BBa_K511401 1 BBa_K511401 HRH4-TEVs-GV16 Signaling Receptor MammoBlock 2011-09-26T11:00:00Z 2015-05-08T01:12:31Z This part includes human histamine receptor 4 (HRH4), the tobacco etch virus (TEV) protease recognition sequence, the DNA-binding domain from the Gal4 transactivator of Saccharomyces cerevisiae, and the VP16 transactivation domain from the herpes simplex virus. This part encodes a fusion protein consisting of the human HRH4 receptor, the recognition sequence for the tobacco etch virus (TEV) protease, and the Gal4-VP16 transactivator. false false _674_ 0 6719 9 It's complicated false The TEV recognition sequence encoded in the rough center of this part is flanked on both sides by sequences that code for glycine-serine-glycine linkers. This feature was designed to serve as a homologous region allowing for facile substitution of either a receptor or a transactivator around the TEV recognition sequence using the Gibson ligation assembly method. false Grant Robinson annotation2144659 1 HRH4 Receptor range2144659 1 1 1177 annotation2144660 1 Flexible TEV Recognition Sequence range2144660 1 1177 1216 annotation2144661 1 Gal4-VP16 Transactivator range2144661 1 1217 1896 BBa_K511401_sequence 1 agccaccatgccagatactaatagcacaatcaatttatcactaagcactcgtgttactttagcattttttatgtccttagtagcttttgctataatgctaggaaatgctttggtcattttagcttttgtggtggacaaaaaccttagacatcgaagtagttatttttttcttaacttggccatctctgacttctttgtgggtgtgatctccattcctttgtacatccctcacacgctgttcgaatgggattttggaaaggaaatctgtgtattttggctcactactgactatctgttatgtacagcatctgtatataacattgtcctcatcagctatgatcgatacctgtcagtctcaaatgctgtgtcttatagaactcaacatactggggtcttgaagattgttactctgatggtggccgtttgggtgctggccttcttagtgaatgggccaatgattctagtttcagagtcttggaaggatgaaggtagtgaatgtgaacctggatttttttcggaatggtacatccttgccatcacatcattcttggaattcgtgatcccagtcatcttagtcgcttatttcaacatgaatatttattggagcctgtggaagcgtgatcatctcagtaggtgccaaagccatcctggactgactgctgtctcttccaacatctgtggacactcattcagaggtagactatcttcaaggagatctctttctgcatcgacagaagttcctgcatcctttcattcagagagacagaggagaaagagtagtctcatgttttcctcaagaaccaagatgaatagcaatacaattgcttccaaaatgggttccttctcccaatcagattctgtagctcttcaccaaagggaacatgttgaactgcttagagccaggagattagccaagtcactggccattctcttaggggtttttgctgtttgctgggctccatattctctgttcacaattgtcctttcattttattcctcagcaacaggtcctaaatcagtttggtatagaattgcattttggcttcagtggttcaattcctttgtcaatcctcttttgtatccattgtgtcacaagcgctttcaaaaggctttcttgaaaatattttgtataaaaaagcaacctctaccatcacaacacagtcggtcagtatcttctggatccggagagaacctgtacttccagctcggatccggagcccccccgaccgatgtcagcctgggggacgagctccacttagacggcgaggacgtggcgatggcgcatgccgacgcgctagacgatttcgatctggacatgttgggggacggggattccccgggtccgggatttaccccccacgactccgccccctacggcgctctggatatggccgacttcgagtttgagcagatgtttaccgatgcccttggaattgacgagtacggtgggacgcgtatgaagctactgtcttctatcgaacaagcatgcgatatttgccgacttaaaaagctcaagtgctccaaagaaaaaccgaagtgcgccaagtgtctgaagaacaactgggagtgtcgctactctcccaaaaccaaaaggtctccgctgactagggcacatctgacagaagtggaatcaaggctagaaagactggaacagctatttctactgatttttcctcgagaagaccttgacatgattttgaaaatggattctttacaggatataaaagcattgttaacaggattatttgtacaagataatgtgaataaagatgccgtcacagatagattggcttcagtggagactgatatgcctctaacattgagacagcatagaataagtgcgacatcatcatcggaagagagtagtaacaaaggtcaaagacagttgactgtataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z