BBa_K511600 1 BBa_K511600 N-Cadherin-2A-EYFP Cell Adhesion MammoBlock 2011-09-26T11:00:00Z 2015-05-08T01:12:31Z The N-Cadherin sequence of this part was isolated from a murine source; the viral 2A self-cleaving peptide was isolated from a virus with homology to the foot-and-mouth disease virus; the EYFP fluorescent reporter is an engineered version of the original green fluorescent protein from Aequorea victoria. This part encodes a protein kmown to be instrumental in intercellular adhesion and tissue integrity. Like all cadherins, N-Cadherin mediates adhesion with five extracellular, calcium-binding repeats and an intercellular domain that makes contacts with the actin cytoskeleton of mammalian cells. This part additionally encodes an enhanced yellow fluorescent protein (EYFP) linked to the N-Cadherin by a self-cleaving viral 2A peptide, allowing for bicistronic expression of both a cell adhesion protein and an identifying marker with a single part. false false _674_ 0 6719 9 It's complicated false Not applicable. false Grant Robinson annotation2144667 1 Murine N-Cadherin range2144667 1 1 2718 annotation2144668 1 2A Self-Cleaving Peptide range2144668 1 2719 2794 annotation2144670 1 EYFP range2144670 1 2719 3511 BBa_K511600_sequence 1 atgtgccggatagcgggagcgccgcggaccctgctgccgcttctggcggccttgcttcaggcgtctgtggaggcttctggtgaaattgcattatgcaagactggatttcctgaagatgtttacagcgcagtcttaccgaaggatgtgcacgaaggacagccccttctcaatgtgaaattcagcaactgcaatagaaaaaggaaagttcagtatgaaagcagcgagccagcagatttcaaggtggacgaggacggcacggtgtatgctgtgagaagcttccctctcactgcagagcaggcaaagttcctgatatatgcccaagacaaagaaacccaggaaaagtggcaggtagctgtaaacctgagccgggagccaaccctgactgaggagcctatgaaggaaccacatgaaattgaagaaatagtattccctagacaacttgccaagcacagtggagctctacaaaggcagaagagagactgggtcatcccgccaatcaacttgccagaaaactccagaggaccctttcctcaagagcttgtcagaatcaggtctgatagagataaaaacctttccctgagatacagcgtcactgggccaggagctgaccagcctccaacgggcatcttcattatcaaccccatctcaggacagctgtcagtcacaaagcctctggatcgagagctgatagcccggtttcacttgagagcacatgcagtggacatcaatggcaatcaagtggagaaccccattgacattgtcatcaatgttattgacatgaatgataacagacctgagtttctgcaccaggtttggaatgggtctgttccagagggatcaaagcctgggacgtatgtgatgacggtcactgccattgatgcggatgatccaaatgccctgaatggaatgctgcggtacaggatcctgtcccaggcgcccagcacaccttcacccaacatgtttacaatcaacaatgagactggggacatcatcactgtggcagctggtctggatcgagagaaagtgcaacagtatacgttaataattcaagccacagacatggaaggcaatcccacttatggcctttcaaacacagccacagccgtcatcacggtgacagatgtcaatgacaatcctccagagtttactgccatgactttctacggagaagtccctgagaacagggtggacgtcattgtagccaacctaactgtcacggacaaagatcagccccacacgccggcctggaatgcggcatacagaatcagtggtggagaccctacaggaaggtttgccatcctgacagaccccaacagcaatgatgggctagtcacagtggtaaaaccaattgactttgaaacgaataggatgtttgtccttactgttgctgcagaaaaccaagtgccattagctaaaggcattcagcacccacctcagtcgacagccactgtgtctgtgacagttattgatgtcaatgaaaatccttattttgccccaaatcctaaaatcattcgccaagaggaaggcctccacgcaggtaccatgctgaccacgctcactgctcaggaccccgatcgatatatgcaacagaatatcagatacacaaaattgtctgatcctgccaactggctgaaaatagaccccgtgaatgggcagatcactactattgccgttttggacagagaatcgccaaatgtaaaaaacaacatctataatgctaccttccttgcttctgacaatggaatcccgcctatgagtgggacaggaacactgcaaatctatttacttgatatcaatgacaacgcccctcaggtgttacctcaagaggcagagacctgtgaaactccagaacccaactcaattaacatcacagcacttgattatgacatagacccaaacgccgggccattcgcgtttgatcttcccttatctccagtgactattaaaagaaactggaccatcaaccggcttaatggtgattttgctcagctcaatttaaagataaaatttttggaagctggtatctatgaagttcccatcattatcacagattcagggaatccccccaagtccaacatttccatcctgcgtgtgaaagtttgccagtgtgactccaatggagactgcacggacgtggacaggatcgtgggtgcagggcttggcacgggtgccatcatcgctatccttctgtgtatcatcatcctgctgatccttgttctcatgtttgtggtatggatgaaacggcgggataaagagcgccaagccaagcagcttttaattgacccagaagatgatgtaagagataatatattgaaatatgatgaagaaggtggaggagaagaagaccaggactatgacttgagccagctccagcaaccagatactgtggagcctgatgccatcaagcccgtgggaatcagacggctagacgagaggcctatccatgctgagccacagtacccagtccgatccgcagccccacaccctggggatattggggacttcattaatgagggccttaaagctgctgacaacgaccccacggcgccaccgtatgactccctcttagtctttgactacgagggcagcggctccacggctggctccttgagctccctcaactcctccagtagcggtggggaccaggactatgactacctgaatgactggggaccccgcttcaagaaactggcggacatgtacggcggtggtgacgacagtcactagtggatctggcgccaccaacttctctctgctgaagcaggccggcgacgtggaggagaacccaggcccaatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccttcggctacggcctgcagtgcttcgcccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcggcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagctaccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z