LOCUS BBa_K511803 4220 bp DNA linear 26-Dec-2024 DEFINITION Repressible Fluorescence/Repression Generator (Hef1a-LacO-AmCyan-mi RFF4) MammoBlock Device ACCESSION BBa_K511803 VERSION BBa_K511803.1 FEATURES Location/Qualifiers promoter 1..1243 /label=BBa_K415508 misc_feature 1245..1265 /label=attB1 misc_feature 1244..1265 /label=BBa_J85600 CDS 1266..3407 /label=Delta-3 Juxtacrine Signaling Ligand CDS 3408..4115 /label=mCherry Fluorescent Protein misc_feature 4116..4118 /label=Stop Codon misc_feature 4127..4214 /label=Four FF4 Binding Sites misc_feature 1266..4220 /label=BBa_K511303 ORIGIN 1 gctccgaatt cgcccttgac agcagagatc cagtttggtt aattaattgg ctccggtgcc 61 cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttggtggg aggggtcggc 121 aattgaaccg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac 181 tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg 241 aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt gtgtggttcc 301 cgcgggcctg gcctctttac gggttatggc ccttgcgtgc cttgaattac ttccacctgg 361 ctgcagtacg tgattcttga tcccgagctt cgggttggaa gtgggtggga gagttcgagg 421 ccttgcgctt aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg 481 ggccgccgcg tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct 541 ctagccattt aaaatttttg atgacctgct gcgacgcttt ttttctggca agatagtctt 601 gtaaatgcgg gccaagatct gcacactggt atttcggttt ttggggccgc gggcggcgac 661 ggggcccgtg cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg 721 agaatcggac gggggtagtc tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg 781 ccgtgtatcg ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg 841 gaaagatggc cgcttcccgg ccctgctgca gggagctcaa aatggaggac gcggcgctcg 901 ggagagcggg cgggtgagtc acccacacaa aggaaaaggg cctttccgtc ctcagccgtc 961 gcttcatgtg actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctcgagc 1021 ttttggagta cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac 1081 actgagtggg tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa 1141 tttgcccttt ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt 1201 ttttttcttc catttcaggt gtcgtgagaa gggcgaattc gacccaagtt tgtacaaaaa 1261 agcagatggg ccgtcggagc gctctagccc ttgccgtggt ctcagccctg ctgtgccagg 1321 tctggagctc tggcgtattt gagctgaagc tgcaggagtt cgtcaacaag aaggggctgc 1381 tggggaaccg caactgctgc cgcgggggct ctggcccgcc gtgcgcctgc aggaccttct 1441 ttcgcgtatg cctcaagcat taccaggcca gcgtgtcccc ggagccaccc tgcacctacg 1501 gcagtgcggt caccgcagtg ctgggtgtcg actccttcag cctgcctgat ggcgcaggca 1561 tcgaccccgc cttcagcaac cccatccgat tccccttcgg attcacctgg ccaggtacct 1621 tctctctgat cattgaagcc ctccacacag attctcctga cgacctcgca acagaaaacc 1681 cagaaagact catcagccgc ctgaccacac agaggcacct cactgtggga gaagagtggt 1741 ctcaggacct tcacagtagc ggccgcacag acctccgcta ctcttaccgg tttgtgtgtg 1801 atgaacacta ctatggagaa ggctgctccg tgttctgccg accgcgggat gatgcctttg 1861 gccacttcac ctgcggggag agaggggaga agatgtgcga ccctggctgg aaaggccagt 1921 actgcactga ccccatttgt ctgccaggct gtgatgacca acatggatat tgtgacaaac 1981 cgggggaatg caagtgcaga gttggctggc agggccgcta ctgcgatgaa tgcatccgat 2041 acccaggctg tctccatggt acctgccagc agccctggca gtgtaactgc caggaaggct 2101 gggggggcct cttctgcaac caggatctga actactgcac tcaccataag ccatgcagga 2161 acggagccac ctgcaccaac acgggccagg ggagctacac atgctcttgc cgacccgggt 2221 atacaggggc caactgtgag ctggaggtag atgagtgtgc tcccagcccc tgcaggaatg 2281 gagggagctg cacggatctt gaggacagct actcttgcac ctgccctcct ggcttctatg 2341 gcaaggtctg tgagctgagc gccatgacgt gtgcagatgg tccttgcttc aatgggggac 2401 gatgttcgga taaccccgat ggaggctaca cctgccattg ccctgcgggc ttctctggct 2461 tcaactgtga gaagaagatt gatctctgta gctcttcccc ttgttctaac ggtgccaagt 2521 gtgtggacct cggcaactcc tacctgtgcc gatgtcagac tggcttctcc gggaggtact 2581 gcgaggacaa tgtggatgac tgtgcctctt ctccctgtgc aaacgggggc acctgccggg 2641 acagtgtgaa cgatttctcc tgtacctgcc cacctggcta cacaggcagg aactgcagcg 2701 cccctgtcag caggtgtgag catgcaccct gtcataacgg ggccacctgc caccagaggg 2761 gccaacgcta catgtgtgag tgcgcccagg gctatggcgg cgccaactgc cagttcctgc 2821 tccctgagcc accaccagac ctcatagtgg cggcccaggg cgggtccttc ccctgggtgg 2881 ctgtgtgtgc cggggtggtg cttgtcctcc tgctgctgct gggctgtgct gctgtggtgg 2941 tctgcgtccg gctgaagcta cagaaacacc agcctccgcc tgatccttgc gggggagaga 3001 cagagaccat gaacaaccta gccaattgcc agcgtgagaa ggatgtttct gttagcatca 3061 ttggggctac acagatcaag aacaccaaca agaaggcgga ctttcatggg gaccatggtg 3121 ctgacaagag cagctttaag gcccgatacc ccactgtgga ctataacctc attcgagacc 3181 tcaagggaga tgaagccacg gtcagggatg cacacagcaa acgtgacacc aagtgccagt 3241 cacagggctc tgtaggagaa gagaagagca cctcaacgct caggggtggg gaggttcccg 3301 acagaaaaag gcccgagtct gtctactcta cttcaaagga caccaagtac cagtcggtgt 3361 atgttctatc tgcagaaaag gatgagtgtg ttatagcgac tgaggttgtg agcaagggcg 3421 aggaggataa catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct 3481 ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca 3541 cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc 3601 tgtcccctca gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg 3661 actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg 3721 acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca 3781 aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca 3841 tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga 3901 tcaagcagag gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct 3961 acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca 4021 tcacctccca caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc 4081 actccaccgg cggcatggac gagctgtaca agtcttaggg cgcgccccgc ttgaagtctt 4141 taattaaacc gcttgaagtc tttaattaaa ccgcttgaag tctttaatta aaccgcttga 4201 agtctttaat taaagctagt //