LOCUS       BBa_K511904             2404 bp    DNA     linear       25-Nov-2024
DEFINITION  Repressible LacI Repressor Generator (Hef1a-LacO-LacI) MammoBlock 
            Device
ACCESSION   BBa_K511904
VERSION     BBa_K511904.1  
FEATURES             Location/Qualifiers
     promoter        1..1275
                     /label=Hef1-alpha Promoter
     misc_feature    201..221
                     /label=Lac O1
     misc_feature    272..291
                     /label=Lac Oid
     promoter        1..1275
                     /label=BBa_K511000
     misc_feature    1277..1297
                     /label=attB1
     misc_feature    1276..1297
                     /label=BBa_J85600
     CDS             1298..2398
                     /label=LacI Repressor
     misc_feature    2377..2398
                     /label=Nuclear Localization Sequence
     CDS             1298..2404
                     /label=BBa_K511204
ORIGIN
        1 tggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg
       61 gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag
      121 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc
      181 agtagtcgcc gtgaagctag aattgtgagc ggataacaat tccacagtcg accctaggtt
      241 gtgtcgcgag tgttggatcc cagctgacac caattgtgag cgctcacaat tgctagccgt
      301 tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg
      361 gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca cctggctgca
      421 gtacgtgatt cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg
      481 cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg
      541 ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc
      601 catttaaaat ttttgatgac ctgctgcgac gctttttttc tggcaagata gtcttgtaaa
      661 tgcgggccaa gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc
      721 ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat
      781 cggacggggg tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg
      841 tatcgccccg ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag
      901 atggccgctt cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga
      961 gcgggcgggt gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc
     1021 atgtgactcc acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg
     1081 gagtacgtcg tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga
     1141 gtgggtggag actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc
     1201 cctttttgag tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt
     1261 tcttccattt caggtccaag tttgtacaaa aaagcagatg aaaccagtaa cgttatacga
     1321 tgtcgcagag tatgccggtg tctcttatca gaccgtttcc cgcgtggtga accaggccag
     1381 ccacgtttct gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc tgaattacat
     1441 tcccaaccgc gtggcacaac aactggcggg caaacagtcg ttgctgattg gcgttgccac
     1501 ctccagtctg gccctgcacg cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga
     1561 tcaactgggt gccagcgtgg tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa
     1621 agcggcggtg cacaatcttc tcgcgcaacg cgtcagtggg ctgatcatta actatccgct
     1681 ggatgaccag gatgccattg ctgtggaagc tgcctgcact aatgttccgg cgttatttct
     1741 tgatgtctct gaccagacac ccatcaacag tattattttc tcccatgaag acggtacgcg
     1801 actgggcgtg gagcatctgg tcgcattggg tcaccagcaa atcgcgctgt tagcgggccc
     1861 attaagttct gtctcggcgc gtctgcgtct ggctggctgg cataaatatc tcactcgcaa
     1921 tcaaattcag ccgatagcgg aacgggaagg cgactggagt gccatgtccg gttttcaaca
     1981 aaccatgcaa atgctgaatg agggcatcgt tcccactgcg atgctggttg ccaacgatca
     2041 gatggcgctg ggcgcaatgc gcgccattac cgagtccggg ctgcgcgttg gtgcggatat
     2101 ctcggtagtg ggatacgacg ataccgaaga cagctcatgt tatatcccgc cgttaaccac
     2161 catcaaacag gattttcgcc tgctggggca aaccagcgtg gaccgcttgc tgcaactctc
     2221 tcagggccag gcggtgaagg gcaatcagct gttgcccgtc tcactggtga aaagaaaaac
     2281 caccctggcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     2341 gctggcacga caggtttccc gactggaaag cgggcagcca aaaaagaaga gaaaggtcta
     2401 atag
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