BBa_K511809 1 BBa_K511809 Repressible Tango Ligand Generator (Hef1a-LacO-pDisplay-Vasopressin-MYC) MammoBlock Device 2011-09-27T11:00:00Z 2015-05-08T01:12:31Z Consult constituent parts. This MammoBlock composite device produces the pDisplay-Vasopressin-MYC synthetic ligand designed to activate expression from the AVPR2-TEVs-Gal4-VP16 signaling receptor. Because this ligand is displayed in a membrane-bound, extracellular fashion, it is ideal for juxtacrine signaling that is orthogonal to other juxtacrine signaling systems, particularly the Notch-Delta system. Transcription is driven by the Hef1a-LacO promoter and therefore repressible by LacI variants. false false _674_ 0 6719 9 Not in stock false In order to adhere to the recombination-based MammoBlock standard, and because shipping Biosafety Level 2 mammalian expression vectors to the Registry of Standard Biological Parts is not currently possible, we are providing the sequence resulting from Gateway recombination of the constituent MammoBlock parts here without redistributing the physical DNA plasmids to the Registry. In future years, we hope to make use of improvements in the Registry's accomodations to provide our complete expression vectors. false Grant Robinson component2146072 1 BBa_J85600 component2146070 1 BBa_K511000 component2146077 1 BBa_K511500 annotation2146070 1 BBa_K511000 range2146070 1 1 1275 annotation2146077 1 BBa_K511500 range2146077 1 1298 1570 annotation2146072 1 BBa_J85600 range2146072 1 1276 1297 BBa_K511000 1 BBa_K511000 Hef1a-LacOid Promoter MammoBlock 2011-09-24T11:00:00Z 2015-05-08T01:12:31Z This part is a synthetic promoter formed from the human elongation factor 1a promoter sequence and a LacOid operator that has been computationally shown to bind to the LacI repressor protein more tightly than the wildtype LacO operator. This part encodes a promoter with low-level, constitutive activity that can be repressed by variants of the LacI transcriptional repressor. As shown in Figures 1 and 2 below, cotransfection of a construct containing a yellow fluorescent protein (EYFP) driven by the Hef1a-LacO promoter with a construct containing a LacI-KRAB transcriptional repressor driven by the TRE-Tight promoter proves that it is possible to repress Hef1a-LacO expression with the LacI-KRAB LacI variant, which includes a Kruppel-associated box motif previously shown to silence chromatin and thereby inhibit transcription. false false _674_ 0 6719 9 It's complicated true When sequencing constructs that include this part, it is important to realize that the Hef1a-LacOid promoter is roughly a kilobase long; therefore, promoter-gene pairs will require either internal sequencing primers or sequencing from the gene side of the construct to be useful. false Grant Robinson annotation2139542 1 Lac Oid range2139542 1 272 291 annotation2139540 1 Hef1-alpha Promoter range2139540 1 1 1275 annotation2139541 1 Lac O1 range2139541 1 201 221 BBa_J85600 1 BBa_J85600 attB1 recombination site 2011-09-05T11:00:00Z 2015-05-08T01:08:30Z lambda phage attB1 recombination site Used for creating mammoblock (RFC65) composite promoter-gene pairs using recombination-based cloning. false false _406_ 0 106 406 Not in stock false None false Ron Weiss annotation2125966 1 attB1 range2125966 1 2 22 BBa_K511500 1 BBa_K511500 pDisplay-Vasopressin-MYC Ligand MammoBlock 2011-09-26T11:00:00Z 2015-05-08T01:12:31Z This protein includes a human codon-optimized version of the arginine vasopressin sequence, the MYC epitope from the human c-MYC DNA-binding transcription factor, and the transmembrane domain from the murine platelet-derived growth factor receptor. This part encodes a novel fusion protein that serves to display the arginine vasopressin hormone in the extracellular space of a mammalian cell. This protein consists of the transmembrane domain of the platelet-derived growth factor receptor, the sequence for arginine vasopressin, and the sequence for the MYC epitope. This synthetic ligand was designed for juxtacrine signaling to the arginine vasopressin receptor (AVPR) class, and was specifically designed to function with the AVPR2-TEVs-Gal4-VP16 construct. false false _674_ 0 6719 9 It's complicated false Not applicable. false Grant Robinson annotation2144663 1 MYC Epitope Tag range2144663 1 91 120 annotation2144664 1 Arginine Vasopressin range2144664 1 64 90 annotation2144662 1 PDGFR Transmembrane Domain range2144662 1 121 270 annotation2144665 1 Murine Ig-Kappa Signal Peptide range2144665 1 1 63 BBa_J85600_sequence 1 ccaagtttgtacaaaaaagcag BBa_K511000_sequence 1 tggctccggtgcccgtcagtgggcagagcgcacatcgcccacagtccccgagaagttggggggaggggtcggcaattgaaccggtgcctagagaaggtggcgcggggtaaactgggaaagtgatgtcgtgtactggctccgcctttttcccgagggtgggggagaaccgtatataagtgcagtagtcgccgtgaagctagaattgtgagcggataacaattccacagtcgaccctaggttgtgtcgcgagtgttggatcccagctgacaccaattgtgagcgctcacaattgctagccgttctttttcgcaacgggtttgccgccagaacacaggtaagtgccgtgtgtggttcccgcgggcctggcctctttacgggttatggcccttgcgtgccttgaattacttccacctggctgcagtacgtgattcttgatcccgagcttcgggttggaagtgggtgggagagttcgaggccttgcgcttaaggagccccttcgcctcgtgcttgagttgaggcctggcctgggcgctggggccgccgcgtgcgaatctggtggcaccttcgcgcctgtctcgctgctttcgataagtctctagccatttaaaatttttgatgacctgctgcgacgctttttttctggcaagatagtcttgtaaatgcgggccaagatctgcacactggtatttcggtttttggggccgcgggcggcgacggggcccgtgcgtcccagcgcacatgttcggcgaggcggggcctgcgagcgcggccaccgagaatcggacgggggtagtctcaagctggccggcctgctctggtgcctggcctcgcgccgccgtgtatcgccccgccctgggcggcaaggctggcccggtcggcaccagttgcgtgagcggaaagatggccgcttcccggccctgctgcagggagctcaaaatggaggacgcggcgctcgggagagcgggcgggtgagtcacccacacaaaggaaaagggcctttccgtcctcagccgtcgcttcatgtgactccacggagtaccgggcgccgtccaggcacctcgattagttctcgagcttttggagtacgtcgtctttaggttggggggaggggttttatgcgatggagtttccccacactgagtgggtggagactgaagttaggccagcttggcacttgatgtaattctccttggaatttgccctttttgagtttggatcttggttcattctcaagcctcagacagtggttcaaagtttttttcttccatttcaggt BBa_K511809_sequence 1 tggctccggtgcccgtcagtgggcagagcgcacatcgcccacagtccccgagaagttggggggaggggtcggcaattgaaccggtgcctagagaaggtggcgcggggtaaactgggaaagtgatgtcgtgtactggctccgcctttttcccgagggtgggggagaaccgtatataagtgcagtagtcgccgtgaagctagaattgtgagcggataacaattccacagtcgaccctaggttgtgtcgcgagtgttggatcccagctgacaccaattgtgagcgctcacaattgctagccgttctttttcgcaacgggtttgccgccagaacacaggtaagtgccgtgtgtggttcccgcgggcctggcctctttacgggttatggcccttgcgtgccttgaattacttccacctggctgcagtacgtgattcttgatcccgagcttcgggttggaagtgggtgggagagttcgaggccttgcgcttaaggagccccttcgcctcgtgcttgagttgaggcctggcctgggcgctggggccgccgcgtgcgaatctggtggcaccttcgcgcctgtctcgctgctttcgataagtctctagccatttaaaatttttgatgacctgctgcgacgctttttttctggcaagatagtcttgtaaatgcgggccaagatctgcacactggtatttcggtttttggggccgcgggcggcgacggggcccgtgcgtcccagcgcacatgttcggcgaggcggggcctgcgagcgcggccaccgagaatcggacgggggtagtctcaagctggccggcctgctctggtgcctggcctcgcgccgccgtgtatcgccccgccctgggcggcaaggctggcccggtcggcaccagttgcgtgagcggaaagatggccgcttcccggccctgctgcagggagctcaaaatggaggacgcggcgctcgggagagcgggcgggtgagtcacccacacaaaggaaaagggcctttccgtcctcagccgtcgcttcatgtgactccacggagtaccgggcgccgtccaggcacctcgattagttctcgagcttttggagtacgtcgtctttaggttggggggaggggttttatgcgatggagtttccccacactgagtgggtggagactgaagttaggccagcttggcacttgatgtaattctccttggaatttgccctttttgagtttggatcttggttcattctcaagcctcagacagtggttcaaagtttttttcttccatttcaggtccaagtttgtacaaaaaagcagatggagacagacacactcctgctatgggtactgctgctctgggttccaggttccactggtgactcctacttccagaactctcccagcggcgaacaaaaactcatctcagaagaggatctgaatgctgtgggccaggacacgcaggaggtcatcgtggtgccacactccttgccctttaaggtggtggtgatctcagccatcctggccctggtggtgctcaccatcatctcccttatcatcctcatcatgctttggcagaagaagccacgttag BBa_K511500_sequence 1 atggagacagacacactcctgctatgggtactgctgctctgggttccaggttccactggtgactcctacttccagaactctcccagcggcgaacaaaaactcatctcagaagaggatctgaatgctgtgggccaggacacgcaggaggtcatcgtggtgccacactccttgccctttaaggtggtggtgatctcagccatcctggccctggtggtgctcaccatcatctcccttatcatcctcatcatgctttggcagaagaagccacgttag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z