LOCUS BBa_K511811 6706 bp DNA linear 26-Dec-2024 DEFINITION Notch-Gal4-ESN Juxtacrine Receptor Generator (CMV-Notch-Gal4-ESN) MammoBlock Device ACCESSION BBa_K511811 VERSION BBa_K511811.1 FEATURES Location/Qualifiers promoter 1..609 /label=BBa_K415514 misc_feature 611..631 /label=attB1 misc_feature 610..631 /label=BBa_J85600 CDS 632..5854 /label=Notch-1 Extracellular Domain misc_feature 5855..5920 /label=Transmembrane Linker CDS 5921..6703 /label=Gal4-ESN Minimal Transactivation Domain misc_feature 632..6706 /label=BBa_K511300 ORIGIN 1 gctccgaatt ctagttatta atagtaatca attacggggt cattagttca tagcccatat 61 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac 121 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc 181 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg 241 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat 301 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc 361 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt 421 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac 481 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc 541 ggtaggcgtg tacggtggga ggtctatata agcagagctg gtttagtgaa ccgtcagatc 601 gaattcgacc caagtttgta caaaaaagca gatgccgccg ctcctggcgc ccctgctctg 661 cctggcgctg ctgcccgcgc tcgccgcacg aggcccgcga tgctcccagc ccggtgagac 721 ctgcctgaat ggcgggaagt gtgaagcggc caatggcacg gaggcctgcg tctgtggcgg 781 ggccttcgtg ggcccgcgat gccaggaccc caacccgtgc ctcagcaccc cctgcaagaa 841 cgccgggaca tgccacgtgg tggaccgcgg cagactatgc ctgcagctgt gccctgggct 901 tctctgggcc cctctgcctg acacccctgg acaatgcctg cctcaccaac ccctgccgca 961 acgggggcac ctgcgacctg ctcacgctga cggagtacaa gtgccgctgc ccgcccggct 1021 ggtcagggaa atcgtgccag caggctgacc cgtgcgcctc caacccctgc gccaacggtg 1081 gccagtgcct gcccttcgag gcctcctaca tctgccactg cccacccagc ttccatggcc 1141 ccacctgccg gcaggatgtc aacgagtgtg gccagaagcc cgggctttgc cgccacggag 1201 gcacctgcca caacgaggtc ggctcctacc gctgcgtctg ccgcgccacc cacactggcc 1261 ccaactgcga gcggccctac gtgccctgca gcccctcgcc ctgccagaac gggggcacct 1321 gccgccccac gggcgacgtc acccacgagt gtgcctgcct gccaggcttc accggccaga 1381 actgtgagga aaatatcgac gattgtccag gaaacaactg caagaacggg ggtgcctgtg 1441 tggacggcgt gaacacctac aactgccgct gcccgccaga gtggacaggt cagtactgta 1501 ccgaggatgt ggacgagtgc cagctgatgc caaatgcctg ccagaacggc gggacctgcc 1561 acaacaccca cggtggctac aactgcgtgt gtgtcaacgg ctggactggt gaggactgca 1621 gcgagaacat tgatgactgt gccagcgccg cctgcttcca cggcgccacc tgccatgacc 1681 gtgtggcctc cttctactgc gagtgtcccc atggccgcac aggtctgctg tgccacctca 1741 acgacgcatg catcagcaac ccctgtaacg agggctccaa ctgcgacacc aaccctgtca 1801 atggcaaggc catctgcacc tgcccctcgg ggtacacggg cccggcctgc agccaggacg 1861 tggatgagtg ctcgctgggt gccaacccct gcgagcatgc gggcaagtgc atcaacacgc 1921 tgggctcctt cgagtgccag tgtctgcagg gctacacggg cccccgatgc gagatcgacg 1981 tcaacgagtg cgtctcgaac ccgtgccaga acgacgccac ctgcctggac cagattgggg 2041 agttccagtg catctgcatg cccggctacg agggtgtgca ctgcgaggtc aacacagacg 2101 agtgtgccag cagcccctgc ctgcacaatg gccgctgcct ggacaagatc aatgagttcc 2161 agtgcgagtg ccccacgggc ttcactgggc atctgtgcca gtacgatgtg gacgagtgtg 2221 ccagcacccc ctgcaagaat ggtgccaagt gcctggacgg acccaacact tacacctgtg 2281 tgtgcacgga agggtacacg gggacgcact gcgaggtgga catcgatgag tgcgaccccg 2341 acccctgcca ctacggctcc tgcaaggacg gcgtcgccac cttcacctgc ctctgccgcc 2401 caggctacac gggccaccac tgcgagacca acatcaacga gtgctccagc cagccctgcc 2461 gccacggggg cacctgccag gaccgcgaca acgcctacct ctgcttctgc ctgaagggga 2521 ccacaggacc caactgcgag atcaacctgg atgactgtgc cagcagcccc tgcgactcgg 2581 gcacctgtct ggacaagatc gatggctacg agtgtgcctg tgagccgggc tacacaggga 2641 gcatgtgtaa catcaacatc gatgagtgtg cgggcaaccc ctgccacaac gggggcacct 2701 gcgaggacgg catcaatggc ttcacctgcc gctgccccga gggctaccac gaccccacct 2761 gcctgtctga ggtcaatgag tgcaacagca acccctgcgt ccacggggcc tgccgggaca 2821 gcctcaacgg gtacaagtgc gactgtgacc ctgggtggag tgggaccaac tgtgacatca 2881 acaacaatga gtgtgaatcc aacccttgtg tcaacggcgg cacctgcaaa gacatgacca 2941 gtggctacgt gtgcacctgc cgggagggct tcagcggtcc caactgccag accaacatca 3001 acgagtgtgc gtccaaccca tgtctgaacc agggcacgtg tattgacgac gttgccgggt 3061 acaagtgcaa ctgcctgctg ccctacacag gtgccacgtg tgaggtggtg ctggccccgt 3121 gtgcccccag cccctgcaga aacggcgggg agtgcaggca atccgaggac tatgagagct 3181 tctcctgtgt ctgccccacg ggctggcaag cagggcagac ctgtgaggtc gacatcaacg 3241 agtgcgttct gagcccgtgc cggcacggcg catcctgcca gaacacccac ggcggctacc 3301 gctgccactg ccaggccggc tacagtgggc gcaactgcga gaccgacatc gacgactgcc 3361 ggcccaaccc gtgtcacaac gggggctcct gcacagacgg catcaacacg gccttctgcg 3421 actgcctgcc cggcttccgg ggcactttct gtgaggagga catcaacgag tgtgccagtg 3481 acccctgccg caacggggcc aactgcacgg actgcgtgga cagctacacg tgcacctgcc 3541 ccgcaggctt cagcgggatc cactgtgaga acaacacgcc tgactgcaca gagagctcct 3601 gcttcaacgg tggcacctgc gtggacggca tcaactcgtt cacctgcctg tgtccacccg 3661 gcttcacggg cagctactgc cagcacgatg tcaatgagtg cgactcacag ccctgcctgc 3721 atggcggcac ctgtcaggac ggctgcggct cctacaggtg cacctgcccc cagggctaca 3781 ctggccccaa ctgccagaac cttgtgcact ggtgtgactc ctcgccctgc aagaacggcg 3841 gcaaatgctg gcagacccac acccagtacc gctgcgagtg ccccagcggc tggaccggcc 3901 tttactgcga cgtgcccagc gtgtcctgtg aggtggctgc gcagcgacaa ggtgttgacg 3961 ttgcccgcct gtgccagcat ggagggctct gtgtggacgc gggcaacacg caccactgcc 4021 gctgccaggc gggctacaca ggcagctact gtgaggacct ggtggacgag tgctcaccca 4081 gcccctgcca gaacggggcc acctgcacgg actacctggg cggctactcc tgcaagtgcg 4141 tggccggcta ccacggggtg aactgctctg aggagatcga cgagtgcctc tcccacccct 4201 gccagaacgg gggcacctgc ctcgacctcc ccaacaccta caagtgctcc tgcccacggg 4261 gcactcaggg tgtgcactgt gagatcaacg tggacgactg caatcccccc gttgaccccg 4321 tgtcccggag ccccaagtgc tttaacaacg gcacctgcgt ggaccaggtg ggcggctaca 4381 gctgcacctg cccgccgggc ttcgtgggtg agcgctgtga gggggatgtc aacgagtgcc 4441 tgtccaatcc ctgcgacgcc cgtggcaccc agaactgcgt gcagcgcgtc aatgacttcc 4501 actgcgagtg ccgtgctggt cacaccgggc gccgctgcga gtccgtcatc aatggctgca 4561 aaggcaagcc ctgcaagaat gggggcacct gcgccgtggc ctccaacacc gcccgcgggt 4621 tcatctgcaa gtgccctgcg ggcttcgagg gcgccacgtg tgagaatgac gctcgtacct 4681 gcggcagcct gcgctgcctc aacggcggca catgcatctc cggcccgcgc agccccacct 4741 gcctgtgcct gggccccttc acgggccccg aatgccagtt cccggccagc agcccctgcc 4801 tgggcggcaa cccctgctac aaccagggga cctgtgagcc cacatccgag agccccttct 4861 accgttgcct gtgccccgcc aaattcaacg ggctcttgtg ccacatcctg gactacagct 4921 tcgggggtgg ggccgggcgc gacatccccc cgccgctgat cgaggaggcg tgcgagctgc 4981 ccgagtgcca ggaggacgcg ggcaacaagg tctgcagcct gcagtgcaac aaccacgcgt 5041 gcggctggga cggcggtgac tgctccctca acttcaatga cccctggaag aactgcacgc 5101 agtctctgca gtgctggaag tacttcagtg acggccactg tgacagccag tgcaactcag 5161 ccggctgcct cttcgacggc tttgactgcc agcgtgcgga aggccagtgc aaccccctgt 5221 acgaccagta ctgcaaggac cacttcagcg acgggcactg cgaccagggc tgcaacagcg 5281 cggagtgcga gtgggacggg ctggactgtg cggagcatgt acccgagagg ctggcggccg 5341 gcacgctggt ggtggtggtg ctgatgccgc cggagcagct gcgcaacagc tccttccact 5401 tcctgcggga gctcagccgc gtgctgcaca ccaacgtggt cttcaagcgt gacgcacacg 5461 gccagcagat gatcttcccc tactacggcc gcgaggagga gctgcgcaag caccccatca 5521 agcgtgccgc cgagggctgg gccgcacctg acgccctgct gggccaggtg aaggcctcgc 5581 tgctccctgg tggcagcgag ggtgggcggc ggcggaggga gctggacccc atggacgtcc 5641 gcggctccat cgtctacctg gagattgaca accggcagtg tgtgcaggcc tcctcgcagt 5701 gcttccagag tgccaccgac gtggccgcat tcctgggagc gctcgcctcg ctgggcagcc 5761 tcaacatccc ctacaagatc gaggccgtgc agagtgagac cgtggagccg cccccgccgg 5821 cgcagctgca cttcatgtac gtggcggcgg ccgcgcggcc gcctttgtgc ttctgttctt 5881 cgtgggctgc ggggtgctgc tgtcccgcaa gcgccggcgg atgaagctac tgtcttctat 5941 cgaacaagca tgcgatattt gccgacttaa aaagctcaag tgctccaaag aaaaaccgaa 6001 gtgcgccaag tgtctgaaga acaactggga gtgtcgctac tctcccaaaa ccaaaaggtc 6061 tccgctgact agggcacatc tgacagaagt ggaatcaagg ctagaaagac tggaacagct 6121 atttctactg atttttcctc gagaagacct tgacatgatt ttgaaaatgg attctttaca 6181 ggatataaaa gcattgttaa caggattatt tgtacaagat aatgtgaata aagatgccgt 6241 cacagataga ttggcttcag tggagactga tatgcctcta acattgagac agcatagaat 6301 aagtgcgaca tcatcatcgg aagagagtag taacaaaggt caaagacagt tgactgtatc 6361 ggccaatttt aatcaaagtg ggaatattgc tgatagctca ttgtccttca ctttcactaa 6421 cagtagcaac ggtccgaacc tcataacaac tcaaacaaat tctcaagcgc tttcacaacc 6481 aattgcctcc tctaacgttc atgataactt catgaataat gaaatcacgg ctagtaaaat 6541 tgatgatggt aataattcaa aaccactgtc acctggttgg acggaccaaa ctgcgtataa 6601 cgcgtttgga atcactacag ggatgtttaa taccactaca atggatgatg tatataacta 6661 tctattcgat gatgaagata ccccaccaaa cccaaaaaaa gagtaa //