LOCUS BBa_K511806 4150 bp DNA linear 26-Dec-2024 DEFINITION Repressible Red Fluorescent Ligand Generator (Hef1a-LacO-Delta-mChe rry) MammoBlock Device ACCESSION BBa_K511806 VERSION BBa_K511806.1 FEATURES Location/Qualifiers promoter 1..1275 /label=Hef1-alpha Promoter misc_feature 201..221 /label=Lac O1 misc_feature 272..291 /label=Lac Oid promoter 1..1275 /label=BBa_K511000 misc_feature 1277..1297 /label=attB1 misc_feature 1276..1297 /label=BBa_J85600 CDS 1298..3439 /label=Delta-3 Juxtacrine Signaling Ligand CDS 3440..4147 /label=mCherry Fluorescent Protein misc_feature 4148..4150 /label=Stop Codon misc_feature 1298..4150 /label=BBa_K511302 ORIGIN 1 tggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg 61 gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag 121 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc 181 agtagtcgcc gtgaagctag aattgtgagc ggataacaat tccacagtcg accctaggtt 241 gtgtcgcgag tgttggatcc cagctgacac caattgtgag cgctcacaat tgctagccgt 301 tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg 361 gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca cctggctgca 421 gtacgtgatt cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg 481 cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg 541 ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc 601 catttaaaat ttttgatgac ctgctgcgac gctttttttc tggcaagata gtcttgtaaa 661 tgcgggccaa gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc 721 ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat 781 cggacggggg tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg 841 tatcgccccg ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag 901 atggccgctt cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga 961 gcgggcgggt gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc 1021 atgtgactcc acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg 1081 gagtacgtcg tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga 1141 gtgggtggag actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc 1201 cctttttgag tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt 1261 tcttccattt caggtccaag tttgtacaaa aaagcagatg ggccgtcgga gcgctctagc 1321 ccttgccgtg gtctcagccc tgctgtgcca ggtctggagc tctggcgtat ttgagctgaa 1381 gctgcaggag ttcgtcaaca agaaggggct gctggggaac cgcaactgct gccgcggggg 1441 ctctggcccg ccgtgcgcct gcaggacctt ctttcgcgta tgcctcaagc attaccaggc 1501 cagcgtgtcc ccggagccac cctgcaccta cggcagtgcg gtcaccgcag tgctgggtgt 1561 cgactccttc agcctgcctg atggcgcagg catcgacccc gccttcagca accccatccg 1621 attccccttc ggattcacct ggccaggtac cttctctctg atcattgaag ccctccacac 1681 agattctcct gacgacctcg caacagaaaa cccagaaaga ctcatcagcc gcctgaccac 1741 acagaggcac ctcactgtgg gagaagagtg gtctcaggac cttcacagta gcggccgcac 1801 agacctccgc tactcttacc ggtttgtgtg tgatgaacac tactatggag aaggctgctc 1861 cgtgttctgc cgaccgcggg atgatgcctt tggccacttc acctgcgggg agagagggga 1921 gaagatgtgc gaccctggct ggaaaggcca gtactgcact gaccccattt gtctgccagg 1981 ctgtgatgac caacatggat attgtgacaa accgggggaa tgcaagtgca gagttggctg 2041 gcagggccgc tactgcgatg aatgcatccg atacccaggc tgtctccatg gtacctgcca 2101 gcagccctgg cagtgtaact gccaggaagg ctgggggggc ctcttctgca accaggatct 2161 gaactactgc actcaccata agccatgcag gaacggagcc acctgcacca acacgggcca 2221 ggggagctac acatgctctt gccgacccgg gtatacaggg gccaactgtg agctggaggt 2281 agatgagtgt gctcccagcc cctgcaggaa tggagggagc tgcacggatc ttgaggacag 2341 ctactcttgc acctgccctc ctggcttcta tggcaaggtc tgtgagctga gcgccatgac 2401 gtgtgcagat ggtccttgct tcaatggggg acgatgttcg gataaccccg atggaggcta 2461 cacctgccat tgccctgcgg gcttctctgg cttcaactgt gagaagaaga ttgatctctg 2521 tagctcttcc ccttgttcta acggtgccaa gtgtgtggac ctcggcaact cctacctgtg 2581 ccgatgtcag actggcttct ccgggaggta ctgcgaggac aatgtggatg actgtgcctc 2641 ttctccctgt gcaaacgggg gcacctgccg ggacagtgtg aacgatttct cctgtacctg 2701 cccacctggc tacacaggca ggaactgcag cgcccctgtc agcaggtgtg agcatgcacc 2761 ctgtcataac ggggccacct gccaccagag gggccaacgc tacatgtgtg agtgcgccca 2821 gggctatggc ggcgccaact gccagttcct gctccctgag ccaccaccag acctcatagt 2881 ggcggcccag ggcgggtcct tcccctgggt ggctgtgtgt gccggggtgg tgcttgtcct 2941 cctgctgctg ctgggctgtg ctgctgtggt ggtctgcgtc cggctgaagc tacagaaaca 3001 ccagcctccg cctgatcctt gcgggggaga gacagagacc atgaacaacc tagccaattg 3061 ccagcgtgag aaggatgttt ctgttagcat cattggggct acacagatca agaacaccaa 3121 caagaaggcg gactttcatg gggaccatgg tgctgacaag agcagcttta aggcccgata 3181 ccccactgtg gactataacc tcattcgaga cctcaaggga gatgaagcca cggtcaggga 3241 tgcacacagc aaacgtgaca ccaagtgcca gtcacagggc tctgtaggag aagagaagag 3301 cacctcaacg ctcaggggtg gggaggttcc cgacagaaaa aggcccgagt ctgtctactc 3361 tacttcaaag gacaccaagt accagtcggt gtatgttcta tctgcagaaa aggatgagtg 3421 tgttatagcg actgaggttg tgagcaaggg cgaggaggat aacatggcca tcatcaagga 3481 gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga 3541 gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa 3601 gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa 3661 ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg 3721 cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga 3781 ctcctccctg caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc 3841 ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat 3901 gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg 3961 cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc 4021 cggcgcctac aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat 4081 cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta 4141 caagtcttag //