BBa_K511701 1 BBa_K511701 B-Arrestin-2-TEV-Protease MammoBlock 2011-09-26T11:00:00Z 2015-05-08T01:12:31Z The N1a domain was isolated from the tobacco etch mosaic virus while the B-Arrestin-2 protein was isolated from a human cDNA library. This part encodes a fusion between the N1a catalytic proteolytic domain of the tobacco etch virus (TEV) and the human B-Arrestin-2 sequence. The N1a domain recognizes the amino acid sequence ENLYFQX (where X is a variable amino acid) with unparalleled specificity, and is typically used for the removal of tags made for protein purification. The B-Arrestin-2 protein induces the extinction of nonvisual G-coupled protein receptor (GPCR) signaling in mammalian cells by binding to the cytoplasmic tails of induced GPCRs. This part is capable of cleaving GPCR-TEVs-Transactivator fusion proteins and freeing the tethered transactivator to induce transcriptional changes in a cell's nucleus. false false _674_ 0 6719 9 It's complicated false Not applicable. false Grant Robinson annotation2144704 1 B-Arrestin-2 range2144704 1 1 1233 annotation2144705 1 Double-Glycine Linker range2144705 1 1234 1239 annotation2144706 1 TEV Protease range2144706 1 1240 1890 BBa_K511701_sequence 1 gccaccatgggggagaaacccgggaccagggtcttcaagaagtcgagccctaactgcaagctcaccgtgtacttgggcaagcgggacttcgtagatcacctggacaaagtggaccctgtagatggcgtggtgcttgtggaccctgactacctgaaggaccgcaaagtgtttgtgaccctcacctgcgccttccgctatggccgtgaagacctggatgtgctgggcttgtccttccgcaaagacctgttcatcgccacctaccaggccttccccccggtgcccaacccaccccggccccccacccgcctgcaggaccggctgctgaggaagctgggccagcatgcccaccccttcttcttcaccataccccagaatcttccatgctccgtcacactgcagccaggcccagaggatacaggaaaggcctgcggcgtagactttgagattcgagccttctgtgctaaatcactagaagagaaaagccacaaaaggaactctgtgcggctggtgatccgaaaggtgcagttcgccccggagaaacccggcccccagccttcagccgaaaccacacgccacttcctcatgtctgaccggtccctgcacctcgaggcttccctggacaaggagctgtactaccatggggagcccctcaatgtaaatgtccacgtcaccaacaactccaccaagaccgtcaagaagatcaaagtctctgtgagacagtacgccgacatctgcctcttcagcaccgcccagtacaagtgtcctgtggctcaactcgaacaagatgaccaggtatctcccagctccacattctgtaaggtgtacaccataaccccactgctcagcgacaaccgggagaagcggggtctcgccctggatgggaaactcaagcacgaggacaccaacctggcttccagcaccatcgtgaaggagggtgccaacaaggaggtgctgggaatcctggtgtcctacagggtcaaggtgaagctggtggtgtctcgaggcggggatgtctctgtggagctgccttttgttcttatgcaccccaagccccacgaccacatccccctccccagaccccagtcagccgctccggagacagatgtccctgtggacaccaacctcattgaatttgataccaactatgccacagatgatgacattgtgtttgaggactttgcccggcttcggctgaaggggatgaaggatgacgactatgatgatcaactctgcggaggaggaagcctgttcaagggccccagagactacaaccctatcagcagcaccatctgccacctgaccaacgagagcgacggccacaccaccagcctgtacggcatcggcttcggccccttcatcatcaccaacaagcacctgttccggcgaaacaacggcaccctgctggtgcagagcctgcacggcgtgttcaaagtgaagaataccacaaccctgcagcagcacctgatcgacggccgggacatgatcatcatccggatgcccaaggacttcccacccttcccacagaagctgaagttcagagagccccagcgcgaggaacggatctgtctggtcacaacaaacttccagaccaagagcatgagcagcatggtgtccgataccagctgcaccttccccagcagcgacggcatcttctggaagcactggattcagaccaaggacggccagtgcggcagtccactggtgtccacccgggatggcttcatcgtgggcatccacagcgccagcaacttcaccaacaccaacaactacttcaccagcgtgcccaagaacttcatggaactgctgaccaaccaggaagcccagcagtgggtctccggctggcggctgaacgccgattctgtgctgtggggcggacacaaggtgttcatgtga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z