LOCUS       BBa_K511901             2372 bp    DNA     linear       29-Nov-2024
DEFINITION  LacI Repressor Generator (Hef1a-LacI) MammoBlock Device
ACCESSION   BBa_K511901
VERSION     BBa_K511901.1  
FEATURES             Location/Qualifiers
     promoter        1..1243
                     /label=BBa_K415508
     misc_feature    1245..1265
                     /label=attB1
     misc_feature    1244..1265
                     /label=BBa_J85600
     CDS             1266..2366
                     /label=LacI Repressor
     misc_feature    2345..2366
                     /label=Nuclear Localization Sequence
     CDS             1266..2372
                     /label=BBa_K511204
ORIGIN
        1 gctccgaatt cgcccttgac agcagagatc cagtttggtt aattaattgg ctccggtgcc
       61 cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttggtggg aggggtcggc
      121 aattgaaccg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac
      181 tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg
      241 aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt gtgtggttcc
      301 cgcgggcctg gcctctttac gggttatggc ccttgcgtgc cttgaattac ttccacctgg
      361 ctgcagtacg tgattcttga tcccgagctt cgggttggaa gtgggtggga gagttcgagg
      421 ccttgcgctt aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg
      481 ggccgccgcg tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct
      541 ctagccattt aaaatttttg atgacctgct gcgacgcttt ttttctggca agatagtctt
      601 gtaaatgcgg gccaagatct gcacactggt atttcggttt ttggggccgc gggcggcgac
      661 ggggcccgtg cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg
      721 agaatcggac gggggtagtc tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg
      781 ccgtgtatcg ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg
      841 gaaagatggc cgcttcccgg ccctgctgca gggagctcaa aatggaggac gcggcgctcg
      901 ggagagcggg cgggtgagtc acccacacaa aggaaaaggg cctttccgtc ctcagccgtc
      961 gcttcatgtg actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctcgagc
     1021 ttttggagta cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac
     1081 actgagtggg tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa
     1141 tttgcccttt ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt
     1201 ttttttcttc catttcaggt gtcgtgagaa gggcgaattc gacccaagtt tgtacaaaaa
     1261 agcagatgaa accagtaacg ttatacgatg tcgcagagta tgccggtgtc tcttatcaga
     1321 ccgtttcccg cgtggtgaac caggccagcc acgtttctgc gaaaacgcgg gaaaaagtgg
     1381 aagcggcgat ggcggagctg aattacattc ccaaccgcgt ggcacaacaa ctggcgggca
     1441 aacagtcgtt gctgattggc gttgccacct ccagtctggc cctgcacgcg ccgtcgcaaa
     1501 ttgtcgcggc gattaaatct cgcgccgatc aactgggtgc cagcgtggtg gtgtcgatgg
     1561 tagaacgaag cggcgtcgaa gcctgtaaag cggcggtgca caatcttctc gcgcaacgcg
     1621 tcagtgggct gatcattaac tatccgctgg atgaccagga tgccattgct gtggaagctg
     1681 cctgcactaa tgttccggcg ttatttcttg atgtctctga ccagacaccc atcaacagta
     1741 ttattttctc ccatgaagac ggtacgcgac tgggcgtgga gcatctggtc gcattgggtc
     1801 accagcaaat cgcgctgtta gcgggcccat taagttctgt ctcggcgcgt ctgcgtctgg
     1861 ctggctggca taaatatctc actcgcaatc aaattcagcc gatagcggaa cgggaaggcg
     1921 actggagtgc catgtccggt tttcaacaaa ccatgcaaat gctgaatgag ggcatcgttc
     1981 ccactgcgat gctggttgcc aacgatcaga tggcgctggg cgcaatgcgc gccattaccg
     2041 agtccgggct gcgcgttggt gcggatatct cggtagtggg atacgacgat accgaagaca
     2101 gctcatgtta tatcccgccg ttaaccacca tcaaacagga ttttcgcctg ctggggcaaa
     2161 ccagcgtgga ccgcttgctg caactctctc agggccaggc ggtgaagggc aatcagctgt
     2221 tgcccgtctc actggtgaaa agaaaaacca ccctggcgcc caatacgcaa accgcctctc
     2281 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     2341 ggcagccaaa aaagaagaga aaggtctaat ag
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