LOCUS       BBa_K511906             1279 bp    DNA     linear       22-Dec-2024
DEFINITION  Inducible CI434-VP16 Transactivator Generator (TRE-Tight-CI434-VP16
            ) MammoBlock Device
ACCESSION   BBa_K511906
VERSION     BBa_K511906.1  
FEATURES             Location/Qualifiers
     misc_feature    46..63
                     /label=TetO Site1
     misc_feature    81..98
                     /label=TetO Site2
     misc_feature    117..132
                     /label=TetO Site3
     misc_feature    153..170
                     /label=TetO Site4
     misc_feature    188..205
                     /label=TetO Site5
     misc_feature    224..241
                     /label=TetO Site6
     promoter        1..330
                     /label=BBa_K415506
     misc_feature    332..352
                     /label=attB1
     misc_feature    331..352
                     /label=BBa_J85600
     misc_feature    353..1015
                     /label=CI434 DNA-Binding Domain
     CDS             1016..1273
                     /label=VP16 Transactivation Domain
     misc_feature    1250..1273
                     /label=Nuclear Localization Sequence
     CDS             353..1279
                     /label=BBa_K511200
ORIGIN
        1 gctccgaatt cgcccttcag gtccgaggtt ctagacgagt ttactcccta tcagtgatag
       61 agaacgatgt cgagtttact ccctatcagt gatagagaac gtatgtcgag tttactccct
      121 atcagtgata gagaacgtat gtcgagttta ctccctatca gtgatagaga acgtatgtcg
      181 agtttatccc tatcagtgat agagaacgta tgtcgagttt actccctatc agtgatagag
      241 aacgtatgtc gaggtaggcg tgtacggtgg gaggcctata taagcagagc tcgtttagtg
      301 aaccgtcaga tcgcaaaggg cgaattcgac ccaagtttgt acaaaaaagc agatgagcat
      361 cagctccagg gtgaaatcca agcgcattca gctggggctg aaccaggccg agctggctca
      421 gaaagtggga accacacagc agagcatcga gcagctggaa aatggcaaaa ctaagcggcc
      481 cagattcctg cctgaactgg cctccgctct gggagtgtct gtggactggc tgctgaacgg
      541 cacctctgat agtaatgtgc ggtttgtggg gcacgtggag ccaaagggaa agtaccccct
      601 gattagtatg gtgagagctg gctcatggtg cgaggcctgt gaaccatacg acatcaaaga
      661 cattgatgag tggtatgaca gtgatgtgaa cctgctgggg aatggattct ggctgaaggt
      721 ggaaggcgac tcaatgacaa gccccgtggg acagtcaatc cctgagggac atatggtgct
      781 ggtggatact ggaagggaac ccgtgaacgg cagcctggtg gtggccaagc tgactgacgc
      841 caatgaggct accttcaaga aactggtcat cgatggcggg cagaaatacc tgaagggcct
      901 gaacccctcc tggcctatga caccaattaa cgggaattgc aaaatcattg gagtggtggt
      961 ggaggctcgc gtgaagtttg tggccgctaa cgacgaaaat tatgctctgg tggctgctcc
     1021 acctaccgac gtgtctctgg gcgatgagct gcacctggac ggggaagatg tggccatggc
     1081 tcatgccgac gctctggacg atttcgacct ggatatgctg ggcgacgggg attctcctgg
     1141 accaggcttt acccctcacg atagtgcccc atacggcgct ctggacatgg ccgatttcga
     1201 gtttgaacag atgtttacag acgccctggg gattgatgag tatggaggcc cacccaagaa
     1261 aaagcggaag gtgtgatga
//