BBa_K415506 1 BBa_K415506 pTRE-Tight L4R1 MammoBlock 2010-10-26T11:00:00Z 2015-05-08T01:12:27Z Source: modified TRE promoter. This inducible promoter can be switched on by the activity of rttA3 transcription factor. It has been optimized to contain multiple TRE binding sites; this makes the 'tight' version a significant improvement over the previously used simple TRE promoter. false true _525_ 0 6768 9 It's complicated false Increased number of TRE operator binding sites. false Laura Deming, Joy Jiao, Adrian Slusarczyk annotation2109714 1 TetO Site5 range2109714 1 188 205 annotation2109711 1 TetO Site2 range2109711 1 81 98 annotation2109712 1 TetO Site3 range2109712 1 117 132 annotation2109715 1 TetO Site6 range2109715 1 224 241 annotation2109713 1 TetO Site4 range2109713 1 153 170 annotation2109710 1 TetO Site1 range2109710 1 46 63 BBa_J85600 1 BBa_J85600 attB1 recombination site 2011-09-05T11:00:00Z 2015-05-08T01:08:30Z lambda phage attB1 recombination site Used for creating mammoblock (RFC65) composite promoter-gene pairs using recombination-based cloning. false false _406_ 0 106 406 Not in stock false None false Ron Weiss annotation2125966 1 attB1 range2125966 1 2 22 BBa_K511814 1 BBa_K511814 Inducible Red Fluorescent Ligand Generator (TRE-Tight-Delta-mCherry) MammoBlock Device 2011-09-27T11:00:00Z 2015-05-08T01:12:31Z Consult constituent components. This MammoBlock composite device produces the red fluorescent juxtacrine signaling ligand Delta-mCherry when induced with tTA/rtTA transactivator variants in the presence of tetracycline analogues, and otherwise produces Delta-mCherry at a low, basal (OFF) level. false false _674_ 0 6719 9 Not in stock false In order to adhere to the recombination-based MammoBlock standard, and because shipping Biosafety Level 2 mammalian expression vectors to the Registry of Standard Biological Parts is not currently possible, we are providing the sequence resulting from Gateway recombination of the constituent MammoBlock parts here without redistributing the physical DNA plasmids to the Registry. In future years, we hope to make use of improvements in the Registry's accommodations to provide our complete expression vectors. false Grant Robinson component2146183 1 BBa_J85600 component2146181 1 BBa_K415506 component2146187 1 BBa_K511302 annotation2146187 1 BBa_K511302 range2146187 1 353 3205 annotation2146181 1 BBa_K415506 range2146181 1 1 330 annotation2146183 1 BBa_J85600 range2146183 1 331 352 BBa_K511302 1 BBa_K511302 Delta-mCherry Juxtacrine Signaling Ligand MammoBlock 2011-09-26T11:00:00Z 2015-05-08T01:12:31Z This part is composed of the Delta-3 ligand from Rattus norvegicus and the synthetic mCherry fluorescent protein engineered from the green fluorescent protein isolated from Aequorea victoria. Additionally, the sequence for this part was included in a plasmid provided to the MIT iGEM 2011 team courtesy of the Elowitz lab at Caltech. This part encodes a fusion between the Delta-3 ligand and the monomeric mCherry red fluorescent protein. false false _674_ 0 6719 9 It's complicated true In the original plasmid provided, the Delta-mCherry sequence did not have a stop codon, resulting in plasmid read-through of about nine additional amino acids; our Delta-mCherry includes a stop codon immediately after the mCherry sequence. Both plasmids are phenotypically correct. false Grant Robinson annotation2144623 1 Stop Codon range2144623 1 2851 2853 annotation2144604 1 mCherry Fluorescent Protein range2144604 1 2143 2850 annotation2144603 1 Delta-3 Juxtacrine Signaling Ligand range2144603 1 1 2142 BBa_J85600_sequence 1 ccaagtttgtacaaaaaagcag BBa_K511814_sequence 1 gctccgaattcgcccttcaggtccgaggttctagacgagtttactccctatcagtgatagagaacgatgtcgagtttactccctatcagtgatagagaacgtatgtcgagtttactccctatcagtgatagagaacgtatgtcgagtttactccctatcagtgatagagaacgtatgtcgagtttatccctatcagtgatagagaacgtatgtcgagtttactccctatcagtgatagagaacgtatgtcgaggtaggcgtgtacggtgggaggcctatataagcagagctcgtttagtgaaccgtcagatcgcaaagggcgaattcgacccaagtttgtacaaaaaagcagatgggccgtcggagcgctctagcccttgccgtggtctcagccctgctgtgccaggtctggagctctggcgtatttgagctgaagctgcaggagttcgtcaacaagaaggggctgctggggaaccgcaactgctgccgcgggggctctggcccgccgtgcgcctgcaggaccttctttcgcgtatgcctcaagcattaccaggccagcgtgtccccggagccaccctgcacctacggcagtgcggtcaccgcagtgctgggtgtcgactccttcagcctgcctgatggcgcaggcatcgaccccgccttcagcaaccccatccgattccccttcggattcacctggccaggtaccttctctctgatcattgaagccctccacacagattctcctgacgacctcgcaacagaaaacccagaaagactcatcagccgcctgaccacacagaggcacctcactgtgggagaagagtggtctcaggaccttcacagtagcggccgcacagacctccgctactcttaccggtttgtgtgtgatgaacactactatggagaaggctgctccgtgttctgccgaccgcgggatgatgcctttggccacttcacctgcggggagagaggggagaagatgtgcgaccctggctggaaaggccagtactgcactgaccccatttgtctgccaggctgtgatgaccaacatggatattgtgacaaaccgggggaatgcaagtgcagagttggctggcagggccgctactgcgatgaatgcatccgatacccaggctgtctccatggtacctgccagcagccctggcagtgtaactgccaggaaggctgggggggcctcttctgcaaccaggatctgaactactgcactcaccataagccatgcaggaacggagccacctgcaccaacacgggccaggggagctacacatgctcttgccgacccgggtatacaggggccaactgtgagctggaggtagatgagtgtgctcccagcccctgcaggaatggagggagctgcacggatcttgaggacagctactcttgcacctgccctcctggcttctatggcaaggtctgtgagctgagcgccatgacgtgtgcagatggtccttgcttcaatgggggacgatgttcggataaccccgatggaggctacacctgccattgccctgcgggcttctctggcttcaactgtgagaagaagattgatctctgtagctcttccccttgttctaacggtgccaagtgtgtggacctcggcaactcctacctgtgccgatgtcagactggcttctccgggaggtactgcgaggacaatgtggatgactgtgcctcttctccctgtgcaaacgggggcacctgccgggacagtgtgaacgatttctcctgtacctgcccacctggctacacaggcaggaactgcagcgcccctgtcagcaggtgtgagcatgcaccctgtcataacggggccacctgccaccagaggggccaacgctacatgtgtgagtgcgcccagggctatggcggcgccaactgccagttcctgctccctgagccaccaccagacctcatagtggcggcccagggcgggtccttcccctgggtggctgtgtgtgccggggtggtgcttgtcctcctgctgctgctgggctgtgctgctgtggtggtctgcgtccggctgaagctacagaaacaccagcctccgcctgatccttgcgggggagagacagagaccatgaacaacctagccaattgccagcgtgagaaggatgtttctgttagcatcattggggctacacagatcaagaacaccaacaagaaggcggactttcatggggaccatggtgctgacaagagcagctttaaggcccgataccccactgtggactataacctcattcgagacctcaagggagatgaagccacggtcagggatgcacacagcaaacgtgacaccaagtgccagtcacagggctctgtaggagaagagaagagcacctcaacgctcaggggtggggaggttcccgacagaaaaaggcccgagtctgtctactctacttcaaaggacaccaagtaccagtcggtgtatgttctatctgcagaaaaggatgagtgtgttatagcgactgaggttgtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccctgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtcttag BBa_K511302_sequence 1 atgggccgtcggagcgctctagcccttgccgtggtctcagccctgctgtgccaggtctggagctctggcgtatttgagctgaagctgcaggagttcgtcaacaagaaggggctgctggggaaccgcaactgctgccgcgggggctctggcccgccgtgcgcctgcaggaccttctttcgcgtatgcctcaagcattaccaggccagcgtgtccccggagccaccctgcacctacggcagtgcggtcaccgcagtgctgggtgtcgactccttcagcctgcctgatggcgcaggcatcgaccccgccttcagcaaccccatccgattccccttcggattcacctggccaggtaccttctctctgatcattgaagccctccacacagattctcctgacgacctcgcaacagaaaacccagaaagactcatcagccgcctgaccacacagaggcacctcactgtgggagaagagtggtctcaggaccttcacagtagcggccgcacagacctccgctactcttaccggtttgtgtgtgatgaacactactatggagaaggctgctccgtgttctgccgaccgcgggatgatgcctttggccacttcacctgcggggagagaggggagaagatgtgcgaccctggctggaaaggccagtactgcactgaccccatttgtctgccaggctgtgatgaccaacatggatattgtgacaaaccgggggaatgcaagtgcagagttggctggcagggccgctactgcgatgaatgcatccgatacccaggctgtctccatggtacctgccagcagccctggcagtgtaactgccaggaaggctgggggggcctcttctgcaaccaggatctgaactactgcactcaccataagccatgcaggaacggagccacctgcaccaacacgggccaggggagctacacatgctcttgccgacccgggtatacaggggccaactgtgagctggaggtagatgagtgtgctcccagcccctgcaggaatggagggagctgcacggatcttgaggacagctactcttgcacctgccctcctggcttctatggcaaggtctgtgagctgagcgccatgacgtgtgcagatggtccttgcttcaatgggggacgatgttcggataaccccgatggaggctacacctgccattgccctgcgggcttctctggcttcaactgtgagaagaagattgatctctgtagctcttccccttgttctaacggtgccaagtgtgtggacctcggcaactcctacctgtgccgatgtcagactggcttctccgggaggtactgcgaggacaatgtggatgactgtgcctcttctccctgtgcaaacgggggcacctgccgggacagtgtgaacgatttctcctgtacctgcccacctggctacacaggcaggaactgcagcgcccctgtcagcaggtgtgagcatgcaccctgtcataacggggccacctgccaccagaggggccaacgctacatgtgtgagtgcgcccagggctatggcggcgccaactgccagttcctgctccctgagccaccaccagacctcatagtggcggcccagggcgggtccttcccctgggtggctgtgtgtgccggggtggtgcttgtcctcctgctgctgctgggctgtgctgctgtggtggtctgcgtccggctgaagctacagaaacaccagcctccgcctgatccttgcgggggagagacagagaccatgaacaacctagccaattgccagcgtgagaaggatgtttctgttagcatcattggggctacacagatcaagaacaccaacaagaaggcggactttcatggggaccatggtgctgacaagagcagctttaaggcccgataccccactgtggactataacctcattcgagacctcaagggagatgaagccacggtcagggatgcacacagcaaacgtgacaccaagtgccagtcacagggctctgtaggagaagagaagagcacctcaacgctcaggggtggggaggttcccgacagaaaaaggcccgagtctgtctactctacttcaaaggacaccaagtaccagtcggtgtatgttctatctgcagaaaaggatgagtgtgttatagcgactgaggttgtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccctgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtcttag BBa_K415506_sequence 1 gctccgaattcgcccttcaggtccgaggttctagacgagtttactccctatcagtgatagagaacgatgtcgagtttactccctatcagtgatagagaacgtatgtcgagtttactccctatcagtgatagagaacgtatgtcgagtttactccctatcagtgatagagaacgtatgtcgagtttatccctatcagtgatagagaacgtatgtcgagtttactccctatcagtgatagagaacgtatgtcgaggtaggcgtgtacggtgggaggcctatataagcagagctcgtttagtgaaccgtcagatcgcaaagggcgaattcgac igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z