LOCUS       BBa_K514001             1891 bp    DNA     linear       17-Dec-2024
DEFINITION  C6-3OXO-HSL -> C4-HSL
ACCESSION   BBa_K514001
VERSION     BBa_K514001.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    81..82
                     /label=prefix
     misc_feature    254..254
                     /label=T
     misc_feature    572..572
                     /label=A
     CDS             81..830
                     /label=luxR
     misc_feature    81..836
                     /label=BBa_C0062
     misc_feature    837..861
                     /label=Help:Barcodes
     CDS             81..836
                     /label=BBa_C0062
     stem_loop       881..924
                     /label=stem_loop
     misc_feature    870..949
                     /label=BBa_B0010
     terminator      870..949
                     /label=BBa_B0010
     stem_loop       965..984
                     /label=T7 TE
     misc_feature    991..991
                     /label=stop
     polyA_site      985..998
                     /label=polya
     misc_feature    958..998
                     /label=BBa_B0012
     terminator      958..998
                     /label=BBa_B0012
     terminator      870..998
                     /label=BBa_B0015
     misc_feature    1007..1026
                     /label=LuxR/HSL
     promoter        1026..1031
                     /label=-35
     promoter        1048..1053
                     /label=-10
     misc_feature    1059..1059
                     /label=start
     misc_feature    1007..1061
                     /label=BBa_R0062
     promoter        1007..1061
                     /label=BBa_R0062
     misc_feature    1..1061
                     /label=BBa_F2620
     misc_feature    1074..1077
                     /label=conserved
     RBS             1070..1081
                     /label=BBa_B0034
     CDS             1088..1690
                     /label=rhlL
     misc_feature    1691..1723
                     /label=LVA
     misc_feature    1730..1754
                     /label=Help:Barcodes
     CDS             1088..1754
                     /label=BBa_C0070
     stem_loop       1774..1817
                     /label=stem_loop
     misc_feature    1763..1842
                     /label=BBa_B0010
     terminator      1763..1842
                     /label=BBa_B0010
     stem_loop       1858..1877
                     /label=T7 TE
     misc_feature    1884..1884
                     /label=stop
     polyA_site      1878..1891
                     /label=polya
     misc_feature    1851..1891
                     /label=BBa_B0012
     terminator      1851..1891
                     /label=BBa_B0012
     terminator      1763..1891
                     /label=BBa_B0015
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta
      121 ataaaattaa agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta
      181 aaatggtaca ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat
      241 ctgatatttc aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta
      301 atttaataaa atatgatcct atagtagatt attctaactc caatcattca ccaattaatt
      361 ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga
      421 aaacatcagg tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg
      481 gaatgcttag ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg
      541 cgtgtatgaa cataccatta attgttcctt ctctagttga taattatcga aaaataaata
      601 tagcaaataa taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg
      661 catgcgaagg aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg
      721 tcactttcca tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta
      781 tttctaaagc aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact
      841 gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc
      901 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
      961 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagacct gtaggatcgt
     1021 acaggtttac gcaagaaaat ggtttgttat agtcgaataa atactagaga aagaggagaa
     1081 atactagatg atcgaactgc tgtccgaatc cctggaaggt ctgtccgctg ctatgatcgc
     1141 tgaactgggt cgttaccgtc accaggtttt catcgaaaaa ctgggttggg acgttgtttc
     1201 cacctcccgt gttcgtgacc aggagttcga ccagttcgac cacccgcaga cccgttacat
     1261 cgttgctatg tcccgtcagg gtatctgcgg ttgcgctcgt ctgctgccga ccaccgacgc
     1321 ttacctgctg aaagacgttt tcgcttacct gtgctccgaa accccgccgt ccgacccgtc
     1381 cgtttgggaa ctgtcccgtt acgctgcttc cgctgctgac gacccgcagc tggctatgaa
     1441 aatcttctgg tcctccctcc agtgcgcttg gtacctgggt gcttcctccg ttgttgctgt
     1501 taccaccacc gctatggaac gttacttcgt tcgtaacggt gttatcctcc agcgtctggg
     1561 tccgccgcag aaagttaaag gtgaaaccct ggttgctatc tccttcccgg cttaccagga
     1621 acgtggtctg gaaatgctgc tgcgttacca cccggaatgg ctccagggtg ttccgctgtc
     1681 catggctgtt gctgcaaacg acgaaaacta cgctttagta gcttaataac tctgatagtg
     1741 ctagtgtaga tctctactag agccaggcat caaataaaac gaaaggctca gtcgaaagac
     1801 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc
     1861 tcaccttcgg gtgggccttt ctgcgtttat a
//