BBa_K511907 1 BBa_K511907 Inducible Mnt-VP16 Transactivator Generator (TRE-Tight-Mnt-VP16) MammoBlock Device 2011-09-27T11:00:00Z 2015-05-08T01:12:31Z Consult constituent components. This MammoBlock composite device produces the Mnt-VP16 transactivator protein when induced with tTA/rtTA transactivator variants in the presence of tetracycline analogues. false false _674_ 0 6719 9 Not in stock false In order to adhere to the recombination-based MammoBlock standard, and because shipping Biosafety Level 2 mammalian expression vectors to the Registry of Standard Biological Parts is not currently possible, we are providing the sequence resulting from Gateway recombination of the constituent MammoBlock parts here without redistributing the physical DNA plasmids to the Registry. In future years, we hope to make use of improvements in the Registry's accommodations to provide our complete expression vectors. false Grant Robinson component2146417 1 BBa_J85600 component2146415 1 BBa_K415506 component2146421 1 BBa_K511201 annotation2146421 1 BBa_K511201 range2146421 1 353 898 annotation2146417 1 BBa_J85600 range2146417 1 331 352 annotation2146415 1 BBa_K415506 range2146415 1 1 330 BBa_J85600 1 BBa_J85600 attB1 recombination site 2011-09-05T11:00:00Z 2015-05-08T01:08:30Z lambda phage attB1 recombination site Used for creating mammoblock (RFC65) composite promoter-gene pairs using recombination-based cloning. false false _406_ 0 106 406 Not in stock false None false Ron Weiss annotation2125966 1 attB1 range2125966 1 2 22 BBa_K511201 1 BBa_K511201 Mnt-VP16 Transactivator MammoBlock 2011-09-24T11:00:00Z 2015-05-08T01:12:31Z This protein was synthesized from the Mnt protein produced by bacteriophage P22 and the VP16 viral transactivation domain from the herpes simplex virus. This part encodes a protein that combines the Mnt DNA-binding domain with the VP16 viral transactivation domain. This synthetic transactivator is able to moderately activate expression from minCMV-Mnt promoter variants. false false _674_ 0 6719 9 It's complicated false Not applicable. false Grant Robinson annotation2139574 1 Nuclear Localization Signal range2139574 1 517 540 annotation2139572 1 Mnt DNA-Binding Domain range2139572 1 1 282 annotation2139573 1 VP16 Transactivation Domain range2139573 1 282 540 BBa_K415506 1 BBa_K415506 pTRE-Tight L4R1 MammoBlock 2010-10-26T11:00:00Z 2015-05-08T01:12:27Z Source: modified TRE promoter. This inducible promoter can be switched on by the activity of rttA3 transcription factor. It has been optimized to contain multiple TRE binding sites; this makes the 'tight' version a significant improvement over the previously used simple TRE promoter. false true _525_ 0 6768 9 It's complicated false Increased number of TRE operator binding sites. false Laura Deming, Joy Jiao, Adrian Slusarczyk annotation2109711 1 TetO Site2 range2109711 1 81 98 annotation2109710 1 TetO Site1 range2109710 1 46 63 annotation2109713 1 TetO Site4 range2109713 1 153 170 annotation2109712 1 TetO Site3 range2109712 1 117 132 annotation2109714 1 TetO Site5 range2109714 1 188 205 annotation2109715 1 TetO Site6 range2109715 1 224 241 BBa_J85600_sequence 1 ccaagtttgtacaaaaaagcag BBa_K415506_sequence 1 gctccgaattcgcccttcaggtccgaggttctagacgagtttactccctatcagtgatagagaacgatgtcgagtttactccctatcagtgatagagaacgtatgtcgagtttactccctatcagtgatagagaacgtatgtcgagtttactccctatcagtgatagagaacgtatgtcgagtttatccctatcagtgatagagaacgtatgtcgagtttactccctatcagtgatagagaacgtatgtcgaggtaggcgtgtacggtgggaggcctatataagcagagctcgtttagtgaaccgtcagatcgcaaagggcgaattcgac BBa_K511907_sequence 1 gctccgaattcgcccttcaggtccgaggttctagacgagtttactccctatcagtgatagagaacgatgtcgagtttactccctatcagtgatagagaacgtatgtcgagtttactccctatcagtgatagagaacgtatgtcgagtttactccctatcagtgatagagaacgtatgtcgagtttatccctatcagtgatagagaacgtatgtcgagtttactccctatcagtgatagagaacgtatgtcgaggtaggcgtgtacggtgggaggcctatataagcagagctcgtttagtgaaccgtcagatcgcaaagggcgaattcgacccaagtttgtacaaaaaagcagatggctagggacgatcctcacttcaactttaggatgcccatggaggtgcgcgaaaaactgaagttccgggccgaggctaacgggagaagcatgaattccgaactgctgcagatcgtgcaggacgccctgtctaaacctagtccagtgacaggctaccggaatgacgccgagagactggctgatgagcagtccgaactggtgaagaaaatggtgtttgacactctgaaggatctgtacaagaaaaccacagccgctaacgatgaaaattatgctctggtggctgctccacctaccgacgtgagtctgggcgatgagctgcacctggacggggaagatgtggccatggctcatgccgacgctctggacgatttcgacctggatatgctgggagacggcgattcaccaggacctggcttcaccccacatgatagcgccccatacggagctctggacatggctgatttcgagtttgaacagatgttcaccgacgccctgggcattgatgagtatggcgggccacccaagaaaaagagaaaagtgtgatga BBa_K511201_sequence 1 atggctagggacgatcctcacttcaactttaggatgcccatggaggtgcgcgaaaaactgaagttccgggccgaggctaacgggagaagcatgaattccgaactgctgcagatcgtgcaggacgccctgtctaaacctagtccagtgacaggctaccggaatgacgccgagagactggctgatgagcagtccgaactggtgaagaaaatggtgtttgacactctgaaggatctgtacaagaaaaccacagccgctaacgatgaaaattatgctctggtggctgctccacctaccgacgtgagtctgggcgatgagctgcacctggacggggaagatgtggccatggctcatgccgacgctctggacgatttcgacctggatatgctgggagacggcgattcaccaggacctggcttcaccccacatgatagcgccccatacggagctctggacatggctgatttcgagtttgaacagatgttcaccgacgccctgggcattgatgagtatggcgggccacccaagaaaaagagaaaagtgtgatga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z