BBa_K511806
1
BBa_K511806
Repressible Red Fluorescent Ligand Generator (Hef1a-LacO-Delta-mCherry) MammoBlock Device
2011-09-27T11:00:00Z
2015-05-08T01:12:31Z
See constituent parts.
This MammoBlock composite device produces the red fluorescent juxtacrine signaling ligand Delta-mCherry at a low, constitutive level and can be repressed by LacI variants.
false
false
_674_
0
6719
9
Not in stock
false
In order to adhere to the recombination-based MammoBlock standard, and because shipping Biosafety Level 2 mammalian expression vectors to the Registry of Standard Biological Parts is not currently possible, we are providing the sequence resulting from Gateway recombination of the constituent MammoBlock parts here without redistributing the physical DNA plasmids to the Registry. In future years, we hope to make use of improvements in the Registry's accomodations to provide our complete expression vectors.
false
Grant Robinson
component2146047
1
BBa_J85600
component2146051
1
BBa_K511302
component2146045
1
BBa_K511000
annotation2146047
1
BBa_J85600
range2146047
1
1276
1297
annotation2146045
1
BBa_K511000
range2146045
1
1
1275
annotation2146051
1
BBa_K511302
range2146051
1
1298
4150
BBa_J85600
1
BBa_J85600
attB1 recombination site
2011-09-05T11:00:00Z
2015-05-08T01:08:30Z
lambda phage
attB1 recombination site
Used for creating mammoblock (RFC65) composite promoter-gene pairs using recombination-based cloning.
false
false
_406_
0
106
406
Not in stock
false
None
false
Ron Weiss
annotation2125966
1
attB1
range2125966
1
2
22
BBa_K511302
1
BBa_K511302
Delta-mCherry Juxtacrine Signaling Ligand MammoBlock
2011-09-26T11:00:00Z
2015-05-08T01:12:31Z
This part is composed of the Delta-3 ligand from Rattus norvegicus and the synthetic mCherry fluorescent protein engineered from the green fluorescent protein isolated from Aequorea victoria. Additionally, the sequence for this part was included in a plasmid provided to the MIT iGEM 2011 team courtesy of the Elowitz lab at Caltech.
This part encodes a fusion between the Delta-3 ligand and the monomeric mCherry red fluorescent protein.
false
false
_674_
0
6719
9
It's complicated
true
In the original plasmid provided, the Delta-mCherry sequence did not have a stop codon, resulting in plasmid read-through of about nine additional amino acids; our Delta-mCherry includes a stop codon immediately after the mCherry sequence. Both plasmids are phenotypically correct.
false
Grant Robinson
annotation2144604
1
mCherry Fluorescent Protein
range2144604
1
2143
2850
annotation2144623
1
Stop Codon
range2144623
1
2851
2853
annotation2144603
1
Delta-3 Juxtacrine Signaling Ligand
range2144603
1
1
2142
BBa_K511000
1
BBa_K511000
Hef1a-LacOid Promoter MammoBlock
2011-09-24T11:00:00Z
2015-05-08T01:12:31Z
This part is a synthetic promoter formed from the human elongation factor 1a promoter sequence and a LacOid operator that has been computationally shown to bind to the LacI repressor protein more tightly than the wildtype LacO operator.
This part encodes a promoter with low-level, constitutive activity that can be repressed by variants of the LacI transcriptional repressor.
As shown in Figures 1 and 2 below, cotransfection of a construct containing a yellow fluorescent protein (EYFP) driven by the Hef1a-LacO promoter with a construct containing a LacI-KRAB transcriptional repressor driven by the TRE-Tight promoter proves that it is possible to repress Hef1a-LacO expression with the LacI-KRAB LacI variant, which includes a Kruppel-associated box motif previously shown to silence chromatin and thereby inhibit transcription.
false
false
_674_
0
6719
9
It's complicated
true
When sequencing constructs that include this part, it is important to realize that the Hef1a-LacOid promoter is roughly a kilobase long; therefore, promoter-gene pairs will require either internal sequencing primers or sequencing from the gene side of the construct to be useful.
false
Grant Robinson
annotation2139542
1
Lac Oid
range2139542
1
272
291
annotation2139541
1
Lac O1
range2139541
1
201
221
annotation2139540
1
Hef1-alpha Promoter
range2139540
1
1
1275
BBa_J85600_sequence
1
ccaagtttgtacaaaaaagcag
BBa_K511000_sequence
1
tggctccggtgcccgtcagtgggcagagcgcacatcgcccacagtccccgagaagttggggggaggggtcggcaattgaaccggtgcctagagaaggtggcgcggggtaaactgggaaagtgatgtcgtgtactggctccgcctttttcccgagggtgggggagaaccgtatataagtgcagtagtcgccgtgaagctagaattgtgagcggataacaattccacagtcgaccctaggttgtgtcgcgagtgttggatcccagctgacaccaattgtgagcgctcacaattgctagccgttctttttcgcaacgggtttgccgccagaacacaggtaagtgccgtgtgtggttcccgcgggcctggcctctttacgggttatggcccttgcgtgccttgaattacttccacctggctgcagtacgtgattcttgatcccgagcttcgggttggaagtgggtgggagagttcgaggccttgcgcttaaggagccccttcgcctcgtgcttgagttgaggcctggcctgggcgctggggccgccgcgtgcgaatctggtggcaccttcgcgcctgtctcgctgctttcgataagtctctagccatttaaaatttttgatgacctgctgcgacgctttttttctggcaagatagtcttgtaaatgcgggccaagatctgcacactggtatttcggtttttggggccgcgggcggcgacggggcccgtgcgtcccagcgcacatgttcggcgaggcggggcctgcgagcgcggccaccgagaatcggacgggggtagtctcaagctggccggcctgctctggtgcctggcctcgcgccgccgtgtatcgccccgccctgggcggcaaggctggcccggtcggcaccagttgcgtgagcggaaagatggccgcttcccggccctgctgcagggagctcaaaatggaggacgcggcgctcgggagagcgggcgggtgagtcacccacacaaaggaaaagggcctttccgtcctcagccgtcgcttcatgtgactccacggagtaccgggcgccgtccaggcacctcgattagttctcgagcttttggagtacgtcgtctttaggttggggggaggggttttatgcgatggagtttccccacactgagtgggtggagactgaagttaggccagcttggcacttgatgtaattctccttggaatttgccctttttgagtttggatcttggttcattctcaagcctcagacagtggttcaaagtttttttcttccatttcaggt
BBa_K511302_sequence
1
atgggccgtcggagcgctctagcccttgccgtggtctcagccctgctgtgccaggtctggagctctggcgtatttgagctgaagctgcaggagttcgtcaacaagaaggggctgctggggaaccgcaactgctgccgcgggggctctggcccgccgtgcgcctgcaggaccttctttcgcgtatgcctcaagcattaccaggccagcgtgtccccggagccaccctgcacctacggcagtgcggtcaccgcagtgctgggtgtcgactccttcagcctgcctgatggcgcaggcatcgaccccgccttcagcaaccccatccgattccccttcggattcacctggccaggtaccttctctctgatcattgaagccctccacacagattctcctgacgacctcgcaacagaaaacccagaaagactcatcagccgcctgaccacacagaggcacctcactgtgggagaagagtggtctcaggaccttcacagtagcggccgcacagacctccgctactcttaccggtttgtgtgtgatgaacactactatggagaaggctgctccgtgttctgccgaccgcgggatgatgcctttggccacttcacctgcggggagagaggggagaagatgtgcgaccctggctggaaaggccagtactgcactgaccccatttgtctgccaggctgtgatgaccaacatggatattgtgacaaaccgggggaatgcaagtgcagagttggctggcagggccgctactgcgatgaatgcatccgatacccaggctgtctccatggtacctgccagcagccctggcagtgtaactgccaggaaggctgggggggcctcttctgcaaccaggatctgaactactgcactcaccataagccatgcaggaacggagccacctgcaccaacacgggccaggggagctacacatgctcttgccgacccgggtatacaggggccaactgtgagctggaggtagatgagtgtgctcccagcccctgcaggaatggagggagctgcacggatcttgaggacagctactcttgcacctgccctcctggcttctatggcaaggtctgtgagctgagcgccatgacgtgtgcagatggtccttgcttcaatgggggacgatgttcggataaccccgatggaggctacacctgccattgccctgcgggcttctctggcttcaactgtgagaagaagattgatctctgtagctcttccccttgttctaacggtgccaagtgtgtggacctcggcaactcctacctgtgccgatgtcagactggcttctccgggaggtactgcgaggacaatgtggatgactgtgcctcttctccctgtgcaaacgggggcacctgccgggacagtgtgaacgatttctcctgtacctgcccacctggctacacaggcaggaactgcagcgcccctgtcagcaggtgtgagcatgcaccctgtcataacggggccacctgccaccagaggggccaacgctacatgtgtgagtgcgcccagggctatggcggcgccaactgccagttcctgctccctgagccaccaccagacctcatagtggcggcccagggcgggtccttcccctgggtggctgtgtgtgccggggtggtgcttgtcctcctgctgctgctgggctgtgctgctgtggtggtctgcgtccggctgaagctacagaaacaccagcctccgcctgatccttgcgggggagagacagagaccatgaacaacctagccaattgccagcgtgagaaggatgtttctgttagcatcattggggctacacagatcaagaacaccaacaagaaggcggactttcatggggaccatggtgctgacaagagcagctttaaggcccgataccccactgtggactataacctcattcgagacctcaagggagatgaagccacggtcagggatgcacacagcaaacgtgacaccaagtgccagtcacagggctctgtaggagaagagaagagcacctcaacgctcaggggtggggaggttcccgacagaaaaaggcccgagtctgtctactctacttcaaaggacaccaagtaccagtcggtgtatgttctatctgcagaaaaggatgagtgtgttatagcgactgaggttgtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccctgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtcttag
BBa_K511806_sequence
1
tggctccggtgcccgtcagtgggcagagcgcacatcgcccacagtccccgagaagttggggggaggggtcggcaattgaaccggtgcctagagaaggtggcgcggggtaaactgggaaagtgatgtcgtgtactggctccgcctttttcccgagggtgggggagaaccgtatataagtgcagtagtcgccgtgaagctagaattgtgagcggataacaattccacagtcgaccctaggttgtgtcgcgagtgttggatcccagctgacaccaattgtgagcgctcacaattgctagccgttctttttcgcaacgggtttgccgccagaacacaggtaagtgccgtgtgtggttcccgcgggcctggcctctttacgggttatggcccttgcgtgccttgaattacttccacctggctgcagtacgtgattcttgatcccgagcttcgggttggaagtgggtgggagagttcgaggccttgcgcttaaggagccccttcgcctcgtgcttgagttgaggcctggcctgggcgctggggccgccgcgtgcgaatctggtggcaccttcgcgcctgtctcgctgctttcgataagtctctagccatttaaaatttttgatgacctgctgcgacgctttttttctggcaagatagtcttgtaaatgcgggccaagatctgcacactggtatttcggtttttggggccgcgggcggcgacggggcccgtgcgtcccagcgcacatgttcggcgaggcggggcctgcgagcgcggccaccgagaatcggacgggggtagtctcaagctggccggcctgctctggtgcctggcctcgcgccgccgtgtatcgccccgccctgggcggcaaggctggcccggtcggcaccagttgcgtgagcggaaagatggccgcttcccggccctgctgcagggagctcaaaatggaggacgcggcgctcgggagagcgggcgggtgagtcacccacacaaaggaaaagggcctttccgtcctcagccgtcgcttcatgtgactccacggagtaccgggcgccgtccaggcacctcgattagttctcgagcttttggagtacgtcgtctttaggttggggggaggggttttatgcgatggagtttccccacactgagtgggtggagactgaagttaggccagcttggcacttgatgtaattctccttggaatttgccctttttgagtttggatcttggttcattctcaagcctcagacagtggttcaaagtttttttcttccatttcaggtccaagtttgtacaaaaaagcagatgggccgtcggagcgctctagcccttgccgtggtctcagccctgctgtgccaggtctggagctctggcgtatttgagctgaagctgcaggagttcgtcaacaagaaggggctgctggggaaccgcaactgctgccgcgggggctctggcccgccgtgcgcctgcaggaccttctttcgcgtatgcctcaagcattaccaggccagcgtgtccccggagccaccctgcacctacggcagtgcggtcaccgcagtgctgggtgtcgactccttcagcctgcctgatggcgcaggcatcgaccccgccttcagcaaccccatccgattccccttcggattcacctggccaggtaccttctctctgatcattgaagccctccacacagattctcctgacgacctcgcaacagaaaacccagaaagactcatcagccgcctgaccacacagaggcacctcactgtgggagaagagtggtctcaggaccttcacagtagcggccgcacagacctccgctactcttaccggtttgtgtgtgatgaacactactatggagaaggctgctccgtgttctgccgaccgcgggatgatgcctttggccacttcacctgcggggagagaggggagaagatgtgcgaccctggctggaaaggccagtactgcactgaccccatttgtctgccaggctgtgatgaccaacatggatattgtgacaaaccgggggaatgcaagtgcagagttggctggcagggccgctactgcgatgaatgcatccgatacccaggctgtctccatggtacctgccagcagccctggcagtgtaactgccaggaaggctgggggggcctcttctgcaaccaggatctgaactactgcactcaccataagccatgcaggaacggagccacctgcaccaacacgggccaggggagctacacatgctcttgccgacccgggtatacaggggccaactgtgagctggaggtagatgagtgtgctcccagcccctgcaggaatggagggagctgcacggatcttgaggacagctactcttgcacctgccctcctggcttctatggcaaggtctgtgagctgagcgccatgacgtgtgcagatggtccttgcttcaatgggggacgatgttcggataaccccgatggaggctacacctgccattgccctgcgggcttctctggcttcaactgtgagaagaagattgatctctgtagctcttccccttgttctaacggtgccaagtgtgtggacctcggcaactcctacctgtgccgatgtcagactggcttctccgggaggtactgcgaggacaatgtggatgactgtgcctcttctccctgtgcaaacgggggcacctgccgggacagtgtgaacgatttctcctgtacctgcccacctggctacacaggcaggaactgcagcgcccctgtcagcaggtgtgagcatgcaccctgtcataacggggccacctgccaccagaggggccaacgctacatgtgtgagtgcgcccagggctatggcggcgccaactgccagttcctgctccctgagccaccaccagacctcatagtggcggcccagggcgggtccttcccctgggtggctgtgtgtgccggggtggtgcttgtcctcctgctgctgctgggctgtgctgctgtggtggtctgcgtccggctgaagctacagaaacaccagcctccgcctgatccttgcgggggagagacagagaccatgaacaacctagccaattgccagcgtgagaaggatgtttctgttagcatcattggggctacacagatcaagaacaccaacaagaaggcggactttcatggggaccatggtgctgacaagagcagctttaaggcccgataccccactgtggactataacctcattcgagacctcaagggagatgaagccacggtcagggatgcacacagcaaacgtgacaccaagtgccagtcacagggctctgtaggagaagagaagagcacctcaacgctcaggggtggggaggttcccgacagaaaaaggcccgagtctgtctactctacttcaaaggacaccaagtaccagtcggtgtatgttctatctgcagaaaaggatgagtgtgttatagcgactgaggttgtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccctgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtcttag
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z