LOCUS BBa_K511818 6427 bp DNA linear 22-Dec-2024 DEFINITION Inducible Notch-Gal4-ESN Receptor Generator (TRE-Tight-Notch-Gal4-E SN) MammoBlock Device ACCESSION BBa_K511818 VERSION BBa_K511818.1 FEATURES Location/Qualifiers misc_feature 46..63 /label=TetO Site1 misc_feature 81..98 /label=TetO Site2 misc_feature 117..132 /label=TetO Site3 misc_feature 153..170 /label=TetO Site4 misc_feature 188..205 /label=TetO Site5 misc_feature 224..241 /label=TetO Site6 promoter 1..330 /label=BBa_K415506 misc_feature 332..352 /label=attB1 misc_feature 331..352 /label=BBa_J85600 CDS 353..5575 /label=Notch-1 Extracellular Domain misc_feature 5576..5641 /label=Transmembrane Linker CDS 5642..6424 /label=Gal4-ESN Minimal Transactivation Domain misc_feature 353..6427 /label=BBa_K511300 ORIGIN 1 gctccgaatt cgcccttcag gtccgaggtt ctagacgagt ttactcccta tcagtgatag 61 agaacgatgt cgagtttact ccctatcagt gatagagaac gtatgtcgag tttactccct 121 atcagtgata gagaacgtat gtcgagttta ctccctatca gtgatagaga acgtatgtcg 181 agtttatccc tatcagtgat agagaacgta tgtcgagttt actccctatc agtgatagag 241 aacgtatgtc gaggtaggcg tgtacggtgg gaggcctata taagcagagc tcgtttagtg 301 aaccgtcaga tcgcaaaggg cgaattcgac ccaagtttgt acaaaaaagc agatgccgcc 361 gctcctggcg cccctgctct gcctggcgct gctgcccgcg ctcgccgcac gaggcccgcg 421 atgctcccag cccggtgaga cctgcctgaa tggcgggaag tgtgaagcgg ccaatggcac 481 ggaggcctgc gtctgtggcg gggccttcgt gggcccgcga tgccaggacc ccaacccgtg 541 cctcagcacc ccctgcaaga acgccgggac atgccacgtg gtggaccgcg gcagactatg 601 cctgcagctg tgccctgggc ttctctgggc ccctctgcct gacacccctg gacaatgcct 661 gcctcaccaa cccctgccgc aacgggggca cctgcgacct gctcacgctg acggagtaca 721 agtgccgctg cccgcccggc tggtcaggga aatcgtgcca gcaggctgac ccgtgcgcct 781 ccaacccctg cgccaacggt ggccagtgcc tgcccttcga ggcctcctac atctgccact 841 gcccacccag cttccatggc cccacctgcc ggcaggatgt caacgagtgt ggccagaagc 901 ccgggctttg ccgccacgga ggcacctgcc acaacgaggt cggctcctac cgctgcgtct 961 gccgcgccac ccacactggc cccaactgcg agcggcccta cgtgccctgc agcccctcgc 1021 cctgccagaa cgggggcacc tgccgcccca cgggcgacgt cacccacgag tgtgcctgcc 1081 tgccaggctt caccggccag aactgtgagg aaaatatcga cgattgtcca ggaaacaact 1141 gcaagaacgg gggtgcctgt gtggacggcg tgaacaccta caactgccgc tgcccgccag 1201 agtggacagg tcagtactgt accgaggatg tggacgagtg ccagctgatg ccaaatgcct 1261 gccagaacgg cgggacctgc cacaacaccc acggtggcta caactgcgtg tgtgtcaacg 1321 gctggactgg tgaggactgc agcgagaaca ttgatgactg tgccagcgcc gcctgcttcc 1381 acggcgccac ctgccatgac cgtgtggcct ccttctactg cgagtgtccc catggccgca 1441 caggtctgct gtgccacctc aacgacgcat gcatcagcaa cccctgtaac gagggctcca 1501 actgcgacac caaccctgtc aatggcaagg ccatctgcac ctgcccctcg gggtacacgg 1561 gcccggcctg cagccaggac gtggatgagt gctcgctggg tgccaacccc tgcgagcatg 1621 cgggcaagtg catcaacacg ctgggctcct tcgagtgcca gtgtctgcag ggctacacgg 1681 gcccccgatg cgagatcgac gtcaacgagt gcgtctcgaa cccgtgccag aacgacgcca 1741 cctgcctgga ccagattggg gagttccagt gcatctgcat gcccggctac gagggtgtgc 1801 actgcgaggt caacacagac gagtgtgcca gcagcccctg cctgcacaat ggccgctgcc 1861 tggacaagat caatgagttc cagtgcgagt gccccacggg cttcactggg catctgtgcc 1921 agtacgatgt ggacgagtgt gccagcaccc cctgcaagaa tggtgccaag tgcctggacg 1981 gacccaacac ttacacctgt gtgtgcacgg aagggtacac ggggacgcac tgcgaggtgg 2041 acatcgatga gtgcgacccc gacccctgcc actacggctc ctgcaaggac ggcgtcgcca 2101 ccttcacctg cctctgccgc ccaggctaca cgggccacca ctgcgagacc aacatcaacg 2161 agtgctccag ccagccctgc cgccacgggg gcacctgcca ggaccgcgac aacgcctacc 2221 tctgcttctg cctgaagggg accacaggac ccaactgcga gatcaacctg gatgactgtg 2281 ccagcagccc ctgcgactcg ggcacctgtc tggacaagat cgatggctac gagtgtgcct 2341 gtgagccggg ctacacaggg agcatgtgta acatcaacat cgatgagtgt gcgggcaacc 2401 cctgccacaa cgggggcacc tgcgaggacg gcatcaatgg cttcacctgc cgctgccccg 2461 agggctacca cgaccccacc tgcctgtctg aggtcaatga gtgcaacagc aacccctgcg 2521 tccacggggc ctgccgggac agcctcaacg ggtacaagtg cgactgtgac cctgggtgga 2581 gtgggaccaa ctgtgacatc aacaacaatg agtgtgaatc caacccttgt gtcaacggcg 2641 gcacctgcaa agacatgacc agtggctacg tgtgcacctg ccgggagggc ttcagcggtc 2701 ccaactgcca gaccaacatc aacgagtgtg cgtccaaccc atgtctgaac cagggcacgt 2761 gtattgacga cgttgccggg tacaagtgca actgcctgct gccctacaca ggtgccacgt 2821 gtgaggtggt gctggccccg tgtgccccca gcccctgcag aaacggcggg gagtgcaggc 2881 aatccgagga ctatgagagc ttctcctgtg tctgccccac gggctggcaa gcagggcaga 2941 cctgtgaggt cgacatcaac gagtgcgttc tgagcccgtg ccggcacggc gcatcctgcc 3001 agaacaccca cggcggctac cgctgccact gccaggccgg ctacagtggg cgcaactgcg 3061 agaccgacat cgacgactgc cggcccaacc cgtgtcacaa cgggggctcc tgcacagacg 3121 gcatcaacac ggccttctgc gactgcctgc ccggcttccg gggcactttc tgtgaggagg 3181 acatcaacga gtgtgccagt gacccctgcc gcaacggggc caactgcacg gactgcgtgg 3241 acagctacac gtgcacctgc cccgcaggct tcagcgggat ccactgtgag aacaacacgc 3301 ctgactgcac agagagctcc tgcttcaacg gtggcacctg cgtggacggc atcaactcgt 3361 tcacctgcct gtgtccaccc ggcttcacgg gcagctactg ccagcacgat gtcaatgagt 3421 gcgactcaca gccctgcctg catggcggca cctgtcagga cggctgcggc tcctacaggt 3481 gcacctgccc ccagggctac actggcccca actgccagaa ccttgtgcac tggtgtgact 3541 cctcgccctg caagaacggc ggcaaatgct ggcagaccca cacccagtac cgctgcgagt 3601 gccccagcgg ctggaccggc ctttactgcg acgtgcccag cgtgtcctgt gaggtggctg 3661 cgcagcgaca aggtgttgac gttgcccgcc tgtgccagca tggagggctc tgtgtggacg 3721 cgggcaacac gcaccactgc cgctgccagg cgggctacac aggcagctac tgtgaggacc 3781 tggtggacga gtgctcaccc agcccctgcc agaacggggc cacctgcacg gactacctgg 3841 gcggctactc ctgcaagtgc gtggccggct accacggggt gaactgctct gaggagatcg 3901 acgagtgcct ctcccacccc tgccagaacg ggggcacctg cctcgacctc cccaacacct 3961 acaagtgctc ctgcccacgg ggcactcagg gtgtgcactg tgagatcaac gtggacgact 4021 gcaatccccc cgttgacccc gtgtcccgga gccccaagtg ctttaacaac ggcacctgcg 4081 tggaccaggt gggcggctac agctgcacct gcccgccggg cttcgtgggt gagcgctgtg 4141 agggggatgt caacgagtgc ctgtccaatc cctgcgacgc ccgtggcacc cagaactgcg 4201 tgcagcgcgt caatgacttc cactgcgagt gccgtgctgg tcacaccggg cgccgctgcg 4261 agtccgtcat caatggctgc aaaggcaagc cctgcaagaa tgggggcacc tgcgccgtgg 4321 cctccaacac cgcccgcggg ttcatctgca agtgccctgc gggcttcgag ggcgccacgt 4381 gtgagaatga cgctcgtacc tgcggcagcc tgcgctgcct caacggcggc acatgcatct 4441 ccggcccgcg cagccccacc tgcctgtgcc tgggcccctt cacgggcccc gaatgccagt 4501 tcccggccag cagcccctgc ctgggcggca acccctgcta caaccagggg acctgtgagc 4561 ccacatccga gagccccttc taccgttgcc tgtgccccgc caaattcaac gggctcttgt 4621 gccacatcct ggactacagc ttcgggggtg gggccgggcg cgacatcccc ccgccgctga 4681 tcgaggaggc gtgcgagctg cccgagtgcc aggaggacgc gggcaacaag gtctgcagcc 4741 tgcagtgcaa caaccacgcg tgcggctggg acggcggtga ctgctccctc aacttcaatg 4801 acccctggaa gaactgcacg cagtctctgc agtgctggaa gtacttcagt gacggccact 4861 gtgacagcca gtgcaactca gccggctgcc tcttcgacgg ctttgactgc cagcgtgcgg 4921 aaggccagtg caaccccctg tacgaccagt actgcaagga ccacttcagc gacgggcact 4981 gcgaccaggg ctgcaacagc gcggagtgcg agtgggacgg gctggactgt gcggagcatg 5041 tacccgagag gctggcggcc ggcacgctgg tggtggtggt gctgatgccg ccggagcagc 5101 tgcgcaacag ctccttccac ttcctgcggg agctcagccg cgtgctgcac accaacgtgg 5161 tcttcaagcg tgacgcacac ggccagcaga tgatcttccc ctactacggc cgcgaggagg 5221 agctgcgcaa gcaccccatc aagcgtgccg ccgagggctg ggccgcacct gacgccctgc 5281 tgggccaggt gaaggcctcg ctgctccctg gtggcagcga gggtgggcgg cggcggaggg 5341 agctggaccc catggacgtc cgcggctcca tcgtctacct ggagattgac aaccggcagt 5401 gtgtgcaggc ctcctcgcag tgcttccaga gtgccaccga cgtggccgca ttcctgggag 5461 cgctcgcctc gctgggcagc ctcaacatcc cctacaagat cgaggccgtg cagagtgaga 5521 ccgtggagcc gcccccgccg gcgcagctgc acttcatgta cgtggcggcg gccgcgcggc 5581 cgcctttgtg cttctgttct tcgtgggctg cggggtgctg ctgtcccgca agcgccggcg 5641 gatgaagcta ctgtcttcta tcgaacaagc atgcgatatt tgccgactta aaaagctcaa 5701 gtgctccaaa gaaaaaccga agtgcgccaa gtgtctgaag aacaactggg agtgtcgcta 5761 ctctcccaaa accaaaaggt ctccgctgac tagggcacat ctgacagaag tggaatcaag 5821 gctagaaaga ctggaacagc tatttctact gatttttcct cgagaagacc ttgacatgat 5881 tttgaaaatg gattctttac aggatataaa agcattgtta acaggattat ttgtacaaga 5941 taatgtgaat aaagatgccg tcacagatag attggcttca gtggagactg atatgcctct 6001 aacattgaga cagcatagaa taagtgcgac atcatcatcg gaagagagta gtaacaaagg 6061 tcaaagacag ttgactgtat cggccaattt taatcaaagt gggaatattg ctgatagctc 6121 attgtccttc actttcacta acagtagcaa cggtccgaac ctcataacaa ctcaaacaaa 6181 ttctcaagcg ctttcacaac caattgcctc ctctaacgtt catgataact tcatgaataa 6241 tgaaatcacg gctagtaaaa ttgatgatgg taataattca aaaccactgt cacctggttg 6301 gacggaccaa actgcgtata acgcgtttgg aatcactaca gggatgttta ataccactac 6361 aatggatgat gtatataact atctattcga tgatgaagat accccaccaa acccaaaaaa 6421 agagtaa //