LOCUS BBa_K511825 6317 bp DNA linear 29-Nov-2024 DEFINITION Inducible Notch-Gal4-ESN Receptor Generator (UAS-Gal4-Notch-Gal4-ES N) MammoBlock Device ACCESSION BBa_K511825 VERSION BBa_K511825.1 FEATURES Location/Qualifiers misc_feature 46..148 /label=Gal4-Binding Region promoter 160..200 /label=Major-Late Adenovirus Promoter promoter 1..220 /label=BBa_K511003 misc_feature 222..242 /label=attB1 misc_feature 221..242 /label=BBa_J85600 CDS 243..5465 /label=Notch-1 Extracellular Domain misc_feature 5466..5531 /label=Transmembrane Linker CDS 5532..6314 /label=Gal4-ESN Minimal Transactivation Domain misc_feature 243..6317 /label=BBa_K511300 ORIGIN 1 gctccgaatt gggacagcag agatccagtt tggttaatta atagacggag tactgtcctc 61 cgagcggagt actgtcctcc gactcgagcg gagtactgtc ctccgatcgg agtactgtcc 121 tccgcgaatt ccggagtact gtcctccgaa gacgctagcg gggggctata aaagggggtg 181 ggggcgttcg tcctcactct caattcggcc caattcgacc ccaagtttgt acaaaaaagc 241 agatgccgcc gctcctggcg cccctgctct gcctggcgct gctgcccgcg ctcgccgcac 301 gaggcccgcg atgctcccag cccggtgaga cctgcctgaa tggcgggaag tgtgaagcgg 361 ccaatggcac ggaggcctgc gtctgtggcg gggccttcgt gggcccgcga tgccaggacc 421 ccaacccgtg cctcagcacc ccctgcaaga acgccgggac atgccacgtg gtggaccgcg 481 gcagactatg cctgcagctg tgccctgggc ttctctgggc ccctctgcct gacacccctg 541 gacaatgcct gcctcaccaa cccctgccgc aacgggggca cctgcgacct gctcacgctg 601 acggagtaca agtgccgctg cccgcccggc tggtcaggga aatcgtgcca gcaggctgac 661 ccgtgcgcct ccaacccctg cgccaacggt ggccagtgcc tgcccttcga ggcctcctac 721 atctgccact gcccacccag cttccatggc cccacctgcc ggcaggatgt caacgagtgt 781 ggccagaagc ccgggctttg ccgccacgga ggcacctgcc acaacgaggt cggctcctac 841 cgctgcgtct gccgcgccac ccacactggc cccaactgcg agcggcccta cgtgccctgc 901 agcccctcgc cctgccagaa cgggggcacc tgccgcccca cgggcgacgt cacccacgag 961 tgtgcctgcc tgccaggctt caccggccag aactgtgagg aaaatatcga cgattgtcca 1021 ggaaacaact gcaagaacgg gggtgcctgt gtggacggcg tgaacaccta caactgccgc 1081 tgcccgccag agtggacagg tcagtactgt accgaggatg tggacgagtg ccagctgatg 1141 ccaaatgcct gccagaacgg cgggacctgc cacaacaccc acggtggcta caactgcgtg 1201 tgtgtcaacg gctggactgg tgaggactgc agcgagaaca ttgatgactg tgccagcgcc 1261 gcctgcttcc acggcgccac ctgccatgac cgtgtggcct ccttctactg cgagtgtccc 1321 catggccgca caggtctgct gtgccacctc aacgacgcat gcatcagcaa cccctgtaac 1381 gagggctcca actgcgacac caaccctgtc aatggcaagg ccatctgcac ctgcccctcg 1441 gggtacacgg gcccggcctg cagccaggac gtggatgagt gctcgctggg tgccaacccc 1501 tgcgagcatg cgggcaagtg catcaacacg ctgggctcct tcgagtgcca gtgtctgcag 1561 ggctacacgg gcccccgatg cgagatcgac gtcaacgagt gcgtctcgaa cccgtgccag 1621 aacgacgcca cctgcctgga ccagattggg gagttccagt gcatctgcat gcccggctac 1681 gagggtgtgc actgcgaggt caacacagac gagtgtgcca gcagcccctg cctgcacaat 1741 ggccgctgcc tggacaagat caatgagttc cagtgcgagt gccccacggg cttcactggg 1801 catctgtgcc agtacgatgt ggacgagtgt gccagcaccc cctgcaagaa tggtgccaag 1861 tgcctggacg gacccaacac ttacacctgt gtgtgcacgg aagggtacac ggggacgcac 1921 tgcgaggtgg acatcgatga gtgcgacccc gacccctgcc actacggctc ctgcaaggac 1981 ggcgtcgcca ccttcacctg cctctgccgc ccaggctaca cgggccacca ctgcgagacc 2041 aacatcaacg agtgctccag ccagccctgc cgccacgggg gcacctgcca ggaccgcgac 2101 aacgcctacc tctgcttctg cctgaagggg accacaggac ccaactgcga gatcaacctg 2161 gatgactgtg ccagcagccc ctgcgactcg ggcacctgtc tggacaagat cgatggctac 2221 gagtgtgcct gtgagccggg ctacacaggg agcatgtgta acatcaacat cgatgagtgt 2281 gcgggcaacc cctgccacaa cgggggcacc tgcgaggacg gcatcaatgg cttcacctgc 2341 cgctgccccg agggctacca cgaccccacc tgcctgtctg aggtcaatga gtgcaacagc 2401 aacccctgcg tccacggggc ctgccgggac agcctcaacg ggtacaagtg cgactgtgac 2461 cctgggtgga gtgggaccaa ctgtgacatc aacaacaatg agtgtgaatc caacccttgt 2521 gtcaacggcg gcacctgcaa agacatgacc agtggctacg tgtgcacctg ccgggagggc 2581 ttcagcggtc ccaactgcca gaccaacatc aacgagtgtg cgtccaaccc atgtctgaac 2641 cagggcacgt gtattgacga cgttgccggg tacaagtgca actgcctgct gccctacaca 2701 ggtgccacgt gtgaggtggt gctggccccg tgtgccccca gcccctgcag aaacggcggg 2761 gagtgcaggc aatccgagga ctatgagagc ttctcctgtg tctgccccac gggctggcaa 2821 gcagggcaga cctgtgaggt cgacatcaac gagtgcgttc tgagcccgtg ccggcacggc 2881 gcatcctgcc agaacaccca cggcggctac cgctgccact gccaggccgg ctacagtggg 2941 cgcaactgcg agaccgacat cgacgactgc cggcccaacc cgtgtcacaa cgggggctcc 3001 tgcacagacg gcatcaacac ggccttctgc gactgcctgc ccggcttccg gggcactttc 3061 tgtgaggagg acatcaacga gtgtgccagt gacccctgcc gcaacggggc caactgcacg 3121 gactgcgtgg acagctacac gtgcacctgc cccgcaggct tcagcgggat ccactgtgag 3181 aacaacacgc ctgactgcac agagagctcc tgcttcaacg gtggcacctg cgtggacggc 3241 atcaactcgt tcacctgcct gtgtccaccc ggcttcacgg gcagctactg ccagcacgat 3301 gtcaatgagt gcgactcaca gccctgcctg catggcggca cctgtcagga cggctgcggc 3361 tcctacaggt gcacctgccc ccagggctac actggcccca actgccagaa ccttgtgcac 3421 tggtgtgact cctcgccctg caagaacggc ggcaaatgct ggcagaccca cacccagtac 3481 cgctgcgagt gccccagcgg ctggaccggc ctttactgcg acgtgcccag cgtgtcctgt 3541 gaggtggctg cgcagcgaca aggtgttgac gttgcccgcc tgtgccagca tggagggctc 3601 tgtgtggacg cgggcaacac gcaccactgc cgctgccagg cgggctacac aggcagctac 3661 tgtgaggacc tggtggacga gtgctcaccc agcccctgcc agaacggggc cacctgcacg 3721 gactacctgg gcggctactc ctgcaagtgc gtggccggct accacggggt gaactgctct 3781 gaggagatcg acgagtgcct ctcccacccc tgccagaacg ggggcacctg cctcgacctc 3841 cccaacacct acaagtgctc ctgcccacgg ggcactcagg gtgtgcactg tgagatcaac 3901 gtggacgact gcaatccccc cgttgacccc gtgtcccgga gccccaagtg ctttaacaac 3961 ggcacctgcg tggaccaggt gggcggctac agctgcacct gcccgccggg cttcgtgggt 4021 gagcgctgtg agggggatgt caacgagtgc ctgtccaatc cctgcgacgc ccgtggcacc 4081 cagaactgcg tgcagcgcgt caatgacttc cactgcgagt gccgtgctgg tcacaccggg 4141 cgccgctgcg agtccgtcat caatggctgc aaaggcaagc cctgcaagaa tgggggcacc 4201 tgcgccgtgg cctccaacac cgcccgcggg ttcatctgca agtgccctgc gggcttcgag 4261 ggcgccacgt gtgagaatga cgctcgtacc tgcggcagcc tgcgctgcct caacggcggc 4321 acatgcatct ccggcccgcg cagccccacc tgcctgtgcc tgggcccctt cacgggcccc 4381 gaatgccagt tcccggccag cagcccctgc ctgggcggca acccctgcta caaccagggg 4441 acctgtgagc ccacatccga gagccccttc taccgttgcc tgtgccccgc caaattcaac 4501 gggctcttgt gccacatcct ggactacagc ttcgggggtg gggccgggcg cgacatcccc 4561 ccgccgctga tcgaggaggc gtgcgagctg cccgagtgcc aggaggacgc gggcaacaag 4621 gtctgcagcc tgcagtgcaa caaccacgcg tgcggctggg acggcggtga ctgctccctc 4681 aacttcaatg acccctggaa gaactgcacg cagtctctgc agtgctggaa gtacttcagt 4741 gacggccact gtgacagcca gtgcaactca gccggctgcc tcttcgacgg ctttgactgc 4801 cagcgtgcgg aaggccagtg caaccccctg tacgaccagt actgcaagga ccacttcagc 4861 gacgggcact gcgaccaggg ctgcaacagc gcggagtgcg agtgggacgg gctggactgt 4921 gcggagcatg tacccgagag gctggcggcc ggcacgctgg tggtggtggt gctgatgccg 4981 ccggagcagc tgcgcaacag ctccttccac ttcctgcggg agctcagccg cgtgctgcac 5041 accaacgtgg tcttcaagcg tgacgcacac ggccagcaga tgatcttccc ctactacggc 5101 cgcgaggagg agctgcgcaa gcaccccatc aagcgtgccg ccgagggctg ggccgcacct 5161 gacgccctgc tgggccaggt gaaggcctcg ctgctccctg gtggcagcga gggtgggcgg 5221 cggcggaggg agctggaccc catggacgtc cgcggctcca tcgtctacct ggagattgac 5281 aaccggcagt gtgtgcaggc ctcctcgcag tgcttccaga gtgccaccga cgtggccgca 5341 ttcctgggag cgctcgcctc gctgggcagc ctcaacatcc cctacaagat cgaggccgtg 5401 cagagtgaga ccgtggagcc gcccccgccg gcgcagctgc acttcatgta cgtggcggcg 5461 gccgcgcggc cgcctttgtg cttctgttct tcgtgggctg cggggtgctg ctgtcccgca 5521 agcgccggcg gatgaagcta ctgtcttcta tcgaacaagc atgcgatatt tgccgactta 5581 aaaagctcaa gtgctccaaa gaaaaaccga agtgcgccaa gtgtctgaag aacaactggg 5641 agtgtcgcta ctctcccaaa accaaaaggt ctccgctgac tagggcacat ctgacagaag 5701 tggaatcaag gctagaaaga ctggaacagc tatttctact gatttttcct cgagaagacc 5761 ttgacatgat tttgaaaatg gattctttac aggatataaa agcattgtta acaggattat 5821 ttgtacaaga taatgtgaat aaagatgccg tcacagatag attggcttca gtggagactg 5881 atatgcctct aacattgaga cagcatagaa taagtgcgac atcatcatcg gaagagagta 5941 gtaacaaagg tcaaagacag ttgactgtat cggccaattt taatcaaagt gggaatattg 6001 ctgatagctc attgtccttc actttcacta acagtagcaa cggtccgaac ctcataacaa 6061 ctcaaacaaa ttctcaagcg ctttcacaac caattgcctc ctctaacgtt catgataact 6121 tcatgaataa tgaaatcacg gctagtaaaa ttgatgatgg taataattca aaaccactgt 6181 cacctggttg gacggaccaa actgcgtata acgcgtttgg aatcactaca gggatgttta 6241 ataccactac aatggatgat gtatataact atctattcga tgatgaagat accccaccaa 6301 acccaaaaaa agagtaa //