LOCUS       BBa_K511805             2116 bp    DNA     linear       22-Nov-2024
DEFINITION  Repressible Yellow Fluorescence Generator (Hef1a-LacO-EYFP-4xFF4) 
            MammoBlock Device
ACCESSION   BBa_K511805
VERSION     BBa_K511805.1  
FEATURES             Location/Qualifiers
     promoter        1..1275
                     /label=Hef1-alpha Promoter
     misc_feature    201..221
                     /label=Lac O1
     misc_feature    272..291
                     /label=Lac Oid
     promoter        1..1275
                     /label=BBa_K511000
     misc_feature    1277..1297
                     /label=attB1
     misc_feature    1276..1297
                     /label=BBa_J85600
     CDS             1298..2017
                     /label=Enhanced Yellow Fluorescent Protein
     misc_feature    2029..2116
                     /label=Four FF4 Binding Sites
     misc_feature    1298..2116
                     /label=BBa_K511101
ORIGIN
        1 tggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg
       61 gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag
      121 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc
      181 agtagtcgcc gtgaagctag aattgtgagc ggataacaat tccacagtcg accctaggtt
      241 gtgtcgcgag tgttggatcc cagctgacac caattgtgag cgctcacaat tgctagccgt
      301 tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg
      361 gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca cctggctgca
      421 gtacgtgatt cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg
      481 cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg
      541 ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc
      601 catttaaaat ttttgatgac ctgctgcgac gctttttttc tggcaagata gtcttgtaaa
      661 tgcgggccaa gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc
      721 ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat
      781 cggacggggg tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg
      841 tatcgccccg ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag
      901 atggccgctt cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga
      961 gcgggcgggt gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc
     1021 atgtgactcc acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg
     1081 gagtacgtcg tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga
     1141 gtgggtggag actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc
     1201 cctttttgag tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt
     1261 tcttccattt caggtccaag tttgtacaaa aaagcagatg gtgagcaagg gcgaggagct
     1321 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     1381 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     1441 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tcggctacgg
     1501 cctgcagtgc ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     1561 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     1621 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     1681 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     1741 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
     1801 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     1861 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagctacc agtccaagct
     1921 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     1981 cgggatcact ctcggcatgg acgagctgta caagtaaagc ggcgcgcccc gcttgaagtc
     2041 tttaattaaa ccgcttgaag tctttaatta aaccgcttga agtctttaat taaaccgctt
     2101 gaagtcttta attaaa
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