BBa_K511815 1 BBa_K511815 Inducible Red Fluorescent Protein Generator (TRE-Tight-mKate) MammoBlock Device 2011-09-27T11:00:00Z 2015-05-08T01:12:31Z Consult constituent components. This MammoBlock composite device produces the monomeric red fluorescent protein mKate when induced with tTA/rtTA transactivator variants in the presence of tetracycline analogues, and otherwise produces mKate at a low, basal (OFF) level. false false _674_ 0 6719 9 Not in stock false In order to adhere to the recombination-based MammoBlock standard, and because shipping Biosafety Level 2 mammalian expression vectors to the Registry of Standard Biological Parts is not currently possible, we are providing the sequence resulting from Gateway recombination of the constituent MammoBlock parts here without redistributing the physical DNA plasmids to the Registry. In future years, we hope to make use of improvements in the Registry's accommodations to provide our complete expression vectors. false Grant Robinson component2146194 1 BBa_K415506 component2146196 1 BBa_J85600 component2146198 1 BBa_K511102 annotation2146194 1 BBa_K415506 range2146194 1 1 330 annotation2146196 1 BBa_J85600 range2146196 1 331 352 annotation2146198 1 BBa_K511102 range2146198 1 353 1066 BBa_K415506 1 BBa_K415506 pTRE-Tight L4R1 MammoBlock 2010-10-26T11:00:00Z 2015-05-08T01:12:27Z Source: modified TRE promoter. This inducible promoter can be switched on by the activity of rttA3 transcription factor. It has been optimized to contain multiple TRE binding sites; this makes the 'tight' version a significant improvement over the previously used simple TRE promoter. false true _525_ 0 6768 9 It's complicated false Increased number of TRE operator binding sites. false Laura Deming, Joy Jiao, Adrian Slusarczyk annotation2109714 1 TetO Site5 range2109714 1 188 205 annotation2109711 1 TetO Site2 range2109711 1 81 98 annotation2109712 1 TetO Site3 range2109712 1 117 132 annotation2109715 1 TetO Site6 range2109715 1 224 241 annotation2109713 1 TetO Site4 range2109713 1 153 170 annotation2109710 1 TetO Site1 range2109710 1 46 63 BBa_J85600 1 BBa_J85600 attB1 recombination site 2011-09-05T11:00:00Z 2015-05-08T01:08:30Z lambda phage attB1 recombination site Used for creating mammoblock (RFC65) composite promoter-gene pairs using recombination-based cloning. false false _406_ 0 106 406 Not in stock false None false Ron Weiss annotation2125966 1 attB1 range2125966 1 2 22 BBa_K511102 1 BBa_K511102 mKate MammoBlock 2011-09-24T11:00:00Z 2015-05-08T01:12:31Z This protein was codon-optimized for mammalian expression and is closely homologous to the natural green fluorescent protein derived from Aequorea victoria. A mammalian-codon optimized red fluorescent protein. This protein is a significantly engineered reporter protein that, unlikely many fluorescent proteins, is monomeric and therefore ideal for fusion constructs. Figures 1 and 2 show typical levels of mKate-mediated fluorescence following transfection of HEK-293 cells with mKate driven by the Hef1a low-level constitutive promoter. false false _674_ 0 6719 9 It's complicated false Not applicable. false Grant Robinson annotation2139566 1 mKate Fluorescent Protein range2139566 1 1 714 BBa_J85600_sequence 1 ccaagtttgtacaaaaaagcag BBa_K511815_sequence 1 gctccgaattcgcccttcaggtccgaggttctagacgagtttactccctatcagtgatagagaacgatgtcgagtttactccctatcagtgatagagaacgtatgtcgagtttactccctatcagtgatagagaacgtatgtcgagtttactccctatcagtgatagagaacgtatgtcgagtttatccctatcagtgatagagaacgtatgtcgagtttactccctatcagtgatagagaacgtatgtcgaggtaggcgtgtacggtgggaggcctatataagcagagctcgtttagtgaaccgtcagatcgcaaagggcgaattcgacccaagtttgtacaaaaaagcagatggtgtctaagggcgaagagctgattaaggagaacatgcacatgaagctgtacatggagggcaccgtgaacaaccaccacttcaagtgcacatccgagggcgaaggcaagccctacgagggcacccagaccatgagaatcaaggtggtcgagggcggccctctccccttcgccttcgacatcctggctaccagcttcatgtacggcagcaaaaccttcatcaaccacacccagggcatccccgacttctttaagcagtccttccctgagggcttcacatgggagagagtcaccacatacgaagacgggggcgtgctgaccgctacccaggacaccagcctccaggacggctgcctcatctacaacgtcaagatcagaggggtgaacttcccatccaacggccctgtgatgcagaagaaaacactcggctgggaggcctccaccgagatgctgtaccccgctgacggcggcctggaaggcagaagcgacatggccctgaagctcgtgggcgggggccacctgatctgcaacttgaagaccacatacagatccaagaaacccgctaagaacctcaagatgcccggcgtctactatgtggacagaagactggaaagaatcaaggaggccgacaaagagacctacgtcgagcagcacgaggtggctgtggccagatactgcgacctccctagcaaactggggcacaaacttaattga BBa_K415506_sequence 1 gctccgaattcgcccttcaggtccgaggttctagacgagtttactccctatcagtgatagagaacgatgtcgagtttactccctatcagtgatagagaacgtatgtcgagtttactccctatcagtgatagagaacgtatgtcgagtttactccctatcagtgatagagaacgtatgtcgagtttatccctatcagtgatagagaacgtatgtcgagtttactccctatcagtgatagagaacgtatgtcgaggtaggcgtgtacggtgggaggcctatataagcagagctcgtttagtgaaccgtcagatcgcaaagggcgaattcgac BBa_K511102_sequence 1 atggtgtctaagggcgaagagctgattaaggagaacatgcacatgaagctgtacatggagggcaccgtgaacaaccaccacttcaagtgcacatccgagggcgaaggcaagccctacgagggcacccagaccatgagaatcaaggtggtcgagggcggccctctccccttcgccttcgacatcctggctaccagcttcatgtacggcagcaaaaccttcatcaaccacacccagggcatccccgacttctttaagcagtccttccctgagggcttcacatgggagagagtcaccacatacgaagacgggggcgtgctgaccgctacccaggacaccagcctccaggacggctgcctcatctacaacgtcaagatcagaggggtgaacttcccatccaacggccctgtgatgcagaagaaaacactcggctgggaggcctccaccgagatgctgtaccccgctgacggcggcctggaaggcagaagcgacatggccctgaagctcgtgggcgggggccacctgatctgcaacttgaagaccacatacagatccaagaaacccgctaagaacctcaagatgcccggcgtctactatgtggacagaagactggaaagaatcaaggaggccgacaaagagacctacgtcgagcagcacgaggtggctgtggccagatactgcgacctccctagcaaactggggcacaaacttaattga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z