BBa_K511101 1 BBa_K511101 EYFP-FF4x4 MammoBlock 2011-09-24T11:00:00Z 2015-05-08T01:12:31Z This protein is an engineered version of the original green fluorescent protein from Aequorea victoria that has ben modified to fluoresce yellow. This part is an engineered variant of the original green fluorescent protein from Aequorea victoria that fluoresces yellow. Additionally, this protein has four binding sites for the FF4 micro RNA (miRNA). In the presence of FF4 miRNA, translation of this protein is markedly attenuated. Figures 1 and 2 show typical levels of EYFP-FF4x4-mediated fluorescence following transfection of HEK-293 cells with EYFP-FF4x4 driven by the Hef1a-LacOid low-level constitutive promoter. false false _674_ 0 6719 9 It's complicated false The presence of FF4 binding sites on this protein will preclude its use in certain cells that naturally produce the FF4 miRNA, and it is recommended that parties interested in using this part consult references explaining which miRNAs are produced in their desired cell types. false Grant Robinson annotation2139559 1 Four FF4 Binding Sites range2139559 1 732 819 annotation2139558 1 Enhanced Yellow Fluorescent Protein range2139558 1 1 720 BBa_K511101_sequence 1 atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcagtgcttcgcccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccaagctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaaagcggcgcgccccgcttgaagtctttaattaaaccgcttgaagtctttaattaaaccgcttgaagtctttaattaaaccgcttgaagtctttaattaaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z