BBa_K511821 1 BBa_K511821 Inducible Fluorescence/Repression Generator (UAS-Gal4-amCyan-miRFF4) MammoBlock Device 2011-09-27T11:00:00Z 2015-05-08T01:12:31Z Consult constituent components. This MammoBlock composite device produces the cyan fluorescent protein amCyan-miRFF4 when induced with Gal4 transactivator variants and otherwise produces amCyan-miRFF4 at a low, basal (OFF) level. Note that the presence of the miRFF4 micro RNA tag on amCyan means this MammoBlock device also acts as a repressor. false false _674_ 0 6719 9 Not in stock false In order to adhere to the recombination-based MammoBlock standard, and because shipping Biosafety Level 2 mammalian expression vectors to the Registry of Standard Biological Parts is not currently possible, we are providing the sequence resulting from Gateway recombination of the constituent MammoBlock parts here without redistributing the physical DNA plasmids to the Registry. In future years, we hope to make use of improvements in the Registry's accommodations to provide our complete expression vectors. false Grant Robinson component2146264 1 BBa_K511103 component2146262 1 BBa_J85600 component2146260 1 BBa_K511003 annotation2146262 1 BBa_J85600 range2146262 1 221 242 annotation2146260 1 BBa_K511003 range2146260 1 1 220 annotation2146264 1 BBa_K511103 range2146264 1 243 1308 BBa_K511003 1 BBa_K511003 UAS-Gal4 Promoter MammoBlock 2011-09-24T11:00:00Z 2015-05-08T01:12:31Z This part was synthesized to include the upstream activator sequence (UAS) from the Saccharomyces cerevisiae genome that binds to the Gal4 transactivator upstream of the major-late adenovirus promoter. This part encodes a promoter that is inducible by variants of the Gal4 transactivator and off otherwise. As shown in Figures 1 and 2, fluorescence from a red fluorescent protein (mKate) driven by the UAS-Gal4 promoter is very strong in the presence of constitutively produced Gal4 derivative with an attached viral VP16 transactivation domain (Gal4-VP16), and is essentially off in the absences of the Gal4-VP16 transactivator. false false _674_ 0 6719 9 It's complicated true Not applicable. false Grant Robinson annotation2139552 1 Gal4-Binding Region range2139552 1 46 148 annotation2139553 1 Major-Late Adenovirus Promoter range2139553 1 160 200 BBa_J85600 1 BBa_J85600 attB1 recombination site 2011-09-05T11:00:00Z 2015-05-08T01:08:30Z lambda phage attB1 recombination site Used for creating mammoblock (RFC65) composite promoter-gene pairs using recombination-based cloning. false false _406_ 0 106 406 Not in stock false None false Ron Weiss annotation2125966 1 attB1 range2125966 1 2 22 BBa_K511103 1 BBa_K511103 amCyan-miRFF4 MammoBlock 2011-09-27T11:00:00Z 2015-05-08T01:12:31Z This protein is an engineered version of the original green fluorescent protein from Aequorea victoria that has ben modified to fluoresce cyan and includes a concatenation of the synthetic FF4 miRNA sequence. This part is an engineered variant of the original green fluorescent protein from Aequorea victoria that fluoresces cyan. Additionally, this protein contains an intronic micro RNA (miRNA) that binds tightly to the FF4 sequence. This protein is therefore a dual repressor/reporter that typically serves to indicate which cells have miRFF4 in mammalian experiments. false false _674_ 0 6719 9 It's complicated false Not applicable. false Grant Robinson annotation2145551 1 amCyan-miRFF4 range2145551 1 1 1063 BBa_J85600_sequence 1 ccaagtttgtacaaaaaagcag BBa_K511103_sequence 1 atggccctgtccaacaagttcatcggcgacgacatgaagatgacctaccacatggacggctgcgtgaacggccactacttcaccgtgaagggcgaggtgagtatgtgctcgcttcggcagcacatatactatgttgaatgaggcttcagtactttacagaatcgttgcctgcacatcttggaaacacttgctgggattacttcttcaggttaacccaacagaaggctcgagtgctgttgacagtgagcgccgcttgaagtttttaattaaatagtgaagccacagatgtatttaattaaagacttcaagcggtgcctactgcctcggagaattcaaggggctactttaggagcaattatcttgtttactaaaactgaataccttgctatctctttgatacatttttacaaagctgaattaaaatggtataaattaaatcacttttttcaattgtttccttttttttcctcagggcagcggcaagccctacgagggcacccagacctccaccttcaaggtgaccatggccaacggcggccccctggccttctccttcgacatcctgtccaccgtgttcatgtacggcaaccgctgcttcaccgcctaccccaccagcatgcccgactacttcaagcaggccttccccgacggcatgtcctacgagagaaccttcacctacgaggacggcggcgtggccaccgccagctgggagatcagcctgaagggcaactgcttcgagcacaagtccaccttccacggcgtgaacttccccgccgacggccccgtgatggccaagaagaccaccggctgggacccctccttcgagaagatgaccgtgtgcgacggcatcttgaagggcgacgtgaccgccttcctgatgctgcagggcggcggcaactacagatgccagttccacacctcctacaagaccaagaagcccgtgaccatgccccccaaccacgtggtggagcaccgcatcgccagaaccgacctggacaagggcggcaacagcgtgcagctgaccgagcacgccgtggcccacatcacctccgtggtgcccttctaa BBa_K511821_sequence 1 gctccgaattgggacagcagagatccagtttggttaattaatagacggagtactgtcctccgagcggagtactgtcctccgactcgagcggagtactgtcctccgatcggagtactgtcctccgcgaattccggagtactgtcctccgaagacgctagcggggggctataaaagggggtgggggcgttcgtcctcactctcaattcggcccaattcgaccccaagtttgtacaaaaaagcagatggccctgtccaacaagttcatcggcgacgacatgaagatgacctaccacatggacggctgcgtgaacggccactacttcaccgtgaagggcgaggtgagtatgtgctcgcttcggcagcacatatactatgttgaatgaggcttcagtactttacagaatcgttgcctgcacatcttggaaacacttgctgggattacttcttcaggttaacccaacagaaggctcgagtgctgttgacagtgagcgccgcttgaagtttttaattaaatagtgaagccacagatgtatttaattaaagacttcaagcggtgcctactgcctcggagaattcaaggggctactttaggagcaattatcttgtttactaaaactgaataccttgctatctctttgatacatttttacaaagctgaattaaaatggtataaattaaatcacttttttcaattgtttccttttttttcctcagggcagcggcaagccctacgagggcacccagacctccaccttcaaggtgaccatggccaacggcggccccctggccttctccttcgacatcctgtccaccgtgttcatgtacggcaaccgctgcttcaccgcctaccccaccagcatgcccgactacttcaagcaggccttccccgacggcatgtcctacgagagaaccttcacctacgaggacggcggcgtggccaccgccagctgggagatcagcctgaagggcaactgcttcgagcacaagtccaccttccacggcgtgaacttccccgccgacggccccgtgatggccaagaagaccaccggctgggacccctccttcgagaagatgaccgtgtgcgacggcatcttgaagggcgacgtgaccgccttcctgatgctgcagggcggcggcaactacagatgccagttccacacctcctacaagaccaagaagcccgtgaccatgccccccaaccacgtggtggagcaccgcatcgccagaaccgacctggacaagggcggcaacagcgtgcagctgaccgagcacgccgtggcccacatcacctccgtggtgcccttctaa BBa_K511003_sequence 1 gctccgaattgggacagcagagatccagtttggttaattaatagacggagtactgtcctccgagcggagtactgtcctccgactcgagcggagtactgtcctccgatcggagtactgtcctccgcgaattccggagtactgtcctccgaagacgctagcggggggctataaaagggggtgggggcgttcgtcctcactctcaattcggcccaattcgacc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z