LOCUS       BBa_K511814             3205 bp    DNA     linear       22-Dec-2024
DEFINITION  Inducible Red Fluorescent Ligand Generator (TRE-Tight-Delta-mCherry
            ) MammoBlock Device
ACCESSION   BBa_K511814
VERSION     BBa_K511814.1  
FEATURES             Location/Qualifiers
     misc_feature    46..63
                     /label=TetO Site1
     misc_feature    81..98
                     /label=TetO Site2
     misc_feature    117..132
                     /label=TetO Site3
     misc_feature    153..170
                     /label=TetO Site4
     misc_feature    188..205
                     /label=TetO Site5
     misc_feature    224..241
                     /label=TetO Site6
     promoter        1..330
                     /label=BBa_K415506
     misc_feature    332..352
                     /label=attB1
     misc_feature    331..352
                     /label=BBa_J85600
     CDS             353..2494
                     /label=Delta-3 Juxtacrine Signaling Ligand
     CDS             2495..3202
                     /label=mCherry Fluorescent Protein
     misc_feature    3203..3205
                     /label=Stop Codon
     misc_feature    353..3205
                     /label=BBa_K511302
ORIGIN
        1 gctccgaatt cgcccttcag gtccgaggtt ctagacgagt ttactcccta tcagtgatag
       61 agaacgatgt cgagtttact ccctatcagt gatagagaac gtatgtcgag tttactccct
      121 atcagtgata gagaacgtat gtcgagttta ctccctatca gtgatagaga acgtatgtcg
      181 agtttatccc tatcagtgat agagaacgta tgtcgagttt actccctatc agtgatagag
      241 aacgtatgtc gaggtaggcg tgtacggtgg gaggcctata taagcagagc tcgtttagtg
      301 aaccgtcaga tcgcaaaggg cgaattcgac ccaagtttgt acaaaaaagc agatgggccg
      361 tcggagcgct ctagcccttg ccgtggtctc agccctgctg tgccaggtct ggagctctgg
      421 cgtatttgag ctgaagctgc aggagttcgt caacaagaag gggctgctgg ggaaccgcaa
      481 ctgctgccgc gggggctctg gcccgccgtg cgcctgcagg accttctttc gcgtatgcct
      541 caagcattac caggccagcg tgtccccgga gccaccctgc acctacggca gtgcggtcac
      601 cgcagtgctg ggtgtcgact ccttcagcct gcctgatggc gcaggcatcg accccgcctt
      661 cagcaacccc atccgattcc ccttcggatt cacctggcca ggtaccttct ctctgatcat
      721 tgaagccctc cacacagatt ctcctgacga cctcgcaaca gaaaacccag aaagactcat
      781 cagccgcctg accacacaga ggcacctcac tgtgggagaa gagtggtctc aggaccttca
      841 cagtagcggc cgcacagacc tccgctactc ttaccggttt gtgtgtgatg aacactacta
      901 tggagaaggc tgctccgtgt tctgccgacc gcgggatgat gcctttggcc acttcacctg
      961 cggggagaga ggggagaaga tgtgcgaccc tggctggaaa ggccagtact gcactgaccc
     1021 catttgtctg ccaggctgtg atgaccaaca tggatattgt gacaaaccgg gggaatgcaa
     1081 gtgcagagtt ggctggcagg gccgctactg cgatgaatgc atccgatacc caggctgtct
     1141 ccatggtacc tgccagcagc cctggcagtg taactgccag gaaggctggg ggggcctctt
     1201 ctgcaaccag gatctgaact actgcactca ccataagcca tgcaggaacg gagccacctg
     1261 caccaacacg ggccagggga gctacacatg ctcttgccga cccgggtata caggggccaa
     1321 ctgtgagctg gaggtagatg agtgtgctcc cagcccctgc aggaatggag ggagctgcac
     1381 ggatcttgag gacagctact cttgcacctg ccctcctggc ttctatggca aggtctgtga
     1441 gctgagcgcc atgacgtgtg cagatggtcc ttgcttcaat gggggacgat gttcggataa
     1501 ccccgatgga ggctacacct gccattgccc tgcgggcttc tctggcttca actgtgagaa
     1561 gaagattgat ctctgtagct cttccccttg ttctaacggt gccaagtgtg tggacctcgg
     1621 caactcctac ctgtgccgat gtcagactgg cttctccggg aggtactgcg aggacaatgt
     1681 ggatgactgt gcctcttctc cctgtgcaaa cgggggcacc tgccgggaca gtgtgaacga
     1741 tttctcctgt acctgcccac ctggctacac aggcaggaac tgcagcgccc ctgtcagcag
     1801 gtgtgagcat gcaccctgtc ataacggggc cacctgccac cagaggggcc aacgctacat
     1861 gtgtgagtgc gcccagggct atggcggcgc caactgccag ttcctgctcc ctgagccacc
     1921 accagacctc atagtggcgg cccagggcgg gtccttcccc tgggtggctg tgtgtgccgg
     1981 ggtggtgctt gtcctcctgc tgctgctggg ctgtgctgct gtggtggtct gcgtccggct
     2041 gaagctacag aaacaccagc ctccgcctga tccttgcggg ggagagacag agaccatgaa
     2101 caacctagcc aattgccagc gtgagaagga tgtttctgtt agcatcattg gggctacaca
     2161 gatcaagaac accaacaaga aggcggactt tcatggggac catggtgctg acaagagcag
     2221 ctttaaggcc cgatacccca ctgtggacta taacctcatt cgagacctca agggagatga
     2281 agccacggtc agggatgcac acagcaaacg tgacaccaag tgccagtcac agggctctgt
     2341 aggagaagag aagagcacct caacgctcag gggtggggag gttcccgaca gaaaaaggcc
     2401 cgagtctgtc tactctactt caaaggacac caagtaccag tcggtgtatg ttctatctgc
     2461 agaaaaggat gagtgtgtta tagcgactga ggttgtgagc aagggcgagg aggataacat
     2521 ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca
     2581 cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa
     2641 gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt
     2701 catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct
     2761 gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt
     2821 gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg
     2881 cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc
     2941 ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct
     3001 gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa
     3061 gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa
     3121 cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg
     3181 catggacgag ctgtacaagt cttag
//