BBa_K415508 1 BBa_K415508 phEF1a L4R1 MammoBlock Entry Vector 2010-10-26T11:00:00Z 2015-05-08T01:12:27Z MIT iGEM 2010 Mammalian Part MIT iGEM 2010 Mammalian Part false false _525_ 0 7331 9 It's complicated false MIT iGEM 2010 Mammalian Part false Laura Deming, Joy Jiao, Adrian Slusarczyk BBa_J85600 1 BBa_J85600 attB1 recombination site 2011-09-05T11:00:00Z 2015-05-08T01:08:30Z lambda phage attB1 recombination site Used for creating mammoblock (RFC65) composite promoter-gene pairs using recombination-based cloning. false false _406_ 0 106 406 Not in stock false None false Ron Weiss annotation2125966 1 attB1 range2125966 1 2 22 BBa_K511302 1 BBa_K511302 Delta-mCherry Juxtacrine Signaling Ligand MammoBlock 2011-09-26T11:00:00Z 2015-05-08T01:12:31Z This part is composed of the Delta-3 ligand from Rattus norvegicus and the synthetic mCherry fluorescent protein engineered from the green fluorescent protein isolated from Aequorea victoria. Additionally, the sequence for this part was included in a plasmid provided to the MIT iGEM 2011 team courtesy of the Elowitz lab at Caltech. This part encodes a fusion between the Delta-3 ligand and the monomeric mCherry red fluorescent protein. false false _674_ 0 6719 9 It's complicated true In the original plasmid provided, the Delta-mCherry sequence did not have a stop codon, resulting in plasmid read-through of about nine additional amino acids; our Delta-mCherry includes a stop codon immediately after the mCherry sequence. Both plasmids are phenotypically correct. false Grant Robinson annotation2144603 1 Delta-3 Juxtacrine Signaling Ligand range2144603 1 1 2142 annotation2144623 1 Stop Codon range2144623 1 2851 2853 annotation2144604 1 mCherry Fluorescent Protein range2144604 1 2143 2850 BBa_K511802 1 BBa_K511802 Red Fluorescent Delta Ligand Generator (Hef1a-Delta-mCherry) MammoBlock Device 2011-09-27T11:00:00Z 2015-05-08T01:12:31Z See component parts. This MammoBlock composite device produces the red fluorescent juxtacrine signaling ligand Delta-mCherry at a low, constitutive level. false false _674_ 0 6719 9 Not in stock false Not applicable. false Grant Robinson component2145974 1 BBa_J85600 component2145978 1 BBa_K511302 component2145972 1 BBa_K415508 annotation2145978 1 BBa_K511302 range2145978 1 1266 4118 annotation2145972 1 BBa_K415508 range2145972 1 1 1243 annotation2145974 1 BBa_J85600 range2145974 1 1244 1265 BBa_J85600_sequence 1 ccaagtttgtacaaaaaagcag BBa_K511302_sequence 1 atgggccgtcggagcgctctagcccttgccgtggtctcagccctgctgtgccaggtctggagctctggcgtatttgagctgaagctgcaggagttcgtcaacaagaaggggctgctggggaaccgcaactgctgccgcgggggctctggcccgccgtgcgcctgcaggaccttctttcgcgtatgcctcaagcattaccaggccagcgtgtccccggagccaccctgcacctacggcagtgcggtcaccgcagtgctgggtgtcgactccttcagcctgcctgatggcgcaggcatcgaccccgccttcagcaaccccatccgattccccttcggattcacctggccaggtaccttctctctgatcattgaagccctccacacagattctcctgacgacctcgcaacagaaaacccagaaagactcatcagccgcctgaccacacagaggcacctcactgtgggagaagagtggtctcaggaccttcacagtagcggccgcacagacctccgctactcttaccggtttgtgtgtgatgaacactactatggagaaggctgctccgtgttctgccgaccgcgggatgatgcctttggccacttcacctgcggggagagaggggagaagatgtgcgaccctggctggaaaggccagtactgcactgaccccatttgtctgccaggctgtgatgaccaacatggatattgtgacaaaccgggggaatgcaagtgcagagttggctggcagggccgctactgcgatgaatgcatccgatacccaggctgtctccatggtacctgccagcagccctggcagtgtaactgccaggaaggctgggggggcctcttctgcaaccaggatctgaactactgcactcaccataagccatgcaggaacggagccacctgcaccaacacgggccaggggagctacacatgctcttgccgacccgggtatacaggggccaactgtgagctggaggtagatgagtgtgctcccagcccctgcaggaatggagggagctgcacggatcttgaggacagctactcttgcacctgccctcctggcttctatggcaaggtctgtgagctgagcgccatgacgtgtgcagatggtccttgcttcaatgggggacgatgttcggataaccccgatggaggctacacctgccattgccctgcgggcttctctggcttcaactgtgagaagaagattgatctctgtagctcttccccttgttctaacggtgccaagtgtgtggacctcggcaactcctacctgtgccgatgtcagactggcttctccgggaggtactgcgaggacaatgtggatgactgtgcctcttctccctgtgcaaacgggggcacctgccgggacagtgtgaacgatttctcctgtacctgcccacctggctacacaggcaggaactgcagcgcccctgtcagcaggtgtgagcatgcaccctgtcataacggggccacctgccaccagaggggccaacgctacatgtgtgagtgcgcccagggctatggcggcgccaactgccagttcctgctccctgagccaccaccagacctcatagtggcggcccagggcgggtccttcccctgggtggctgtgtgtgccggggtggtgcttgtcctcctgctgctgctgggctgtgctgctgtggtggtctgcgtccggctgaagctacagaaacaccagcctccgcctgatccttgcgggggagagacagagaccatgaacaacctagccaattgccagcgtgagaaggatgtttctgttagcatcattggggctacacagatcaagaacaccaacaagaaggcggactttcatggggaccatggtgctgacaagagcagctttaaggcccgataccccactgtggactataacctcattcgagacctcaagggagatgaagccacggtcagggatgcacacagcaaacgtgacaccaagtgccagtcacagggctctgtaggagaagagaagagcacctcaacgctcaggggtggggaggttcccgacagaaaaaggcccgagtctgtctactctacttcaaaggacaccaagtaccagtcggtgtatgttctatctgcagaaaaggatgagtgtgttatagcgactgaggttgtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccctgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtcttag BBa_K415508_sequence 1 gctccgaattcgcccttgacagcagagatccagtttggttaattaattggctccggtgcccgtcagtgggcagagcgcacatcgcccacagtccccgagaagttggtgggaggggtcggcaattgaaccggtgcctagagaaggtggcgcggggtaaactgggaaagtgatgtcgtgtactggctccgcctttttcccgagggtgggggagaaccgtatataagtgcagtagtcgccgtgaacgttctttttcgcaacgggtttgccgccagaacacaggtaagtgccgtgtgtggttcccgcgggcctggcctctttacgggttatggcccttgcgtgccttgaattacttccacctggctgcagtacgtgattcttgatcccgagcttcgggttggaagtgggtgggagagttcgaggccttgcgcttaaggagccccttcgcctcgtgcttgagttgaggcctggcctgggcgctggggccgccgcgtgcgaatctggtggcaccttcgcgcctgtctcgctgctttcgataagtctctagccatttaaaatttttgatgacctgctgcgacgctttttttctggcaagatagtcttgtaaatgcgggccaagatctgcacactggtatttcggtttttggggccgcgggcggcgacggggcccgtgcgtcccagcgcacatgttcggcgaggcggggcctgcgagcgcggccaccgagaatcggacgggggtagtctcaagctggccggcctgctctggtgcctggcctcgcgccgccgtgtatcgccccgccctgggcggcaaggctggcccggtcggcaccagttgcgtgagcggaaagatggccgcttcccggccctgctgcagggagctcaaaatggaggacgcggcgctcgggagagcgggcgggtgagtcacccacacaaaggaaaagggcctttccgtcctcagccgtcgcttcatgtgactccacggagtaccgggcgccgtccaggcacctcgattagttctcgagcttttggagtacgtcgtctttaggttggggggaggggttttatgcgatggagtttccccacactgagtgggtggagactgaagttaggccagcttggcacttgatgtaattctccttggaatttgccctttttgagtttggatcttggttcattctcaagcctcagacagtggttcaaagtttttttcttccatttcaggtgtcgtgagaagggcgaattcgac BBa_K511802_sequence 1 gctccgaattcgcccttgacagcagagatccagtttggttaattaattggctccggtgcccgtcagtgggcagagcgcacatcgcccacagtccccgagaagttggtgggaggggtcggcaattgaaccggtgcctagagaaggtggcgcggggtaaactgggaaagtgatgtcgtgtactggctccgcctttttcccgagggtgggggagaaccgtatataagtgcagtagtcgccgtgaacgttctttttcgcaacgggtttgccgccagaacacaggtaagtgccgtgtgtggttcccgcgggcctggcctctttacgggttatggcccttgcgtgccttgaattacttccacctggctgcagtacgtgattcttgatcccgagcttcgggttggaagtgggtgggagagttcgaggccttgcgcttaaggagccccttcgcctcgtgcttgagttgaggcctggcctgggcgctggggccgccgcgtgcgaatctggtggcaccttcgcgcctgtctcgctgctttcgataagtctctagccatttaaaatttttgatgacctgctgcgacgctttttttctggcaagatagtcttgtaaatgcgggccaagatctgcacactggtatttcggtttttggggccgcgggcggcgacggggcccgtgcgtcccagcgcacatgttcggcgaggcggggcctgcgagcgcggccaccgagaatcggacgggggtagtctcaagctggccggcctgctctggtgcctggcctcgcgccgccgtgtatcgccccgccctgggcggcaaggctggcccggtcggcaccagttgcgtgagcggaaagatggccgcttcccggccctgctgcagggagctcaaaatggaggacgcggcgctcgggagagcgggcgggtgagtcacccacacaaaggaaaagggcctttccgtcctcagccgtcgcttcatgtgactccacggagtaccgggcgccgtccaggcacctcgattagttctcgagcttttggagtacgtcgtctttaggttggggggaggggttttatgcgatggagtttccccacactgagtgggtggagactgaagttaggccagcttggcacttgatgtaattctccttggaatttgccctttttgagtttggatcttggttcattctcaagcctcagacagtggttcaaagtttttttcttccatttcaggtgtcgtgagaagggcgaattcgacccaagtttgtacaaaaaagcagatgggccgtcggagcgctctagcccttgccgtggtctcagccctgctgtgccaggtctggagctctggcgtatttgagctgaagctgcaggagttcgtcaacaagaaggggctgctggggaaccgcaactgctgccgcgggggctctggcccgccgtgcgcctgcaggaccttctttcgcgtatgcctcaagcattaccaggccagcgtgtccccggagccaccctgcacctacggcagtgcggtcaccgcagtgctgggtgtcgactccttcagcctgcctgatggcgcaggcatcgaccccgccttcagcaaccccatccgattccccttcggattcacctggccaggtaccttctctctgatcattgaagccctccacacagattctcctgacgacctcgcaacagaaaacccagaaagactcatcagccgcctgaccacacagaggcacctcactgtgggagaagagtggtctcaggaccttcacagtagcggccgcacagacctccgctactcttaccggtttgtgtgtgatgaacactactatggagaaggctgctccgtgttctgccgaccgcgggatgatgcctttggccacttcacctgcggggagagaggggagaagatgtgcgaccctggctggaaaggccagtactgcactgaccccatttgtctgccaggctgtgatgaccaacatggatattgtgacaaaccgggggaatgcaagtgcagagttggctggcagggccgctactgcgatgaatgcatccgatacccaggctgtctccatggtacctgccagcagccctggcagtgtaactgccaggaaggctgggggggcctcttctgcaaccaggatctgaactactgcactcaccataagccatgcaggaacggagccacctgcaccaacacgggccaggggagctacacatgctcttgccgacccgggtatacaggggccaactgtgagctggaggtagatgagtgtgctcccagcccctgcaggaatggagggagctgcacggatcttgaggacagctactcttgcacctgccctcctggcttctatggcaaggtctgtgagctgagcgccatgacgtgtgcagatggtccttgcttcaatgggggacgatgttcggataaccccgatggaggctacacctgccattgccctgcgggcttctctggcttcaactgtgagaagaagattgatctctgtagctcttccccttgttctaacggtgccaagtgtgtggacctcggcaactcctacctgtgccgatgtcagactggcttctccgggaggtactgcgaggacaatgtggatgactgtgcctcttctccctgtgcaaacgggggcacctgccgggacagtgtgaacgatttctcctgtacctgcccacctggctacacaggcaggaactgcagcgcccctgtcagcaggtgtgagcatgcaccctgtcataacggggccacctgccaccagaggggccaacgctacatgtgtgagtgcgcccagggctatggcggcgccaactgccagttcctgctccctgagccaccaccagacctcatagtggcggcccagggcgggtccttcccctgggtggctgtgtgtgccggggtggtgcttgtcctcctgctgctgctgggctgtgctgctgtggtggtctgcgtccggctgaagctacagaaacaccagcctccgcctgatccttgcgggggagagacagagaccatgaacaacctagccaattgccagcgtgagaaggatgtttctgttagcatcattggggctacacagatcaagaacaccaacaagaaggcggactttcatggggaccatggtgctgacaagagcagctttaaggcccgataccccactgtggactataacctcattcgagacctcaagggagatgaagccacggtcagggatgcacacagcaaacgtgacaccaagtgccagtcacagggctctgtaggagaagagaagagcacctcaacgctcaggggtggggaggttcccgacagaaaaaggcccgagtctgtctactctacttcaaaggacaccaagtaccagtcggtgtatgttctatctgcagaaaaggatgagtgtgttatagcgactgaggttgtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccctgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtcttag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z