LOCUS       BBa_K511910             1819 bp    DNA     linear       22-Dec-2024
DEFINITION  Inducible LacI-KRAB Repressor Generator (TRE-Tight-LacI-KRAB) 
            MammoBlock Device
ACCESSION   BBa_K511910
VERSION     BBa_K511910.1  
FEATURES             Location/Qualifiers
     misc_feature    46..63
                     /label=TetO Site1
     misc_feature    81..98
                     /label=TetO Site2
     misc_feature    117..132
                     /label=TetO Site3
     misc_feature    153..170
                     /label=TetO Site4
     misc_feature    188..205
                     /label=TetO Site5
     misc_feature    224..241
                     /label=TetO Site6
     promoter        1..330
                     /label=BBa_K415506
     misc_feature    332..352
                     /label=attB1
     misc_feature    331..352
                     /label=BBa_J85600
     CDS             353..1432
                     /label=LacI
     misc_feature    1433..1816
                     /label=KRAB Domain
     CDS             353..1819
                     /label=BBa_K511205
ORIGIN
        1 gctccgaatt cgcccttcag gtccgaggtt ctagacgagt ttactcccta tcagtgatag
       61 agaacgatgt cgagtttact ccctatcagt gatagagaac gtatgtcgag tttactccct
      121 atcagtgata gagaacgtat gtcgagttta ctccctatca gtgatagaga acgtatgtcg
      181 agtttatccc tatcagtgat agagaacgta tgtcgagttt actccctatc agtgatagag
      241 aacgtatgtc gaggtaggcg tgtacggtgg gaggcctata taagcagagc tcgtttagtg
      301 aaccgtcaga tcgcaaaggg cgaattcgac ccaagtttgt acaaaaaagc agatgaaacc
      361 agtaacgtta tacgatgtcg cagagtatgc cggtgtctct tatcagaccg tttcccgcgt
      421 ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc
      481 ggagctgaat tacattccca accgcgtggc acaacaactg gcgggcaaac agtcgttgct
      541 gattggcgtt gccacctcca gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat
      601 taaatctcgc gccgatcaac tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg
      661 cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat
      721 cattaactat ccgctggatg accaggatgc cattgctgtg gaagctgcct gcactaatgt
      781 tccggcgtta tttcttgatg tctctgacca gacacccatc aacagtatta ttttctccca
      841 tgaagacggt acgcgactgg gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc
      901 gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg cgtctggctg gctggcataa
      961 atatctcact cgcaatcaaa ttcagccgat agcggaacgg gaaggcgact ggagtgccat
     1021 gtccggtttt caacaaacca tgcaaatgct gaatgagggc atcgttccca ctgcgatgct
     1081 ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg
     1141 cgttggtgcg gatatctcgg tagtgggata cgacgatacc gaagacagct catgttatat
     1201 cccgccgtta accaccatca aacaggattt tcgcctgctg gggcaaacca gcgtggaccg
     1261 cttgctgcaa ctctctcagg gccaggcggt gaagggcaat cagctgttgc ccgtctcact
     1321 ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     1381 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agccaaaaaa
     1441 gaagagaaag gtcgacggcg gtggtgcttt gtctcctcag cactctgctg tcactcaagg
     1501 aagtatcatc aagaacaagg agggcatgga tgctaagtca ctaactgcct ggtcccggac
     1561 actggtgacc ttcaaggatg tatttgtgga cttcaccagg gaggagtgga agctgctgga
     1621 cactgctcag cagatcgtgt acagaaatgt gatgctggag aactataaga acctggtttc
     1681 cttgggttat cagcttacta agccagatgt gatcctccgg ttggagaagg gagaagagcc
     1741 ctggctggtg gagagagaaa ttcaccaaga gacccatcct gattcagaga ctgcatttga
     1801 aatcaaatca tcagtttaa
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