BBa_K511202 1 BBa_K511202 rtTA-DD Transactivator MammoBlock 2011-09-24T11:00:00Z 2015-05-08T01:12:31Z This part consists of an Escherichia coli-derived binding domain for the tetracycline operator, the viral VP16 transactivation domain isolated from the herpes simplex virus, and an engineered human FKBP12 protein that functions as a destabilization domain. This part encodes a protein that combines the rtTA transactivator with the DD destabilization domain. The DD domain causes attached proteins to be degraded extremely quickly in mammalian cells; however, the DD domain is stable in the presence of the SHIELD molecule, allowing for tunable protein expression. Because the rtTA transactivator is dependent upon doxycycline for activation, transactivation by rtTA-DD is unique among transactivators for the dynamic range of expression it can activate. false false _674_ 0 6719 9 It's complicated false Note that the DD domain is positioned before the rtTA transactivator in this part. false Grant Robinson annotation2139589 1 rtTA Transactivation Domain range2139589 1 325 1329 annotation2139588 1 Destabilization Domain range2139588 1 1 324 BBa_K511202_sequence 1 atgggcgtgcaggtggagaccatcagcccaggagatggcaggacattccccaagcgcgggcagacttgcgtggtgcactacaccggcatgctggaggacgggaagaaagtggatagctccagggaccgcaacaagcccttcaagttcatgctgggaaagcaggaagtgatccgcgggtgggaggaaggagtggcccagatgagtgtgggccagcgggctaaactgactatttcacctgactacgcctatggagctaccggccacccagggatcattccccctcatgccaccctggtgttcgatgtggaactgctgaagccagagatgtcaagactggacaagagcaaagtgatcaactccgccctggagctgctgaatgaagtgggaattgagggcctgaccacaaggaagctggctcagaaactgggcgtggagcagccaactctgtattggcacgtgaagaacaaacgcgccctgctggatgccctggctattgagatgctggaccggcaccatacacatttttgccccctgaagggcgaaagctggcaggatttcctgcggaacaatgccaaatcttttagatgtgctctgctgagtcacaggaatggggccaaggtgcatagcgacacacgccccactgagaaacagtacgagaccctggaaaaccagctggccttcctgtgccagcagggcttttccctggaaaatgccctgtatgctctgtctgccgtgggacacttcacactgggctgcgtgctggaggatcaggaacatcaggtggccaaggaggaaagagagactcccactaccgattccatgccacccctgctgaggcaggccatcgaactgtttgaccaccagggcgctgagcctgccttcctgtttggcctggaactgatcatttgcgggctggaaaagcagctgaaatgcgagtctggcagtgcttacagccgggccagaacaaaaaacaattatgggtccactattgagggactgctggacctgccagacgatgacgctccagaggaagctggactggctgctccaaggctgtctttcctgcctgccggacacacccggagactgtcaaccgctcctccaacagatgtgagcctgggagacgaactgcacctggatggcgaggacgtggccatggctcatgccgatgctctggatgacttcgatctggacatgctgggggatggagactctccaggacctgggtttacccctcatgacagtgctccatacggggccctggatatggctgacttcgagtttgaacagatgtttacagatgccctgggaatcgacgagtatggcggctgatga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z