BBa_K511903 1 BBa_K511903 rtTA-DD Transactivator Generator (Hef1a-rtTA-DD) MammoBlock Device 2011-09-27T11:00:00Z 2015-05-08T01:12:31Z Consult constituent components. This MammoBlock composite device produces the rtTA-DD tetracycline-analogue-inducible and SHIELD-tunable transactivator at a low, constitutive level. false false _674_ 0 6719 9 Not in stock false In order to adhere to the recombination-based MammoBlock standard, and because shipping Biosafety Level 2 mammalian expression vectors to the Registry of Standard Biological Parts is not currently possible, we are providing the sequence resulting from Gateway recombination of the constituent MammoBlock parts here without redistributing the physical DNA plasmids to the Registry. In future years, we hope to make use of improvements in the Registry's accommodations to provide our complete expression vectors. false Grant Robinson component2146345 1 BBa_K511202 component2146342 1 BBa_J85600 component2146340 1 BBa_K415508 annotation2146340 1 BBa_K415508 range2146340 1 1 1243 annotation2146342 1 BBa_J85600 range2146342 1 1244 1265 annotation2146345 1 BBa_K511202 range2146345 1 1266 2600 BBa_J85600 1 BBa_J85600 attB1 recombination site 2011-09-05T11:00:00Z 2015-05-08T01:08:30Z lambda phage attB1 recombination site Used for creating mammoblock (RFC65) composite promoter-gene pairs using recombination-based cloning. false false _406_ 0 106 406 Not in stock false None false Ron Weiss annotation2125966 1 attB1 range2125966 1 2 22 BBa_K511202 1 BBa_K511202 rtTA-DD Transactivator MammoBlock 2011-09-24T11:00:00Z 2015-05-08T01:12:31Z This part consists of an Escherichia coli-derived binding domain for the tetracycline operator, the viral VP16 transactivation domain isolated from the herpes simplex virus, and an engineered human FKBP12 protein that functions as a destabilization domain. This part encodes a protein that combines the rtTA transactivator with the DD destabilization domain. The DD domain causes attached proteins to be degraded extremely quickly in mammalian cells; however, the DD domain is stable in the presence of the SHIELD molecule, allowing for tunable protein expression. Because the rtTA transactivator is dependent upon doxycycline for activation, transactivation by rtTA-DD is unique among transactivators for the dynamic range of expression it can activate. false false _674_ 0 6719 9 It's complicated false Note that the DD domain is positioned before the rtTA transactivator in this part. false Grant Robinson annotation2139589 1 rtTA Transactivation Domain range2139589 1 325 1329 annotation2139588 1 Destabilization Domain range2139588 1 1 324 BBa_K415508 1 BBa_K415508 phEF1a L4R1 MammoBlock Entry Vector 2010-10-26T11:00:00Z 2015-05-08T01:12:27Z MIT iGEM 2010 Mammalian Part MIT iGEM 2010 Mammalian Part false false _525_ 0 7331 9 It's complicated false MIT iGEM 2010 Mammalian Part false Laura Deming, Joy Jiao, Adrian Slusarczyk BBa_J85600_sequence 1 ccaagtttgtacaaaaaagcag BBa_K511903_sequence 1 gctccgaattcgcccttgacagcagagatccagtttggttaattaattggctccggtgcccgtcagtgggcagagcgcacatcgcccacagtccccgagaagttggtgggaggggtcggcaattgaaccggtgcctagagaaggtggcgcggggtaaactgggaaagtgatgtcgtgtactggctccgcctttttcccgagggtgggggagaaccgtatataagtgcagtagtcgccgtgaacgttctttttcgcaacgggtttgccgccagaacacaggtaagtgccgtgtgtggttcccgcgggcctggcctctttacgggttatggcccttgcgtgccttgaattacttccacctggctgcagtacgtgattcttgatcccgagcttcgggttggaagtgggtgggagagttcgaggccttgcgcttaaggagccccttcgcctcgtgcttgagttgaggcctggcctgggcgctggggccgccgcgtgcgaatctggtggcaccttcgcgcctgtctcgctgctttcgataagtctctagccatttaaaatttttgatgacctgctgcgacgctttttttctggcaagatagtcttgtaaatgcgggccaagatctgcacactggtatttcggtttttggggccgcgggcggcgacggggcccgtgcgtcccagcgcacatgttcggcgaggcggggcctgcgagcgcggccaccgagaatcggacgggggtagtctcaagctggccggcctgctctggtgcctggcctcgcgccgccgtgtatcgccccgccctgggcggcaaggctggcccggtcggcaccagttgcgtgagcggaaagatggccgcttcccggccctgctgcagggagctcaaaatggaggacgcggcgctcgggagagcgggcgggtgagtcacccacacaaaggaaaagggcctttccgtcctcagccgtcgcttcatgtgactccacggagtaccgggcgccgtccaggcacctcgattagttctcgagcttttggagtacgtcgtctttaggttggggggaggggttttatgcgatggagtttccccacactgagtgggtggagactgaagttaggccagcttggcacttgatgtaattctccttggaatttgccctttttgagtttggatcttggttcattctcaagcctcagacagtggttcaaagtttttttcttccatttcaggtgtcgtgagaagggcgaattcgacccaagtttgtacaaaaaagcagatgggcgtgcaggtggagaccatcagcccaggagatggcaggacattccccaagcgcgggcagacttgcgtggtgcactacaccggcatgctggaggacgggaagaaagtggatagctccagggaccgcaacaagcccttcaagttcatgctgggaaagcaggaagtgatccgcgggtgggaggaaggagtggcccagatgagtgtgggccagcgggctaaactgactatttcacctgactacgcctatggagctaccggccacccagggatcattccccctcatgccaccctggtgttcgatgtggaactgctgaagccagagatgtcaagactggacaagagcaaagtgatcaactccgccctggagctgctgaatgaagtgggaattgagggcctgaccacaaggaagctggctcagaaactgggcgtggagcagccaactctgtattggcacgtgaagaacaaacgcgccctgctggatgccctggctattgagatgctggaccggcaccatacacatttttgccccctgaagggcgaaagctggcaggatttcctgcggaacaatgccaaatcttttagatgtgctctgctgagtcacaggaatggggccaaggtgcatagcgacacacgccccactgagaaacagtacgagaccctggaaaaccagctggccttcctgtgccagcagggcttttccctggaaaatgccctgtatgctctgtctgccgtgggacacttcacactgggctgcgtgctggaggatcaggaacatcaggtggccaaggaggaaagagagactcccactaccgattccatgccacccctgctgaggcaggccatcgaactgtttgaccaccagggcgctgagcctgccttcctgtttggcctggaactgatcatttgcgggctggaaaagcagctgaaatgcgagtctggcagtgcttacagccgggccagaacaaaaaacaattatgggtccactattgagggactgctggacctgccagacgatgacgctccagaggaagctggactggctgctccaaggctgtctttcctgcctgccggacacacccggagactgtcaaccgctcctccaacagatgtgagcctgggagacgaactgcacctggatggcgaggacgtggccatggctcatgccgatgctctggatgacttcgatctggacatgctgggggatggagactctccaggacctgggtttacccctcatgacagtgctccatacggggccctggatatggctgacttcgagtttgaacagatgtttacagatgccctgggaatcgacgagtatggcggctgatga BBa_K415508_sequence 1 gctccgaattcgcccttgacagcagagatccagtttggttaattaattggctccggtgcccgtcagtgggcagagcgcacatcgcccacagtccccgagaagttggtgggaggggtcggcaattgaaccggtgcctagagaaggtggcgcggggtaaactgggaaagtgatgtcgtgtactggctccgcctttttcccgagggtgggggagaaccgtatataagtgcagtagtcgccgtgaacgttctttttcgcaacgggtttgccgccagaacacaggtaagtgccgtgtgtggttcccgcgggcctggcctctttacgggttatggcccttgcgtgccttgaattacttccacctggctgcagtacgtgattcttgatcccgagcttcgggttggaagtgggtgggagagttcgaggccttgcgcttaaggagccccttcgcctcgtgcttgagttgaggcctggcctgggcgctggggccgccgcgtgcgaatctggtggcaccttcgcgcctgtctcgctgctttcgataagtctctagccatttaaaatttttgatgacctgctgcgacgctttttttctggcaagatagtcttgtaaatgcgggccaagatctgcacactggtatttcggtttttggggccgcgggcggcgacggggcccgtgcgtcccagcgcacatgttcggcgaggcggggcctgcgagcgcggccaccgagaatcggacgggggtagtctcaagctggccggcctgctctggtgcctggcctcgcgccgccgtgtatcgccccgccctgggcggcaaggctggcccggtcggcaccagttgcgtgagcggaaagatggccgcttcccggccctgctgcagggagctcaaaatggaggacgcggcgctcgggagagcgggcgggtgagtcacccacacaaaggaaaagggcctttccgtcctcagccgtcgcttcatgtgactccacggagtaccgggcgccgtccaggcacctcgattagttctcgagcttttggagtacgtcgtctttaggttggggggaggggttttatgcgatggagtttccccacactgagtgggtggagactgaagttaggccagcttggcacttgatgtaattctccttggaatttgccctttttgagtttggatcttggttcattctcaagcctcagacagtggttcaaagtttttttcttccatttcaggtgtcgtgagaagggcgaattcgac BBa_K511202_sequence 1 atgggcgtgcaggtggagaccatcagcccaggagatggcaggacattccccaagcgcgggcagacttgcgtggtgcactacaccggcatgctggaggacgggaagaaagtggatagctccagggaccgcaacaagcccttcaagttcatgctgggaaagcaggaagtgatccgcgggtgggaggaaggagtggcccagatgagtgtgggccagcgggctaaactgactatttcacctgactacgcctatggagctaccggccacccagggatcattccccctcatgccaccctggtgttcgatgtggaactgctgaagccagagatgtcaagactggacaagagcaaagtgatcaactccgccctggagctgctgaatgaagtgggaattgagggcctgaccacaaggaagctggctcagaaactgggcgtggagcagccaactctgtattggcacgtgaagaacaaacgcgccctgctggatgccctggctattgagatgctggaccggcaccatacacatttttgccccctgaagggcgaaagctggcaggatttcctgcggaacaatgccaaatcttttagatgtgctctgctgagtcacaggaatggggccaaggtgcatagcgacacacgccccactgagaaacagtacgagaccctggaaaaccagctggccttcctgtgccagcagggcttttccctggaaaatgccctgtatgctctgtctgccgtgggacacttcacactgggctgcgtgctggaggatcaggaacatcaggtggccaaggaggaaagagagactcccactaccgattccatgccacccctgctgaggcaggccatcgaactgtttgaccaccagggcgctgagcctgccttcctgtttggcctggaactgatcatttgcgggctggaaaagcagctgaaatgcgagtctggcagtgcttacagccgggccagaacaaaaaacaattatgggtccactattgagggactgctggacctgccagacgatgacgctccagaggaagctggactggctgctccaaggctgtctttcctgcctgccggacacacccggagactgtcaaccgctcctccaacagatgtgagcctgggagacgaactgcacctggatggcgaggacgtggccatggctcatgccgatgctctggatgacttcgatctggacatgctgggggatggagactctccaggacctgggtttacccctcatgacagtgctccatacggggccctggatatggctgacttcgagtttgaacagatgtttacagatgccctgggaatcgacgagtatggcggctgatga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z