BBa_J85600 1 BBa_J85600 attB1 recombination site 2011-09-05T11:00:00Z 2015-05-08T01:08:30Z lambda phage attB1 recombination site Used for creating mammoblock (RFC65) composite promoter-gene pairs using recombination-based cloning. false false _406_ 0 106 406 Not in stock false None false Ron Weiss annotation2125966 1 attB1 range2125966 1 2 22 BBa_K511202 1 BBa_K511202 rtTA-DD Transactivator MammoBlock 2011-09-24T11:00:00Z 2015-05-08T01:12:31Z This part consists of an Escherichia coli-derived binding domain for the tetracycline operator, the viral VP16 transactivation domain isolated from the herpes simplex virus, and an engineered human FKBP12 protein that functions as a destabilization domain. This part encodes a protein that combines the rtTA transactivator with the DD destabilization domain. The DD domain causes attached proteins to be degraded extremely quickly in mammalian cells; however, the DD domain is stable in the presence of the SHIELD molecule, allowing for tunable protein expression. Because the rtTA transactivator is dependent upon doxycycline for activation, transactivation by rtTA-DD is unique among transactivators for the dynamic range of expression it can activate. false false _674_ 0 6719 9 It's complicated false Note that the DD domain is positioned before the rtTA transactivator in this part. false Grant Robinson annotation2139588 1 Destabilization Domain range2139588 1 1 324 annotation2139589 1 rtTA Transactivation Domain range2139589 1 325 1329 BBa_K511003 1 BBa_K511003 UAS-Gal4 Promoter MammoBlock 2011-09-24T11:00:00Z 2015-05-08T01:12:31Z This part was synthesized to include the upstream activator sequence (UAS) from the Saccharomyces cerevisiae genome that binds to the Gal4 transactivator upstream of the major-late adenovirus promoter. This part encodes a promoter that is inducible by variants of the Gal4 transactivator and off otherwise. As shown in Figures 1 and 2, fluorescence from a red fluorescent protein (mKate) driven by the UAS-Gal4 promoter is very strong in the presence of constitutively produced Gal4 derivative with an attached viral VP16 transactivation domain (Gal4-VP16), and is essentially off in the absences of the Gal4-VP16 transactivator. false false _674_ 0 6719 9 It's complicated true Not applicable. false Grant Robinson annotation2139553 1 Major-Late Adenovirus Promoter range2139553 1 160 200 annotation2139552 1 Gal4-Binding Region range2139552 1 46 148 BBa_K511915 1 BBa_K511915 Inducible rtTA-DD Transactivator Generator (UAS-Gal4-rtTA-DD) MammoBlock Device 2011-09-27T11:00:00Z 2015-05-08T01:12:32Z Consult constituent components. This MammoBlock composite device produces the tetracycline-analogue-inducible and SHIELD-tunable rtTA-DD transactivator protein when induced with Gal4 transactivator variants. false false _674_ 0 6719 9 Not in stock false In order to adhere to the recombination-based MammoBlock standard, and because shipping Biosafety Level 2 mammalian expression vectors to the Registry of Standard Biological Parts is not currently possible, we are providing the sequence resulting from Gateway recombination of the constituent MammoBlock parts here without redistributing the physical DNA plasmids to the Registry. In future years, we hope to make use of improvements in the Registry's accommodations to provide our complete expression vectors. false Grant Robinson component2146510 1 BBa_J85600 component2146513 1 BBa_K511202 component2146508 1 BBa_K511003 annotation2146508 1 BBa_K511003 range2146508 1 1 220 annotation2146510 1 BBa_J85600 range2146510 1 221 242 annotation2146513 1 BBa_K511202 range2146513 1 243 1577 BBa_J85600_sequence 1 ccaagtttgtacaaaaaagcag BBa_K511202_sequence 1 atgggcgtgcaggtggagaccatcagcccaggagatggcaggacattccccaagcgcgggcagacttgcgtggtgcactacaccggcatgctggaggacgggaagaaagtggatagctccagggaccgcaacaagcccttcaagttcatgctgggaaagcaggaagtgatccgcgggtgggaggaaggagtggcccagatgagtgtgggccagcgggctaaactgactatttcacctgactacgcctatggagctaccggccacccagggatcattccccctcatgccaccctggtgttcgatgtggaactgctgaagccagagatgtcaagactggacaagagcaaagtgatcaactccgccctggagctgctgaatgaagtgggaattgagggcctgaccacaaggaagctggctcagaaactgggcgtggagcagccaactctgtattggcacgtgaagaacaaacgcgccctgctggatgccctggctattgagatgctggaccggcaccatacacatttttgccccctgaagggcgaaagctggcaggatttcctgcggaacaatgccaaatcttttagatgtgctctgctgagtcacaggaatggggccaaggtgcatagcgacacacgccccactgagaaacagtacgagaccctggaaaaccagctggccttcctgtgccagcagggcttttccctggaaaatgccctgtatgctctgtctgccgtgggacacttcacactgggctgcgtgctggaggatcaggaacatcaggtggccaaggaggaaagagagactcccactaccgattccatgccacccctgctgaggcaggccatcgaactgtttgaccaccagggcgctgagcctgccttcctgtttggcctggaactgatcatttgcgggctggaaaagcagctgaaatgcgagtctggcagtgcttacagccgggccagaacaaaaaacaattatgggtccactattgagggactgctggacctgccagacgatgacgctccagaggaagctggactggctgctccaaggctgtctttcctgcctgccggacacacccggagactgtcaaccgctcctccaacagatgtgagcctgggagacgaactgcacctggatggcgaggacgtggccatggctcatgccgatgctctggatgacttcgatctggacatgctgggggatggagactctccaggacctgggtttacccctcatgacagtgctccatacggggccctggatatggctgacttcgagtttgaacagatgtttacagatgccctgggaatcgacgagtatggcggctgatga BBa_K511915_sequence 1 gctccgaattgggacagcagagatccagtttggttaattaatagacggagtactgtcctccgagcggagtactgtcctccgactcgagcggagtactgtcctccgatcggagtactgtcctccgcgaattccggagtactgtcctccgaagacgctagcggggggctataaaagggggtgggggcgttcgtcctcactctcaattcggcccaattcgaccccaagtttgtacaaaaaagcagatgggcgtgcaggtggagaccatcagcccaggagatggcaggacattccccaagcgcgggcagacttgcgtggtgcactacaccggcatgctggaggacgggaagaaagtggatagctccagggaccgcaacaagcccttcaagttcatgctgggaaagcaggaagtgatccgcgggtgggaggaaggagtggcccagatgagtgtgggccagcgggctaaactgactatttcacctgactacgcctatggagctaccggccacccagggatcattccccctcatgccaccctggtgttcgatgtggaactgctgaagccagagatgtcaagactggacaagagcaaagtgatcaactccgccctggagctgctgaatgaagtgggaattgagggcctgaccacaaggaagctggctcagaaactgggcgtggagcagccaactctgtattggcacgtgaagaacaaacgcgccctgctggatgccctggctattgagatgctggaccggcaccatacacatttttgccccctgaagggcgaaagctggcaggatttcctgcggaacaatgccaaatcttttagatgtgctctgctgagtcacaggaatggggccaaggtgcatagcgacacacgccccactgagaaacagtacgagaccctggaaaaccagctggccttcctgtgccagcagggcttttccctggaaaatgccctgtatgctctgtctgccgtgggacacttcacactgggctgcgtgctggaggatcaggaacatcaggtggccaaggaggaaagagagactcccactaccgattccatgccacccctgctgaggcaggccatcgaactgtttgaccaccagggcgctgagcctgccttcctgtttggcctggaactgatcatttgcgggctggaaaagcagctgaaatgcgagtctggcagtgcttacagccgggccagaacaaaaaacaattatgggtccactattgagggactgctggacctgccagacgatgacgctccagaggaagctggactggctgctccaaggctgtctttcctgcctgccggacacacccggagactgtcaaccgctcctccaacagatgtgagcctgggagacgaactgcacctggatggcgaggacgtggccatggctcatgccgatgctctggatgacttcgatctggacatgctgggggatggagactctccaggacctgggtttacccctcatgacagtgctccatacggggccctggatatggctgacttcgagtttgaacagatgtttacagatgccctgggaatcgacgagtatggcggctgatga BBa_K511003_sequence 1 gctccgaattgggacagcagagatccagtttggttaattaatagacggagtactgtcctccgagcggagtactgtcctccgactcgagcggagtactgtcctccgatcggagtactgtcctccgcgaattccggagtactgtcctccgaagacgctagcggggggctataaaagggggtgggggcgttcgtcctcactctcaattcggcccaattcgacc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z