LOCUS BBa_K514000 4299 bp DNA linear 15-Nov-2024 DEFINITION C6-3OXO-HSL -> β-galactosidase activity ACCESSION BBa_K514000 VERSION BBa_K514000.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 misc_feature 81..82 /label=prefix misc_feature 254..254 /label=T misc_feature 572..572 /label=A CDS 81..830 /label=luxR misc_feature 81..836 /label=BBa_C0062 misc_feature 837..861 /label=Help:Barcodes CDS 81..836 /label=BBa_C0062 stem_loop 881..924 /label=stem_loop misc_feature 870..949 /label=BBa_B0010 terminator 870..949 /label=BBa_B0010 stem_loop 965..984 /label=T7 TE misc_feature 991..991 /label=stop polyA_site 985..998 /label=polya misc_feature 958..998 /label=BBa_B0012 terminator 958..998 /label=BBa_B0012 terminator 870..998 /label=BBa_B0015 misc_feature 1007..1026 /label=LuxR/HSL promoter 1026..1031 /label=-35 promoter 1048..1053 /label=-10 misc_feature 1059..1059 /label=start misc_feature 1007..1061 /label=BBa_R0062 promoter 1007..1061 /label=BBa_R0062 misc_feature 1..1061 /label=BBa_F2620 misc_feature 1074..1077 /label=conserved RBS 1070..1081 /label=BBa_B0034 misc_feature 4111..4111 /label=C->T CDS 1088..4162 /label=lacZ misc_feature 1088..1090 /label=START misc_feature 4160..4162 /label=STOP CDS 1088..4162 /label=BBa_I732005 stem_loop 4182..4225 /label=stem_loop misc_feature 4171..4250 /label=BBa_B0010 terminator 4171..4250 /label=BBa_B0010 stem_loop 4266..4285 /label=T7 TE misc_feature 4292..4292 /label=stop polyA_site 4286..4299 /label=polya misc_feature 4259..4299 /label=BBa_B0012 terminator 4259..4299 /label=BBa_B0012 misc_feature 1070..4299 /label=BBa_I732019 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta 121 ataaaattaa agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta 181 aaatggtaca ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat 241 ctgatatttc aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta 301 atttaataaa atatgatcct atagtagatt attctaactc caatcattca ccaattaatt 361 ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga 421 aaacatcagg tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg 481 gaatgcttag ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg 541 cgtgtatgaa cataccatta attgttcctt ctctagttga taattatcga aaaataaata 601 tagcaaataa taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg 661 catgcgaagg aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg 721 tcactttcca tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta 781 tttctaaagc aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact 841 gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc 901 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca 961 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagacct gtaggatcgt 1021 acaggtttac gcaagaaaat ggtttgttat agtcgaataa atactagaga aagaggagaa 1081 atactagatg accatgatta cggattcact ggccgtcgtt ttacaacgtc gtgactggga 1141 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg 1201 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga 1261 atggcgcttt gcctggtttc cggcaccaga agcggtgccg gaaagctggc tggagtgcga 1321 tcttcctgag gccgatactg tcgtcgtccc ctcaaactgg cagatgcacg gttacgatgc 1381 gcccatctac accaacgtga cctatcccat tacggtcaat ccgccgtttg ttcccacgga 1441 gaatccgacg ggttgttact cgctcacatt taatgttgat gaaagctggc tacaggaagg 1501 ccagacgcga attatttttg atggcgttaa ctcggcgttt catctgtggt gcaacgggcg 1561 ctgggtcggt tacggccagg acagtcgttt gccgtctgaa tttgacctga gcgcattttt 1621 acgcgccgga gaaaaccgcc tcgcggtgat ggtgctgcgc tggagtgacg gcagttatct 1681 ggaagatcag gatatgtggc ggatgagcgg cattttccgt gacgtctcgt tgctgcataa 1741 accgactaca caaatcagcg atttccatgt tgccactcgc tttaatgatg atttcagccg 1801 cgctgtactg gaggctgaag ttcagatgtg cggcgagttg cgtgactacc tacgggtaac 1861 agtttcttta tggcagggtg aaacgcaggt cgccagcggc accgcgcctt tcggcggtga 1921 aattatcgat gagcgtggtg gttatgccga tcgcgtcaca ctacgtctga acgtcgaaaa 1981 cccgaaactg tggagcgccg aaatcccgaa tctctatcgt gcggtggttg aactgcacac 2041 cgccgacggc acgctgattg aagcagaagc ctgcgatgtc ggtttccgcg aggtgcggat 2101 tgaaaatggt ctgctgctgc tgaacggcaa gccgttgctg attcgaggcg ttaaccgtca 2161 cgagcatcat cctctgcatg gtcaggtcat ggatgagcag acgatggtgc aggatatcct 2221 gctgatgaag cagaacaact ttaacgccgt gcgctgttcg cattatccga accatccgct 2281 gtggtacacg ctgtgcgacc gctacggcct gtatgtggtg gatgaagcca atattgaaac 2341 ccacggcatg gtgccaatga atcgtctgac cgatgatccg cgctggctac cggcgatgag 2401 cgaacgcgta acgcgaatgg tgcagcgcga tcgtaatcac ccgagtgtga tcatctggtc 2461 gctggggaat gaatcaggcc acggcgctaa tcacgacgcg ctgtatcgct ggatcaaatc 2521 tgtcgatcct tcccgcccgg tgcagtatga aggcggcgga gccgacacca cggccaccga 2581 tattatttgc ccgatgtacg cgcgcgtgga tgaagaccag cccttcccgg ctgtgccgaa 2641 atggtccatc aaaaaatggc tttcgctacc tggagagacg cgcccgctga tcctttgcga 2701 atacgcccac gcgatgggta acagtcttgg cggtttcgct aaatactggc aggcgtttcg 2761 tcagtatccc cgtttacagg gcggcttcgt ctgggactgg gtggatcagt cgctgattaa 2821 atatgatgaa aacggcaacc cgtggtcggc ttacggcggt gattttggcg atacgccgaa 2881 cgatcgccag ttctgtatga acggtctggt ctttgccgac cgcacgccgc atccagcgct 2941 gacggaagca aaacaccagc agcagttttt ccagttccgt ttatccgggc aaaccatcga 3001 agtgaccagc gaatacctgt tccgtcatag cgataacgag ctcctgcact ggatggtggc 3061 gctggatggt aagccgctgg caagcggtga agtgcctctg gatgtcgctc cacaaggtaa 3121 acagttgatt gaactgcctg aactaccgca gccggagagc gccgggcaac tctggctcac 3181 agtacgcgta gtgcaaccga acgcgaccgc atggtcagaa gccgggcaca tcagcgcctg 3241 gcagcagtgg cgtctggcgg aaaacctcag tgtgacgctc cccgccgcgt cccacgccat 3301 cccgcatctg accaccagcg aaatggattt ttgcatcgag ctgggtaata agcgttggca 3361 atttaaccgc cagtcaggct ttctttcaca gatgtggatt ggcgataaaa aacaactgct 3421 gacgccgctg cgcgatcagt tcacccgtgc accgctggat aacgacattg gcgtaagtga 3481 agcgacccgc attgacccta acgcctgggt cgaacgctgg aaggcggcgg gccattacca 3541 ggccgaagca gcgttgttgc agtgcacggc agatacactt gctgatgcgg tgctgattac 3601 gaccgctcac gcgtggcagc atcaggggaa aaccttattt atcagccgga aaacctaccg 3661 gattgatggt agtggtcaaa tggcgattac cgttgatgtt gaagtggcga gcgatacacc 3721 gcatccggcg cggattggcc tgaactgcca gctggcgcag gtagcagagc gggtaaactg 3781 gctcggatta gggccgcaag aaaactatcc cgaccgcctt actgccgcct gttttgaccg 3841 ctgggatctg ccattgtcag acatgtatac cccgtacgtc ttcccgagcg aaaacggtct 3901 gcgctgcggg acgcgcgaat tgaattatgg cccacaccag tggcgcggcg acttccagtt 3961 caacatcagc cgctacagtc aacagcaact gatggaaacc agccatcgcc atctgctgca 4021 cgcggaagaa ggcacatggc tgaatatcga cggtttccat atggggattg gtggcgacga 4081 ctcctggagc ccgtcagtat cggcggaatt tcagctgagc gccggtcgct accattacca 4141 gttggtctgg tgtcaaaaat aatactagag ccaggcatca aataaaacga aaggctcagt 4201 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc 4261 acactggctc accttcgggt gggcctttct gcgtttata //