##gff-version 3
##sequence-region BBa_K510046 1 9235
BBa_K510046	.	engineered_region	4558	9235	.	+	0	ID=BBa_K510019;Name=BBa_K510019
BBa_K510046	.	sequence_feature	5497	5502	.	+	0	ID=annotation2136064;Name=Restriction Site: BamHI;Parent=BBa_K510019
BBa_K510046	.	stop_codon	9091	9219	.	+	0	ID=annotation2136080;Name=Double terminator;Parent=BBa_K510019
BBa_K510046	.	sequence_feature	4696	4701	.	+	0	ID=annotation2136059;Name=Restriction Site: AflII;Parent=BBa_K510019
BBa_K510046	.	CDS	5504	6634	.	+	0	ID=annotation2136065;Name=LacI::Das4;Parent=BBa_K510019
BBa_K510046	.	CDS	6971	7684	.	+	0	ID=annotation2136072;Name=cI (Ts);Parent=BBa_K510019
BBa_K510046	.	CDS	7696	8289	.	+	0	ID=annotation2136074;Name=RBS+OmpN::SspB;Parent=BBa_K510019
BBa_K510046	.	sequence_feature	4704	4711	.	+	0	ID=annotation2136060;Name=Restriction Site: AscI;Parent=BBa_K510019
BBa_K510046	.	tag	4718	4762	.	+	0	ID=annotation2136062;Name=DAS4;Parent=BBa_K510019
BBa_K510046	.	sequence_feature	9083	9088	.	+	0	ID=annotation2136079;Name=Restriction Site: StuI;Parent=BBa_K510019
BBa_K510046	.	CDS	8299	9075	.	+	0	ID=annotation2136078;Name=RBS+OmpN::RFP;Parent=BBa_K510019
BBa_K510046	.	stop_codon	4567	4695	.	+	0	ID=annotation2136058;Name=Double terminator;Parent=BBa_K510019
BBa_K510046	.	tag	5510	5554	.	+	0	ID=annotation2136066;Name=DAS4;Parent=BBa_K510019
BBa_K510046	.	promoter	6744	6943	.	+	0	ID=annotation2136070;Name=PLac;Parent=BBa_K510019
BBa_K510046	.	sequence_feature	8292	8297	.	+	0	ID=annotation2136076;Name=Restriction Site: SacII;Parent=BBa_K510019
BBa_K510046	.	sequence_feature	8299	8394	.	+	0	ID=annotation2136077;Name=RBS+OmpN;Parent=BBa_K510019
BBa_K510046	.	ribosome_entry_site	6641	6653	.	+	0	ID=annotation2136067;Name=RBS;Parent=BBa_K510019
BBa_K510046	.	sequence_feature	7696	7791	.	+	0	ID=annotation2136075;Name=RBS+OmpN;Parent=BBa_K510019
BBa_K510046	.	sequence_feature	7687	7692	.	+	0	ID=annotation2136073;Name=Restriction Site: NheI;Parent=BBa_K510019
BBa_K510046	.	CDS	4712	5476	.	+	0	ID=annotation2136061;Name=GFP::Das4;Parent=BBa_K510019
BBa_K510046	.	ribosome_entry_site	5483	5495	.	+	0	ID=annotation2136063;Name=RBS;Parent=BBa_K510019
BBa_K510046	.	sequence_feature	6728	6733	.	+	0	ID=annotation2136069;Name=Restriction Site: Sma I;Parent=BBa_K510019
BBa_K510046	.	promoter	6662	6710	.	+	0	ID=annotation2136068;Name=PcI;Parent=BBa_K510019
BBa_K510046	.	ribosome_entry_site	6952	6964	.	+	0	ID=annotation2136071;Name=RBS;Parent=BBa_K510019
BBa_K510046	.	plasmid	1	4549	.	+	0	ID=BBa_K510012;Name=BBa_K510012
BBa_K510046	.	sequence_feature	3548	3553	.	+	0	ID=annotation2131804;Name=NcoI restriction site;Parent=BBa_K510012
BBa_K510046	.	sequence_feature	3490	3537	.	+	0	ID=annotation2131802;Name=Flipase recombination sequence (FRT);Parent=BBa_K510012
BBa_K510046	.	engineered_region	2	21	.	+	0	ID=annotation2131765;Name=Suffix;Parent=BBa_K510012
BBa_K510046	.	sequence_feature	348	360	.	+	0	ID=annotation2131798;Name=SfiI restriction site;Parent=BBa_K510012
BBa_K510046	.	sequence_feature	3542	3547	.	+	0	ID=annotation2131806;Name=SphI restriction site;Parent=BBa_K510012
BBa_K510046	.	sequence_feature	4351	4355	.	+	0	ID=annotation2131808;Name=SphI restriction site;Parent=BBa_K510012
BBa_K510046	.	primer_binding_site	157	176	.	+	0	ID=annotation2131786;Name=VR (BBa_G00101) primer binding site;Parent=BBa_K510012
BBa_K510046	.	primer_binding_site	4410	4429	.	+	0	ID=annotation2131841;Name=VF2 (G00100) primer binding site;Parent=BBa_K510012
BBa_K510046	.	sequence_feature	646	905	.	+	0	ID=annotation2131797;Name=oriT;Parent=BBa_K510012
BBa_K510046	.	engineered_region	4528	4549	.	+	0	ID=annotation2131839;Name=Prefix;Parent=BBa_K510012
BBa_K510046	.	sequence_feature	4357	4404	.	+	0	ID=annotation2131856;Name=Flipase recombination sequence (FRT);Parent=BBa_K510012
BBa_K510046	.	stem_loop	22	93	.	+	0	ID=annotation2131863;Name=E. coli his operon terminator;Parent=BBa_K510012
BBa_K510046	.	sequence_feature	4345	4350	.	+	0	ID=annotation2131807;Name=NcoI restriction site;Parent=BBa_K510012
BBa_K510046	.	sequence_feature	929	1310	.	+	0	ID=annotation2131796;Name=R6K replication origin;Parent=BBa_K510012
BBa_K510046	.	sequence_feature	3163	3175	.	+	0	ID=annotation2131803;Name=SfiI restriction site;Parent=BBa_K510012
BBa_K510046	.	engineered_region	3385	3479	.	+	0	ID=annotation2131801;Name=double terminator (BBa_B0014);Parent=BBa_K510012
BBa_K510046	.	CDS	3554	4087	.	+	0	ID=annotation2131805;Name=Gentamicin resistance;Parent=BBa_K510012
BBa_K510046	.	stem_loop	4487	4520	.	+	0	ID=annotation2131866;Name=Terminator;Parent=BBa_K510012
BBa_K510046	.	sequence_feature	3176	3374	.	+	0	ID=annotation2131800;Name=Tn7-R end;Parent=BBa_K510012
BBa_K510046	.	sequence_feature	182	347	.	+	0	ID=annotation2131766;Name=Tn7-L end;Parent=BBa_K510012
BBa_K510046	.	CDS	1714	2574	.	+	0	ID=annotation2131795;Name=Ampicillin resistance (bla);Parent=BBa_K510012
>BBa_K510046
tactagtagcggccgctgcagtccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaa
gattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagc
tcactcaaaggcggtaataggccaaccagataagtgaaatctagttccaaactattttgtcatttttaattttcgtatt
agcttacgacgctacacccagttcccatctattttgtcactcttccctaaataatccttaaaaactccatttccacccc
tcccagttcccaactattttgtccgcccacaggccgcctaggccggaagcataaagtgtaaagcctggggtgcctaatg
agtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaa
tgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgct
cggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgc
aggaaagaacatgttaaggtatactttccgctgcataaccctgcttcggggtcattatagcgattttttcggtatatcc
atcctttttcgcacgatatacaggattttgccaaagggttcgtgtagactttccttggtgtatccaacggcgtcagccg
ggcaggataggtgaagtaggcccacccgcgagcgggtgttccttcttcactgtcccttattcgcacctggcggtgctca
acgggaatcctgctctgcgaggctggccgataagctctgataacgcaggaaagaacatgggcagttcaacctgttgata
gtacgtactaagctctcatgtttcacgtactaagctctcaagtttaacgtactaagctctcatgtttaacgaactaaac
cctcatggctaacgtactaagctctcatggctaacgtactaagctctcatgtttcacgtactaagctctcatgtttgaa
caataaaattaatataaatcagcaacttaaatagcctctaaggttttaagttttataagaaaaaaaagaatatataagg
cttttaaagcttttaaggtttaacggttgtggacaacaagccagggatgtaacgcactgagaagcccttagagcctctc
aaagcaattttgagtgacacaggaacacttaacggctgacaaccggctggtaacaggattagcagagcgaggtatgtag
gcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgct
gaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggttttttt
gtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctc
agtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaatta
aaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggca
cctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagg
gcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaacca
gccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaa
gctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgt
cgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagc
ggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactg
cataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaat
agtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagt
gctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaaccc
actcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatg
ccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcattta
tcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacattt
ccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggc
cctttcgtctcgcgcgtttcggtgatgacggtgaaaacctctgacacatgcagctcccggagacggtcacagcttgtct
gtaagcggatgccgggagcagacaagcccgtcagggcgcgtcagcgggtgttggcgggtgtcggggctggcttaactat
gcggcatcagagcagattgtactgagagtgcaccatatgcggtgtgaaataccgcacagatgcgtaaggagaaaatacc
gcatcaggcgccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggcctcttcgctattacgcca
gctggcgaaagggggatgtgctgcaaggcgattaagttgggtaacgccagggttttcccagtcacgacgttgtaaaacg
acggccgcctaggcctgtgggcggacaataaagtcttaaactgaacaaaatagatctaaactatgacaataaagtctta
aactagacagaatagttgtaaactgaaatcagtccagttatgctgtgaaaaagcatactggacttttgttatggctaaa
gcaaactcttcattttctgaagtgcaaattgcccgtcgtattaaagaggggcgtggggttcgcttgtcacactggctca
ccttcgggtgggcctttctgcgtttatatactagagagagaatataaaaagccagattattaatccggcttttttatta
tttgctagatcttgaagtacctattccgaagttcctattctctaaaaagtataggaacttcagaggcatgcccatggtt
aggtggcggtacttgggtcgatatcaaagtgcatcacttcttcccgtatgcccaactttgtatagagagccactgcggg
atcgtcaccgtaatctgcttgcacgtagatcacataagcaccaagcgcgttggcctcatgcttgaggagattgatgagc
gcggtggcaatgccctgcctccggtgctcgccggagactgcgagatcatagatatagatctcactacgcggctgctcaa
acttgggcagaacgtaagccgcgagagcgccaacaaccgcttcttggtcgaaggcagcaagcgcgatgaatgtcttact
acggagcaagttcccgaggtaatcggagtccggctgatgttgggagtaggtggctacgtctccgaactcacgaccgaaa
agatcaagagcagcccgcatggatttgacttggtcagggccgagcctacatgtgcgaatgatgcccatacttgagccac
ctaactttgttttagggcgactgccctgctgcgtaacatcgttgctgctgcgtaacatcgttgctgctccataacatca
aacatcgacccacggcgtaacgcgcttgctgcttggatgcccgaggcatagactgtacaaaaaaacagtcataacaagc
catgaaaaccgccactgcgccgttaccaccgctgcgttcggtcaaggttctggaccagttgcgtgagcgcatacgctac
ttgcattacagtttacgaaccgaacaggcttatgtcaattcgatccattgctgttgacaaagggaatcaggggatcttc
catgggcatgcgaagtacctattccgaagttcctattctctaaaaagtataggaacttcagagctgccacctgacgtct
aagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatcctt
agctttcgctaaggatgatttctggaattcgcggccgcttctagagtactagaggagatcatttataaacgcagaaagg
cccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaaacgaaaggcccagtct
ttcgactgagcctttcgttttatttgatgcctggcttaagtaggcgcgccctattaagaagcatcagcataattttcag
agtagttctcgtcgtttgctgctttgtatagttcatccatgccatgtgtaatcccagcagctgttacaaactcaagaag
gaccatgtggtctctcttttcgttgggatctttcgaaagggcagattgtgtggacaggtaatggttgtctggtaaaagg
acagggccatcgccaattggagtattttgttgataatggtctgctagttgaacgcttccatcttcaatgttgtgtctaa
ttttgaagttaactttgattccattcttttgtttgtctgccatgatgtatacattgtgtgagttatagttgtattccaa
tttgtgtccaagaatgtttccatcttctttaaaatcaataccttttaactcgattctattaacaagggtatcaccttca
aacttgacttcagcacgtgtcttgtagttcccgtcatctttgaaaaatatagttctttcctgtacataaccttcaggca
tggcactcttgaaaaagtcatgctgtttcatatgatctgggtatctcgcaaagcattgaacaccataaccgaaagtagt
gacaagtgttggccatggaacaggtagttttccagtagtgcaaataaatttaagggtaagttttccgtatgttgcatca
ccttcaccctctccactgacagaaaatttgtgcccattaacatcaccatctaattcaacaagaattgggacaactccag
tgaaaagttcttctcctttactcatctagtactttcctgtgtgacggatccactatcaagaagcatcagcataattttc
agagtagttctcgtcgtttgctgcctgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggcc
aacgcgcggggagaggcggtttgcgtattgggcgccagggtggtttttcttttcaccagtgagacgggcaacagctgat
tgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgccccagcaggcgaaaatcctgttt
gatggtggttaacggcgggatataacatgagctgtcttcggtatcgtcgtatcccactaccgagatatccgcaccaacg
cgcagcccggactcggtaatggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacga
tgccctcattcagcatttgcatggtttgttgaaaaccggacatggcactccagtcgccttcccgttccgctatcggctg
aatttgattgcgagtgagatatttatgccagccagccagacgcagacgcgccgagacagaacttaatgggcccgctaac
agcgcgatttgctggtgacccaatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaaataatac
tgttgatgggtgtctggtcagagacatcaagaaataacgccggaacattagtgcaggcagcttccacagcaatggcatc
ctggtcatccagcggatagttaatgatcagcccactgacgcgttgcgcgagaagattgtgcaccgccgctttacaggct
tcgacgccgcttcgttctaccatcgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaa
tttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatcagcaacgactgtttgcccgccagttgttgtgc
cacgcggttgggaatgtaattcagctccgccatcgccgcttccacttcttcccgcgttttcgcagaaacgtggctggcc
tggttcaccacgcgggaaacggtctgataagagacaccggcatactctgcgacatcgtataacgttactggtttcacct
agtactttcctgtgtgactctagtagcaaccattatcaccgccagaggtaaaatagtcaacacgcacggtgttaagagc
gctggattactacccgggacctatactacaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagct
ggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcacc
ccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagt
cacacaggaaagtactagatgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaag
caatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcagg
cgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttacaaaaattctcaaagttagc
gttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaa
gtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtga
tgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgacc
gcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtg
atttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttaca
accactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtgg
cctgaagagacgtttggctgatagctagcaggtaggatgcctttgattcaacgaatctgtagaagttcaatcttttgca
aataagttaagtttttaaggataaaaaaatgaaaagaaaagtattggcaatggatttgtcacagctaacaccacgtcgt
ccctatctgctgcgtgcattctatgagtggttgctggataaccagctcacgccgcacctggtggtggatgtgacgctcc
caggcgtgcaggttcctatggaatatgcgcgtgacgggcaaatcgtactcaacattgcgccgcgtgctgtcggcaatct
ggaactggcgaatgatgaggtgcgctttaacgcgcgctttggtggcattccgcgtcaggtttctgtgccgctggctgcc
gtgctggctatctacgcccgtgaaaatggtgcaggcacgatgtttgagcctgaagctgcctacgatgaagatgccagca
tcatgaatgatgaagaggcatcggcagacaacgaaaccgttatgtcggttatcgatggcgacaagccagatcacgatga
tgaaactcatcctgacgatgaacctccgcagccaccacgcggtggtcgaccggcattacgcgttgtgaagtaataccgc
gggtaggatgcctttgattcaacgaatctgtagaagttcaatcttttgcaaataagttaagtttttaaggataaaaaaa
tgaaaagaaaagtattggcaatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaagg
ttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactg
aaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacg
ttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaactt
cgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgt
ggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacc
cggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaa
aaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccac
aacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataatgatactagg
cctagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaac
gctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtactagag