BBa_K510046
type: ComponentDefinition
persistent identity: BBa K510046
display id: BBa_K510046
version: 1
was generated by: iGEM to SBOL conversion
description: pUC18R6KT-miniTn7BB-Gm-improved_flipflop_(module I)
created: 2011-10-21T11:00:00Z
modified: 2015-05-08T01:12:30Z
mutable provenance: The pUC18R6KT vector was amplified by PCR using as template a pTNS2 plasmid (GenBank accession number: AY884833) provided by Herbert P. Schweizer. The primers were designed to flanked the pUC18R6KT with SfiI restriction sites and allow the insertion of the mini-Tn7-Gm. Primers: attcGGCCTAGGCGGCCgtcgttttacaacgtcgtgac and attcGGCCGCCTAGGCCggaagcataaagtgtaaagcctg. The miniTn7BB-Gm minitransposon was synthesized commercially, and then digested at the flanking SfiI sites and cloned into SfiI-digested pUC18R6KT PCR products. The improved flip-flop (module I) was synthesized commercially too.
owned by: james
owned by: myers
mutable description: This plasmid allows the integration of the improved flip-flop (module I) (part: BBa_K51019) into bacterial chromosomes using the pUC18Sfi-miniTn7BB-Gm (BBa_K51012) characteristics. Single copy may improve the function of regulatory circuits, as bistable systems. For more information about the function of the miniTn7 BioBrick toolkit and flip-flops, visit the iGEM team UPO-Sevilla 2011 wiki.
discontinued: false
dominant: false
experience: experience/None
group u list: _673_
m user id: 0
owner id: 6163
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: The BBa_K510019 BioBrick was inserted within the BCS of pUC18R6KT-miniTn7BB-Gm (BBa K510012) by EcoRI and PstI clonning.
star: false
creator: David Caballero, Felix Reyes
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K510012
component: BBa_K510019
sequence annotation: BBa_K510012
sequence annotation: BBa_K510019
sequence: https://synbiohub.org/public/igem/BBa_K510046_sequence/1

BBa_K510012
type: Component
persistent identity: component2160083
display id: component2160083
version: 1
top level: BBa_K510046
definition: BBa_K510012
access: public

BBa_K510019
type: Component
persistent identity: component2160107
display id: component2160107
version: 1
top level: BBa_K510046
definition: BBa_K510019
access: public

BBa_K510012
type: SequenceAnnotation
persistent identity: annotation2160083
display id: annotation2160083
version: 1
top level: BBa_K510046
location: https://synbiohub.org/public/igem/BBa_K510046/annotation2160083/range2160083/1
component: BBa_K510012

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type: Range
persistent identity: range2160083
display id: range2160083
version: 1
top level: BBa_K510046
direction: direction/reverse
start: 1
end: 4549
orientation: inline

BBa_K510019
type: SequenceAnnotation
persistent identity: annotation2160107
display id: annotation2160107
version: 1
top level: BBa_K510046
location: https://synbiohub.org/public/igem/BBa_K510046/annotation2160107/range2160107/1
component: BBa_K510019

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type: Range
persistent identity: range2160107
display id: range2160107
version: 1
top level: BBa_K510046
direction: direction/reverse
start: 4558
end: 9235
orientation: inline

BBa_K510012
type: ComponentDefinition
persistent identity: BBa K510012
display id: BBa_K510012
version: 1
was generated by: iGEM to SBOL conversion
description: pUC18R6KT-miniTn7BB-Gm
created: 2011-09-17T11:00:00Z
modified: 2015-05-08T01:12:30Z
mutable provenance: The pUC18R6KT vector was amplified by PCR using as template a pTNS2 plasmid (GenBank accession number: AY884833) provided by Herbert P. Schweizer. The primers were designed to flanked the pUC18R6KT with SfiI restriction sites and allow the insertion of the mini-Tn7-Gm. Primers: attcGGCCTAGGCGGCCgtcgttttacaacgtcgtgac and attcGGCCGCCTAGGCCggaagcataaagtgtaaagcctg. The miniTn7BB-Gm minitransposon was synthesized commercially, and then digested at the flanking SfiI sites and cloned into SfiI-digested pUC18R6KT PCR products.
owned by: james
owned by: myers
mutable description: pUC18R6KT-miniTn7BB-Gm is a vehicle vector for the minitransposon miniTn7BB-Gm. This transposon is a part of the miniTn7 BioBrick toolkit, a set of plasmids harboring Tn7 derivatives that can be used for integration of BioBricks in single copy at a conserved target (the attTn7 site), in multiple bacteria. The structure of the mini-Tn7BB-Gm transposon is shown in the figure below. Tn7R and Tn7L are the right and left ends of Tn7 transposon, respectively, required for recognition of the transposase machinery and transposition. FRT is the Flp recombinase target site for excision of the resistance once the transposon is inserted in the genome of a living organism. The gentamycin resistance cassette (Gm) is flanked by restriction sites in order to facilitate the generation of variants with different antibiotic resistance markers. The BioBrick cloning site (BCS) includes the prefix and suffix restrictions sites used in assembly standard 10, and it is the place where BioBricks are inserted for genome integration. This plasmid is replicative only in E. coli strains expressing the pir gene thanks to its R6K replication origin. Also it has an origin for transference (oriT) for conjugation purposes. This plasmid can be used to maintain the miniTn7-Gm transposon in E. coli pir+ strains and may be used for transposition into the genomes of any bacteria without the pir gen, in which it is non-replicative. As a BioBrick cloning vector, pUC18R6KT-miniTn7BB-Gm is fully compatible with Assembly Standard 10. The complete transposon is flanked by SfiI restriction sites to facilitate subcloning in other vectors.
discontinued: false
dominant: false
experience: experience/Works
group u list: _673_
m user id: 0
owner id: 6163
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: Kyoung-Hee Choi, Jared B Gaynor, Kimberly G White, Carolina Lopez, Catharine M Bosio, RoxAnn R Karkhoff-Schweizer & Herbert P Schweizer (2005). A Tn7-based broad-range bacterial cloning and expression system. Nature Methods, vol.2 NO.6, June 2005, 443.
star: false
creator: David Caballero & Fernando Govantes
type: DnaRegion
role: SO:0000155
role: partType/Plasmid
sequence annotation: Suffix
sequence annotation: E. coli his operon terminator
sequence annotation: VR (BBa_G00101) primer binding site
sequence annotation: Tn7-L end
sequence annotation: SfiI restriction site
sequence annotation: oriT
sequence annotation: R6K replication origin
sequence annotation: Ampicillin resistance (bla)
sequence annotation: Tn7-R end
sequence annotation: double terminator (BBa_B0014)
sequence annotation: Flipase recombination sequence (FRT)
sequence annotation: SphI restriction site
sequence annotation: NcoI restriction site
sequence annotation: Gentamicin resistance
sequence annotation: VF2 (G00100) primer binding site
sequence annotation: Terminator
sequence annotation: Prefix
sequence: https://synbiohub.org/public/igem/BBa_K510012_sequence/1

Suffix
type: SequenceAnnotation
persistent identity: annotation2131765
display id: annotation2131765
version: 1
top level: BBa_K510012
location: https://synbiohub.org/public/igem/BBa_K510012/annotation2131765/range2131765/1
role: SO:0000804
role: feature/BioBrick

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type: Range
persistent identity: range2131765
display id: range2131765
version: 1
top level: BBa_K510012
direction: direction/forward
start: 2
end: 21
orientation: inline

E. coli his operon terminator
type: SequenceAnnotation
persistent identity: annotation2131863
display id: annotation2131863
version: 1
top level: BBa_K510012
location: https://synbiohub.org/public/igem/BBa_K510012/annotation2131863/range2131863/1
role: feature/stem loop
role: SO:0000313

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type: Range
persistent identity: range2131863
display id: range2131863
version: 1
top level: BBa_K510012
direction: direction/forward
start: 22
end: 93
orientation: inline

VR (BBa_G00101) primer binding site
type: SequenceAnnotation
persistent identity: annotation2131786
display id: annotation2131786
version: 1
top level: BBa_K510012
location: https://synbiohub.org/public/igem/BBa_K510012/annotation2131786/range2131786/1
role: SO:0005850
role: feature/primer binding

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type: Range
persistent identity: range2131786
display id: range2131786
version: 1
top level: BBa_K510012
direction: direction/reverse
start: 157
end: 176
orientation: inline

Tn7-L end
type: SequenceAnnotation
persistent identity: annotation2131766
display id: annotation2131766
version: 1
top level: BBa_K510012
location: https://synbiohub.org/public/igem/BBa_K510012/annotation2131766/range2131766/1
role: SO:0000110
role: feature/misc

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type: Range
persistent identity: range2131766
display id: range2131766
version: 1
top level: BBa_K510012
direction: direction/forward
start: 182
end: 347
orientation: inline

SfiI restriction site
type: SequenceAnnotation
persistent identity: annotation2131798
display id: annotation2131798
version: 1
top level: BBa_K510012
location: https://synbiohub.org/public/igem/BBa_K510012/annotation2131798/range2131798/1
role: feature/dna
role: SO:0000110

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type: Range
persistent identity: range2131798
display id: range2131798
version: 1
top level: BBa_K510012
direction: direction/forward
start: 348
end: 360
orientation: inline

oriT
type: SequenceAnnotation
persistent identity: annotation2131797
display id: annotation2131797
version: 1
top level: BBa_K510012
location: https://synbiohub.org/public/igem/BBa_K510012/annotation2131797/range2131797/1
role: feature/dna
role: SO:0000110

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type: Range
persistent identity: range2131797
display id: range2131797
version: 1
top level: BBa_K510012
direction: direction/forward
start: 646
end: 905
orientation: inline

R6K replication origin
type: SequenceAnnotation
persistent identity: annotation2131796
display id: annotation2131796
version: 1
top level: BBa_K510012
location: https://synbiohub.org/public/igem/BBa_K510012/annotation2131796/range2131796/1
role: feature/dna
role: SO:0000110

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type: Range
persistent identity: range2131796
display id: range2131796
version: 1
top level: BBa_K510012
direction: direction/forward
start: 929
end: 1310
orientation: inline

Ampicillin resistance (bla)
type: SequenceAnnotation
persistent identity: annotation2131795
display id: annotation2131795
version: 1
top level: BBa_K510012
location: https://synbiohub.org/public/igem/BBa_K510012/annotation2131795/range2131795/1
role: feature/cds
role: SO:0000316

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type: Range
persistent identity: range2131795
display id: range2131795
version: 1
top level: BBa_K510012
direction: direction/reverse
start: 1714
end: 2574
orientation: inline

SfiI restriction site
type: SequenceAnnotation
persistent identity: annotation2131803
display id: annotation2131803
version: 1
top level: BBa_K510012
location: https://synbiohub.org/public/igem/BBa_K510012/annotation2131803/range2131803/1
role: feature/dna
role: SO:0000110

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type: Range
persistent identity: range2131803
display id: range2131803
version: 1
top level: BBa_K510012
direction: direction/forward
start: 3163
end: 3175
orientation: inline

Tn7-R end
type: SequenceAnnotation
persistent identity: annotation2131800
display id: annotation2131800
version: 1
top level: BBa_K510012
location: https://synbiohub.org/public/igem/BBa_K510012/annotation2131800/range2131800/1
role: feature/misc
role: SO:0000110

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type: Range
persistent identity: range2131800
display id: range2131800
version: 1
top level: BBa_K510012
direction: direction/forward
start: 3176
end: 3374
orientation: inline

double terminator (BBa_B0014)
type: SequenceAnnotation
persistent identity: annotation2131801
display id: annotation2131801
version: 1
top level: BBa_K510012
location: https://synbiohub.org/public/igem/BBa_K510012/annotation2131801/range2131801/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K510012/annotation2131801/range2131801/1
type: Range
persistent identity: range2131801
display id: range2131801
version: 1
top level: BBa_K510012
direction: direction/forward
start: 3385
end: 3479
orientation: inline

Flipase recombination sequence (FRT)
type: SequenceAnnotation
persistent identity: annotation2131802
display id: annotation2131802
version: 1
top level: BBa_K510012
location: https://synbiohub.org/public/igem/BBa_K510012/annotation2131802/range2131802/1
role: feature/dna
role: SO:0000110

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type: Range
persistent identity: range2131802
display id: range2131802
version: 1
top level: BBa_K510012
direction: direction/forward
start: 3490
end: 3537
orientation: inline

SphI restriction site
type: SequenceAnnotation
persistent identity: annotation2131806
display id: annotation2131806
version: 1
top level: BBa_K510012
location: https://synbiohub.org/public/igem/BBa_K510012/annotation2131806/range2131806/1
role: feature/dna
role: SO:0000110

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type: Range
persistent identity: range2131806
display id: range2131806
version: 1
top level: BBa_K510012
direction: direction/forward
start: 3542
end: 3547
orientation: inline

NcoI restriction site
type: SequenceAnnotation
persistent identity: annotation2131804
display id: annotation2131804
version: 1
top level: BBa_K510012
location: https://synbiohub.org/public/igem/BBa_K510012/annotation2131804/range2131804/1
role: feature/dna
role: SO:0000110

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type: Range
persistent identity: range2131804
display id: range2131804
version: 1
top level: BBa_K510012
direction: direction/forward
start: 3548
end: 3553
orientation: inline

Gentamicin resistance
type: SequenceAnnotation
persistent identity: annotation2131805
display id: annotation2131805
version: 1
top level: BBa_K510012
location: https://synbiohub.org/public/igem/BBa_K510012/annotation2131805/range2131805/1
role: SO:0000316
role: feature/cds

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type: Range
persistent identity: range2131805
display id: range2131805
version: 1
top level: BBa_K510012
direction: direction/reverse
start: 3554
end: 4087
orientation: inline

NcoI restriction site
type: SequenceAnnotation
persistent identity: annotation2131807
display id: annotation2131807
version: 1
top level: BBa_K510012
location: https://synbiohub.org/public/igem/BBa_K510012/annotation2131807/range2131807/1
role: feature/dna
role: SO:0000110

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type: Range
persistent identity: range2131807
display id: range2131807
version: 1
top level: BBa_K510012
direction: direction/forward
start: 4345
end: 4350
orientation: inline

SphI restriction site
type: SequenceAnnotation
persistent identity: annotation2131808
display id: annotation2131808
version: 1
top level: BBa_K510012
location: https://synbiohub.org/public/igem/BBa_K510012/annotation2131808/range2131808/1
role: SO:0000110
role: feature/dna

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type: Range
persistent identity: range2131808
display id: range2131808
version: 1
top level: BBa_K510012
direction: direction/forward
start: 4351
end: 4355
orientation: inline

Flipase recombination sequence (FRT)
type: SequenceAnnotation
persistent identity: annotation2131856
display id: annotation2131856
version: 1
top level: BBa_K510012
location: https://synbiohub.org/public/igem/BBa_K510012/annotation2131856/range2131856/1
role: SO:0000110
role: feature/dna

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type: Range
persistent identity: range2131856
display id: range2131856
version: 1
top level: BBa_K510012
direction: direction/forward
start: 4357
end: 4404
orientation: inline

VF2 (G00100) primer binding site
type: SequenceAnnotation
persistent identity: annotation2131841
display id: annotation2131841
version: 1
top level: BBa_K510012
location: https://synbiohub.org/public/igem/BBa_K510012/annotation2131841/range2131841/1
role: SO:0005850
role: feature/primer binding

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type: Range
persistent identity: range2131841
display id: range2131841
version: 1
top level: BBa_K510012
direction: direction/forward
start: 4410
end: 4429
orientation: inline

Terminator
type: SequenceAnnotation
persistent identity: annotation2131866
display id: annotation2131866
version: 1
top level: BBa_K510012
location: https://synbiohub.org/public/igem/BBa_K510012/annotation2131866/range2131866/1
role: feature/stem loop
role: SO:0000313

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type: Range
persistent identity: range2131866
display id: range2131866
version: 1
top level: BBa_K510012
direction: direction/forward
start: 4487
end: 4520
orientation: inline

Prefix
type: SequenceAnnotation
persistent identity: annotation2131839
display id: annotation2131839
version: 1
top level: BBa_K510012
location: https://synbiohub.org/public/igem/BBa_K510012/annotation2131839/range2131839/1
role: SO:0000804
role: feature/BioBrick

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type: Range
persistent identity: range2131839
display id: range2131839
version: 1
top level: BBa_K510012
direction: direction/forward
start: 4528
end: 4549
orientation: inline

BBa_K510019
type: ComponentDefinition
persistent identity: BBa K510019
display id: BBa_K510019
version: 1
was generated by: iGEM to SBOL conversion
description: Improved Flip Flop (Module I)
created: 2011-09-18T11:00:00Z
modified: 2015-05-08T01:12:30Z
mutable provenance: It's a part which comes from different genes forming the bassic flip-flop with anothers improvements.
owned by: james
owned by: myers
mutable description: Here we show the final structure of our improved bistable. As well as the two operons with their protein fusions and the promoter with the asRNA, we show the different restriction sites we have introduced and the enzymes that target each site. Finally, the total length of our construction is indicated in the figure.
discontinued: false
dominant: false
experience: experience/Works
group u list: _673_
m user id: 0
owner id: 6159
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
star: false
creator: Victor Alvarez, Felix Reyes and Yolanda Gonzalez
type: DnaRegion
role: partType/Device
role: SO:0000804
sequence annotation: Double terminator
sequence annotation: Restriction Site: AflII
sequence annotation: Restriction Site: AscI
sequence annotation: GFP::Das4
sequence annotation: DAS4
sequence annotation: RBS
sequence annotation: Restriction Site: BamHI
sequence annotation: LacI::Das4
sequence annotation: PcI
sequence annotation: Restriction Site: Sma I
sequence annotation: PLac
sequence annotation: cI (Ts)
sequence annotation: Restriction Site: NheI
sequence annotation: RBS+OmpN::SspB
sequence annotation: RBS+OmpN
sequence annotation: Restriction Site: SacII
sequence annotation: RBS+OmpN::RFP
sequence annotation: Restriction Site: StuI
sequence: https://synbiohub.org/public/igem/BBa_K510019_sequence/1

Double terminator
type: SequenceAnnotation
persistent identity: annotation2136058
display id: annotation2136058
version: 1
top level: BBa_K510019
location: https://synbiohub.org/public/igem/BBa_K510019/annotation2136058/range2136058/1
role: SO:0000319
role: feature/stop

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type: Range
persistent identity: range2136058
display id: range2136058
version: 1
top level: BBa_K510019
direction: direction/reverse
start: 10
end: 138
orientation: inline

Restriction Site: AflII
type: SequenceAnnotation
persistent identity: annotation2136059
display id: annotation2136059
version: 1
top level: BBa_K510019
location: https://synbiohub.org/public/igem/BBa_K510019/annotation2136059/range2136059/1
role: feature/dna
role: SO:0000110

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type: Range
persistent identity: range2136059
display id: range2136059
version: 1
top level: BBa_K510019
direction: direction/forward
start: 139
end: 144
orientation: inline

Restriction Site: AscI
type: SequenceAnnotation
persistent identity: annotation2136060
display id: annotation2136060
version: 1
top level: BBa_K510019
location: https://synbiohub.org/public/igem/BBa_K510019/annotation2136060/range2136060/1
role: feature/dna
role: SO:0000110

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type: Range
persistent identity: range2136060
display id: range2136060
version: 1
top level: BBa_K510019
direction: direction/forward
start: 147
end: 154
orientation: inline

GFP::Das4
type: SequenceAnnotation
persistent identity: annotation2136061
display id: annotation2136061
version: 1
top level: BBa_K510019
location: https://synbiohub.org/public/igem/BBa_K510019/annotation2136061/range2136061/1
role: SO:0000316
role: feature/cds

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type: Range
persistent identity: range2136061
display id: range2136061
version: 1
top level: BBa_K510019
direction: direction/reverse
start: 155
end: 919
orientation: inline

DAS4
type: SequenceAnnotation
persistent identity: annotation2136062
display id: annotation2136062
version: 1
top level: BBa_K510019
location: https://synbiohub.org/public/igem/BBa_K510019/annotation2136062/range2136062/1
role: feature/tag
role: SO:0000324

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type: Range
persistent identity: range2136062
display id: range2136062
version: 1
top level: BBa_K510019
direction: direction/reverse
start: 161
end: 205
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2136063
display id: annotation2136063
version: 1
top level: BBa_K510019
location: https://synbiohub.org/public/igem/BBa_K510019/annotation2136063/range2136063/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K510019/annotation2136063/range2136063/1
type: Range
persistent identity: range2136063
display id: range2136063
version: 1
top level: BBa_K510019
direction: direction/reverse
start: 926
end: 938
orientation: inline

Restriction Site: BamHI
type: SequenceAnnotation
persistent identity: annotation2136064
display id: annotation2136064
version: 1
top level: BBa_K510019
location: https://synbiohub.org/public/igem/BBa_K510019/annotation2136064/range2136064/1
role: SO:0000110
role: feature/dna

https://synbiohub.org/public/igem/BBa_K510019/annotation2136064/range2136064/1
type: Range
persistent identity: range2136064
display id: range2136064
version: 1
top level: BBa_K510019
direction: direction/forward
start: 940
end: 945
orientation: inline

LacI::Das4
type: SequenceAnnotation
persistent identity: annotation2136065
display id: annotation2136065
version: 1
top level: BBa_K510019
location: https://synbiohub.org/public/igem/BBa_K510019/annotation2136065/range2136065/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K510019/annotation2136065/range2136065/1
type: Range
persistent identity: range2136065
display id: range2136065
version: 1
top level: BBa_K510019
direction: direction/reverse
start: 947
end: 2077
orientation: inline

DAS4
type: SequenceAnnotation
persistent identity: annotation2136066
display id: annotation2136066
version: 1
top level: BBa_K510019
location: https://synbiohub.org/public/igem/BBa_K510019/annotation2136066/range2136066/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_K510019/annotation2136066/range2136066/1
type: Range
persistent identity: range2136066
display id: range2136066
version: 1
top level: BBa_K510019
direction: direction/reverse
start: 953
end: 997
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2136067
display id: annotation2136067
version: 1
top level: BBa_K510019
location: https://synbiohub.org/public/igem/BBa_K510019/annotation2136067/range2136067/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K510019/annotation2136067/range2136067/1
type: Range
persistent identity: range2136067
display id: range2136067
version: 1
top level: BBa_K510019
direction: direction/reverse
start: 2084
end: 2096
orientation: inline

PcI
type: SequenceAnnotation
persistent identity: annotation2136068
display id: annotation2136068
version: 1
top level: BBa_K510019
location: https://synbiohub.org/public/igem/BBa_K510019/annotation2136068/range2136068/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K510019/annotation2136068/range2136068/1
type: Range
persistent identity: range2136068
display id: range2136068
version: 1
top level: BBa_K510019
direction: direction/reverse
start: 2105
end: 2153
orientation: inline

Restriction Site: Sma I
type: SequenceAnnotation
persistent identity: annotation2136069
display id: annotation2136069
version: 1
top level: BBa_K510019
location: https://synbiohub.org/public/igem/BBa_K510019/annotation2136069/range2136069/1
role: SO:0000110
role: feature/dna

https://synbiohub.org/public/igem/BBa_K510019/annotation2136069/range2136069/1
type: Range
persistent identity: range2136069
display id: range2136069
version: 1
top level: BBa_K510019
direction: direction/forward
start: 2171
end: 2176
orientation: inline

PLac
type: SequenceAnnotation
persistent identity: annotation2136070
display id: annotation2136070
version: 1
top level: BBa_K510019
location: https://synbiohub.org/public/igem/BBa_K510019/annotation2136070/range2136070/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K510019/annotation2136070/range2136070/1
type: Range
persistent identity: range2136070
display id: range2136070
version: 1
top level: BBa_K510019
direction: direction/forward
start: 2187
end: 2386
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation2136071
display id: annotation2136071
version: 1
top level: BBa_K510019
location: https://synbiohub.org/public/igem/BBa_K510019/annotation2136071/range2136071/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K510019/annotation2136071/range2136071/1
type: Range
persistent identity: range2136071
display id: range2136071
version: 1
top level: BBa_K510019
direction: direction/forward
start: 2395
end: 2407
orientation: inline

cI (Ts)
type: SequenceAnnotation
persistent identity: annotation2136072
display id: annotation2136072
version: 1
top level: BBa_K510019
location: https://synbiohub.org/public/igem/BBa_K510019/annotation2136072/range2136072/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K510019/annotation2136072/range2136072/1
type: Range
persistent identity: range2136072
display id: range2136072
version: 1
top level: BBa_K510019
direction: direction/forward
start: 2414
end: 3127
orientation: inline

Restriction Site: NheI
type: SequenceAnnotation
persistent identity: annotation2136073
display id: annotation2136073
version: 1
top level: BBa_K510019
location: https://synbiohub.org/public/igem/BBa_K510019/annotation2136073/range2136073/1
role: SO:0000110
role: feature/dna

https://synbiohub.org/public/igem/BBa_K510019/annotation2136073/range2136073/1
type: Range
persistent identity: range2136073
display id: range2136073
version: 1
top level: BBa_K510019
direction: direction/forward
start: 3130
end: 3135
orientation: inline

RBS+OmpN::SspB
type: SequenceAnnotation
persistent identity: annotation2136074
display id: annotation2136074
version: 1
top level: BBa_K510019
location: https://synbiohub.org/public/igem/BBa_K510019/annotation2136074/range2136074/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K510019/annotation2136074/range2136074/1
type: Range
persistent identity: range2136074
display id: range2136074
version: 1
top level: BBa_K510019
direction: direction/forward
start: 3139
end: 3732
orientation: inline

RBS+OmpN
type: SequenceAnnotation
persistent identity: annotation2136075
display id: annotation2136075
version: 1
top level: BBa_K510019
location: https://synbiohub.org/public/igem/BBa_K510019/annotation2136075/range2136075/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K510019/annotation2136075/range2136075/1
type: Range
persistent identity: range2136075
display id: range2136075
version: 1
top level: BBa_K510019
direction: direction/forward
start: 3139
end: 3234
orientation: inline

Restriction Site: SacII
type: SequenceAnnotation
persistent identity: annotation2136076
display id: annotation2136076
version: 1
top level: BBa_K510019
location: https://synbiohub.org/public/igem/BBa_K510019/annotation2136076/range2136076/1
role: feature/dna
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K510019/annotation2136076/range2136076/1
type: Range
persistent identity: range2136076
display id: range2136076
version: 1
top level: BBa_K510019
direction: direction/forward
start: 3735
end: 3740
orientation: inline

RBS+OmpN::RFP
type: SequenceAnnotation
persistent identity: annotation2136078
display id: annotation2136078
version: 1
top level: BBa_K510019
location: https://synbiohub.org/public/igem/BBa_K510019/annotation2136078/range2136078/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K510019/annotation2136078/range2136078/1
type: Range
persistent identity: range2136078
display id: range2136078
version: 1
top level: BBa_K510019
direction: direction/forward
start: 3742
end: 4518
orientation: inline

RBS+OmpN
type: SequenceAnnotation
persistent identity: annotation2136077
display id: annotation2136077
version: 1
top level: BBa_K510019
location: https://synbiohub.org/public/igem/BBa_K510019/annotation2136077/range2136077/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K510019/annotation2136077/range2136077/1
type: Range
persistent identity: range2136077
display id: range2136077
version: 1
top level: BBa_K510019
direction: direction/forward
start: 3742
end: 3837
orientation: inline

Restriction Site: StuI
type: SequenceAnnotation
persistent identity: annotation2136079
display id: annotation2136079
version: 1
top level: BBa_K510019
location: https://synbiohub.org/public/igem/BBa_K510019/annotation2136079/range2136079/1
role: feature/dna
role: SO:0000110

https://synbiohub.org/public/igem/BBa_K510019/annotation2136079/range2136079/1
type: Range
persistent identity: range2136079
display id: range2136079
version: 1
top level: BBa_K510019
direction: direction/forward
start: 4526
end: 4531
orientation: inline

Double terminator
type: SequenceAnnotation
persistent identity: annotation2136080
display id: annotation2136080
version: 1
top level: BBa_K510019
location: https://synbiohub.org/public/igem/BBa_K510019/annotation2136080/range2136080/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K510019/annotation2136080/range2136080/1
type: Range
persistent identity: range2136080
display id: range2136080
version: 1
top level: BBa_K510019
direction: direction/forward
start: 4534
end: 4662
orientation: inline

https://synbiohub.org/public/igem/BBa_K510046_sequence/1
type: Sequence
persistent identity: BBa K510046 sequence
display id: BBa_K510046_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactagtagcggccgctgcagtccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaataggccaaccagataagtgaaatctagttccaaactattttgtcatttttaattttcgtattagcttacgacgctacacccagttcccatctattttgtcactcttccctaaataatccttaaaaactccatttccacccctcccagttcccaactattttgtccgcccacaggccgcctaggccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgttaaggtatactttccgctgcataaccctgcttcggggtcattatagcgattttttcggtatatccatcctttttcgcacgatatacaggattttgccaaagggttcgtgtagactttccttggtgtatccaacggcgtcagccgggcaggataggtgaagtaggcccacccgcgagcgggtgttccttcttcactgtcccttattcgcacctggcggtgctcaacgggaatcctgctctgcgaggctggccgataagctctgataacgcaggaaagaacatgggcagttcaacctgttgatagtacgtactaagctctcatgtttcacgtactaagctctcaagtttaacgtactaagctctcatgtttaacgaactaaaccctcatggctaacgtactaagctctcatggctaacgtactaagctctcatgtttcacgtactaagctctcatgtttgaacaataaaattaatataaatcagcaacttaaatagcctctaaggttttaagttttataagaaaaaaaagaatatataaggcttttaaagcttttaaggtttaacggttgtggacaacaagccagggatgtaacgcactgagaagcccttagagcctctcaaagcaattttgagtgacacaggaacacttaacggctgacaaccggctggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagtta…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K510012_sequence/1
type: Sequence
persistent identity: BBa K510012 sequence
display id: BBa_K510012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tactagtagcggccgctgcagtccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaataggccaaccagataagtgaaatctagttccaaactattttgtcatttttaattttcgtattagcttacgacgctacacccagttcccatctattttgtcactcttccctaaataatccttaaaaactccatttccacccctcccagttcccaactattttgtccgcccacaggccgcctaggccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgttaaggtatactttccgctgcataaccctgcttcggggtcattatagcgattttttcggtatatccatcctttttcgcacgatatacaggattttgccaaagggttcgtgtagactttccttggtgtatccaacggcgtcagccgggcaggataggtgaagtaggcccacccgcgagcgggtgttccttcttcactgtcccttattcgcacctggcggtgctcaacgggaatcctgctctgcgaggctggccgataagctctgataacgcaggaaagaacatgggcagttcaacctgttgatagtacgtactaagctctcatgtttcacgtactaagctctcaagtttaacgtactaagctctcatgtttaacgaactaaaccctcatggctaacgtactaagctctcatggctaacgtactaagctctcatgtttcacgtactaagctctcatgtttgaacaataaaattaatataaatcagcaacttaaatagcctctaaggttttaagttttataagaaaaaaaagaatatataaggcttttaaagcttttaaggtttaacggttgtggacaacaagccagggatgtaacgcactgagaagcccttagagcctctcaaagcaattttgagtgacacaggaacacttaacggctgacaaccggctggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagtta…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K510019_sequence/1
type: Sequence
persistent identity: BBa K510019 sequence
display id: BBa_K510019_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gagatcatttataaacgcagaaaggcccacccgaaggtgagccagtgtgactctagtagagagcgttcaccgacaaacaacagataaaacgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggcttaagtaggcgcgccctattaagaagcatcagcataattttcagagtagttctcgtcgtttgctgctttgtatagttcatccatgccatgtgtaatcccagcagctgttacaaactcaagaaggaccatgtggtctctcttttcgttgggatctttcgaaagggcagattgtgtggacaggtaatggttgtctggtaaaaggacagggccatcgccaattggagtattttgttgataatggtctgctagttgaacgcttccatcttcaatgttgtgtctaattttgaagttaactttgattccattcttttgtttgtctgccatgatgtatacattgtgtgagttatagttgtattccaatttgtgtccaagaatgtttccatcttctttaaaatcaataccttttaactcgattctattaacaagggtatcaccttcaaacttgacttcagcacgtgtcttgtagttcccgtcatctttgaaaaatatagttctttcctgtacataaccttcaggcatggcactcttgaaaaagtcatgctgtttcatatgatctgggtatctcgcaaagcattgaacaccataaccgaaagtagtgacaagtgttggccatggaacaggtagttttccagtagtgcaaataaatttaagggtaagttttccgtatgttgcatcaccttcaccctctccactgacagaaaatttgtgcccattaacatcaccatctaattcaacaagaattgggacaactccagtgaaaagttcttctcctttactcatctagtactttcctgtgtgacggatccactatcaagaagcatcagcataattttcagagtagttctcgtcgtttgctgcctgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgccagggtggtttttcttttcaccagtgagacgggcaacagctgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgccccagcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtcttcggtatcgtcgtatcccactaccgagatatccgcaccaacgcgcagcccggactcggtaatggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacgatgccctcattcagcatttgcatggtttgttgaaaaccggacatggcactccagtcgccttcccgttccgctatcggctgaatttgattgcgagtgagatatttatgccagccagccagacgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgacccaatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactgttgatgggtgtctggtcagagacatcaagaaataacgccggaacattagtgcaggcagcttccacagcaatggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgttgcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctaccatcgacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgcgacggcgcgtgcagggccagactggaggtggcaacgccaatcagcaacgactgtttgcccgccagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgcttccacttcttcccgcgttttcgcagaaacgtggctggcc…
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z