BBa_K511303 1 BBa_K511303 Delta-mCherry-4xFF4 Juxtacrine Signaling Ligand MammoBlock 2011-09-26T11:00:00Z 2015-05-08T01:12:31Z This part is composed of the Delta-3 ligand from Rattus norvegicus and the synthetic mCherry fluorescent protein engineered from the green fluorescent protein isolated from Aequorea victoria; the sequence for the four FF4 micro RNA (miRNA) tags was synthetically produced. This part encodes a fusion between the Delta-3 ligand and the monomeric mCherry red fluorescent protein. Additionally, the protein is succeeded by four copies of the FF4 intronic miRNA binding sequence, allowing for miRNA-mediated repression of this part's translation. Figures 1 and 2 below show fluorescent output from the Delta-mCherry protein, while Figures 3 and 4 below show transactivation of the Notch-Gal4-ESN receptor by the Delta-mCherry protein. false false _674_ 0 6719 9 It's complicated false Due to the high secondary structure of FF4 miRNA, this part was assembled with standard restriction cloning methods, specifically using enzymes AscI and BamHI. false Grant Robinson annotation2144635 1 Four FF4 Binding Sites range2144635 1 2862 2949 annotation2144632 1 Delta-3 Juxtacrine Signaling Ligand range2144632 1 1 2142 annotation2144633 1 mCherry Fluorescent Protein range2144633 1 2143 2850 annotation2144634 1 Stop Codon range2144634 1 2851 2853 BBa_K511303_sequence 1 atgggccgtcggagcgctctagcccttgccgtggtctcagccctgctgtgccaggtctggagctctggcgtatttgagctgaagctgcaggagttcgtcaacaagaaggggctgctggggaaccgcaactgctgccgcgggggctctggcccgccgtgcgcctgcaggaccttctttcgcgtatgcctcaagcattaccaggccagcgtgtccccggagccaccctgcacctacggcagtgcggtcaccgcagtgctgggtgtcgactccttcagcctgcctgatggcgcaggcatcgaccccgccttcagcaaccccatccgattccccttcggattcacctggccaggtaccttctctctgatcattgaagccctccacacagattctcctgacgacctcgcaacagaaaacccagaaagactcatcagccgcctgaccacacagaggcacctcactgtgggagaagagtggtctcaggaccttcacagtagcggccgcacagacctccgctactcttaccggtttgtgtgtgatgaacactactatggagaaggctgctccgtgttctgccgaccgcgggatgatgcctttggccacttcacctgcggggagagaggggagaagatgtgcgaccctggctggaaaggccagtactgcactgaccccatttgtctgccaggctgtgatgaccaacatggatattgtgacaaaccgggggaatgcaagtgcagagttggctggcagggccgctactgcgatgaatgcatccgatacccaggctgtctccatggtacctgccagcagccctggcagtgtaactgccaggaaggctgggggggcctcttctgcaaccaggatctgaactactgcactcaccataagccatgcaggaacggagccacctgcaccaacacgggccaggggagctacacatgctcttgccgacccgggtatacaggggccaactgtgagctggaggtagatgagtgtgctcccagcccctgcaggaatggagggagctgcacggatcttgaggacagctactcttgcacctgccctcctggcttctatggcaaggtctgtgagctgagcgccatgacgtgtgcagatggtccttgcttcaatgggggacgatgttcggataaccccgatggaggctacacctgccattgccctgcgggcttctctggcttcaactgtgagaagaagattgatctctgtagctcttccccttgttctaacggtgccaagtgtgtggacctcggcaactcctacctgtgccgatgtcagactggcttctccgggaggtactgcgaggacaatgtggatgactgtgcctcttctccctgtgcaaacgggggcacctgccgggacagtgtgaacgatttctcctgtacctgcccacctggctacacaggcaggaactgcagcgcccctgtcagcaggtgtgagcatgcaccctgtcataacggggccacctgccaccagaggggccaacgctacatgtgtgagtgcgcccagggctatggcggcgccaactgccagttcctgctccctgagccaccaccagacctcatagtggcggcccagggcgggtccttcccctgggtggctgtgtgtgccggggtggtgcttgtcctcctgctgctgctgggctgtgctgctgtggtggtctgcgtccggctgaagctacagaaacaccagcctccgcctgatccttgcgggggagagacagagaccatgaacaacctagccaattgccagcgtgagaaggatgtttctgttagcatcattggggctacacagatcaagaacaccaacaagaaggcggactttcatggggaccatggtgctgacaagagcagctttaaggcccgataccccactgtggactataacctcattcgagacctcaagggagatgaagccacggtcagggatgcacacagcaaacgtgacaccaagtgccagtcacagggctctgtaggagaagagaagagcacctcaacgctcaggggtggggaggttcccgacagaaaaaggcccgagtctgtctactctacttcaaaggacaccaagtaccagtcggtgtatgttctatctgcagaaaaggatgagtgtgttatagcgactgaggttgtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccctgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtcttagggcgcgccccgcttgaagtctttaattaaaccgcttgaagtctttaattaaaccgcttgaagtctttaattaaaccgcttgaagtctttaattaaagctagt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z