BBa_K511003
1
BBa_K511003
UAS-Gal4 Promoter MammoBlock
2011-09-24T11:00:00Z
2015-05-08T01:12:31Z
This part was synthesized to include the upstream activator sequence (UAS) from the Saccharomyces cerevisiae genome that binds to the Gal4 transactivator upstream of the major-late adenovirus promoter.
This part encodes a promoter that is inducible by variants of the Gal4 transactivator and off otherwise.
As shown in Figures 1 and 2, fluorescence from a red fluorescent protein (mKate) driven by the UAS-Gal4 promoter is very strong in the presence of constitutively produced Gal4 derivative with an attached viral VP16 transactivation domain (Gal4-VP16), and is essentially off in the absences of the Gal4-VP16 transactivator.
false
false
_674_
0
6719
9
It's complicated
true
Not applicable.
false
Grant Robinson
annotation2139553
1
Major-Late Adenovirus Promoter
range2139553
1
160
200
annotation2139552
1
Gal4-Binding Region
range2139552
1
46
148
BBa_K511003_sequence
1
gctccgaattgggacagcagagatccagtttggttaattaatagacggagtactgtcctccgagcggagtactgtcctccgactcgagcggagtactgtcctccgatcggagtactgtcctccgcgaattccggagtactgtcctccgaagacgctagcggggggctataaaagggggtgggggcgttcgtcctcactctcaattcggcccaattcgacc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z