatatagttc tttcctgtac ataaccttca ggcatggcac 5221 tcttgaaaaa gtcatgctgt ttcatatgat ctgggtatct cgcaaagcat tgaacaccat 5281 aaccgaaagt agtgacaagt gttggccatg gaacaggtag ttttccagta gtgcaaataa 5341 atttaagggt aagttttccg tatgttgcat caccttcacc ctctccactg acagaaaatt 5401 tgtgcccatt aacatcacca tctaattcaa caagaattgg gacaactcca gtgaaaagtt 5461 cttctccttt actcatctag tactttcctg tgtgacggat ccactatcaa gaagcatcag 5521 cataattttc agagtagttc tcgtcgtttg ctgcctgccc gctttccagt cgggaaacct 5581 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg 5641 gcgccagggt ggtttttctt ttcaccagtg agacgggcaa cagctgattg cccttcaccg 5701 cctggccctg agagagttgc agcaagcggt ccacgctggt ttgccccagc aggcgaaaat 5761 cctgtttgat ggtggttaac ggcgggatat aacatgagct gtcttcggta tcgtcgtatc 5821 ccactaccga gatatccgca ccaacgcgca gcccggactc ggtaatggcg cgcattgcgc 5881 ccagcgccat ctgatcgttg gcaaccagca tcgcagtggg aacgatgccc tcattcagca 5941 tttgcatggt ttgttgaaaa ccggacatgg cactccagtc gccttcccgt tccgctatcg 6001 gctgaatttg attgcgagtg agatatttat gccagccagc cagacgcaga cgcgccgaga 6061 cagaacttaa tgggcccgct aacagcgcga tttgctggtg acccaatgcg accagatgct 6121 ccacgcccag tcgcgtaccg tcttcatggg agaaaataat actgttgatg ggtgtctggt 6181 cagagacatc aagaaataac gccggaacat tagtgcaggc agcttccaca gcaatggcat 6241 cctggtcatc cagcggatag ttaatgatca gcccactgac gcgttgcgcg agaagattgt 6301 gcaccgccgc tttacaggct tcgacgccgc ttcgttctac catcgacacc accacgctgg 6361 cacccagttg atcggcgcga gatttaatcg ccgcgacaat ttgcgacggc gcgtgcaggg 6421 ccagactgga ggtggcaacg ccaatcagca acgactgttt gcccgccagt tgttgtgcca 6481 cgcggttggg aatgtaattc agctccgcca tcgccgcttc cacttcttcc cgcgttttcg 6541 cagaaacgtg gctggcctgg ttcaccacgc gggaaacggt ctgataagag acaccggcat 6601 actctgcgac atcgtataac gttactggtt tcacctagta ctttcctgtg tgactctagt 6661 agcaaccatt atcaccgcca gaggtaaaat agtcaacacg cacggtgtta agagcgctgg 6721 attactaccc gggacctata ctacaatacg caaaccgcct ctccccgcgc gttggccgat 6781 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc 6841 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc 6901 tcgtatgttg tgtggaattg tgagcggata acaatttcac acatactaga gtcacacagg 6961 aaagtactag atgagcacaa aaaagaaacc attaacacaa gagcagcttg aggacgcacg 7021 tcgccttaaa gcaatttatg aaaaaaagaa aaatgaactt ggcttatccc aggaatctgt 7081 cgcagacaag atggggatgg ggcagtcagg cgttggtgct ttatttaatg gcatcaatgc 7141 attaaatgct tataacgccg cattgcttac aaaaattctc aaagttagcg ttgaagaatt 7201 tagcccttca atcgccagag aaatctacga gatgtatgaa gcggttagta tgcagccgtc 7261 acttagaagt gagtatgagt accctgtttt ttctcatgtt caggcaggga tgttctcacc 7321 tgagcttaga acctttacca aaggtgatgc ggagagatgg gtaagcacaa ccaaaaaagc 7381 cagtgattct gcattctggc ttgaggttga aggtaattcc atgaccgcac caacaggctc 7441 caagccaagc tttcctgacg gaatgttaat tctcgttgac cctgagcagg ctgttgagcc 7501 aggtgatttc tgcatagcca gacttggggg tgatgagttt accttcaaga aactgatcag 7561 ggatagcggt caggtgtttt tacaaccact aaacccacag tacccaatga tcccatgcaa 7621 tgagagttgt tccgttgtgg ggaaagttat cgctagtcag tggcctgaag agacgtttgg 7681 ctgatagcta gcaggtagga tgcctttgat tcaacgaatc tgtagaagtt caatcttttg 7741 caaataagtt aagtttttaa ggataaaaaa atgaaaagaa aagtattggc aatggatttg 7801 tcacagctaa caccacgtcg tccctatctg ctgcgtgcat tctatgagtg gttgctggat 7861 aaccagctca cgccgcacct ggtggtggat gtgacgctcc caggcgtgca ggttcctatg 7921 gaatatgcgc gtgacgggca aatcgtactc aacattgcgc cgcgtgctgt cggcaatctg 7981 gaactggcga atgatgaggt gcgctttaac gcgcgctttg gtggcattcc gcgtcaggtt 8041 tctgtgccgc tggctgccgt gctggctatc tacgcccgtg aaaatggtgc aggcacgatg 8101 tttgagcctg aagctgccta cgatgaagat gccagcatca tgaatgatga agaggcatcg 8161 gcagacaacg aaaccgttat gtcggttatc gatggcgaca agccagatca cgatgatgaa 8221 actcatcctg acgatgaacc tccgcagcca ccacgcggtg gtcgaccggc attacgcgtt 8281 gtgaagtaat accgcgggta ggatgccttt gattcaacga atctgtagaa gttcaatctt 8341 ttgcaaataa gttaagtttt taaggataaa aaaatgaaaa gaaaagtatt ggcaatggct 8401 tcctccgaag acgttatcaa agagttcatg cgtttcaaag ttcgtatgga aggttccgtt 8461 aacggtcacg agttcgaaat cgaaggtgaa ggtgaaggtc gtccgtacga aggtacccag 8521 accgctaaac tgaaagttac caaaggtggt ccgctgccgt tcgcttggga catcctgtcc 8581 ccgcagttcc agtacggttc caaagcttac gttaaacacc cggctgacat cccggactac 8641 ctgaaactgt ccttcccgga aggtttcaaa tgggaacgtg ttatgaactt cgaagacggt 8701 ggtgttgtta ccgttaccca ggactcctcc ctgcaagacg gtgagttcat ctacaaagtt 8761 aaactgcgtg gtaccaactt cccgtccgac ggtccggtta tgcagaaaaa aaccatgggt 8821 tgggaagctt ccaccgaacg tatgtacccg gaagacggtg ctctgaaagg tgaaatcaaa 8881 atgcgtctga aactgaaaga cggtggtcac tacgacgctg aagttaaaac cacctacatg 8941 gctaaaaaac cggttcagct gccgggtgct tacaaaaccg acatcaaact ggacatcacc 9001 tcccacaacg aagactacac catcgttgaa cagtacgaac gtgctgaagg tcgtcactcc 9061 accggtgctt aataatgata ctaggcctag ccaggcatca aataaaacga aaggctcagt 9121 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc 9181 acactggctc accttcgggt gggcctttct gcgtttatat actagagtac tagag //