BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_K515003
1
BBa_K515003
Dendra2
2011-09-06T11:00:00Z
2015-05-08T01:12:32Z
single mutation of fluorescence protein
Released HQ 2013
Dendra2 is an improved version of a green-to-red photoswitchable fluorescent protein Dendra, derived from octocoral Dendronephthya sp. [Gurskaya et al., 2006]. Dendra2 exhibits faster maturation and brighter fluorescence both before and after photoswitching than that of Dendra
true
false
_680_
0
10179
9
Discontinued
false
-
false
Atipat Patharagulpong
annotation2126153
1
Dendra2
range2126153
1
1
681
BBa_K515103
1
BBa_K515103
p(tetR) - Dendra2
2011-09-06T11:00:00Z
2015-05-08T01:12:32Z
-
Composite part between pTet and dendra2
true
false
_680_
0
10179
9
Discontinued
false
-
false
Atipat Patharagulpong
component2126163
1
BBa_R0040
component2126171
1
BBa_K515003
component2126169
1
BBa_B0034
annotation2126171
1
BBa_K515003
range2126171
1
81
761
annotation2126169
1
BBa_B0034
range2126169
1
63
74
annotation2126163
1
BBa_R0040
range2126163
1
1
54
BBa_R0040
1
p(tetR)
TetR repressible promoter
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
Lutz, R., Bujard, H., <em>Nucleic Acids Research</em> (1997) 25, 1203-1210.
Released HQ 2013
Sequence for pTet inverting regulator driven by the TetR protein.</P>
false
true
_1_
0
24
7
In stock
false
<P> <P>BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.<P>
true
June Rhee, Connie Tao, Ty Thomson, Louis Waldman
annotation1986786
1
TetR 2
range1986786
1
26
44
annotation1986787
1
-10
range1986787
1
43
48
annotation1986785
1
-35
range1986785
1
20
25
annotation1986784
1
BBa_R0040
range1986784
1
1
54
annotation1986783
1
TetR 1
range1986783
1
1
19
BBa_K515003_sequence
1
atgaacctcatcaaagaggatatgcgcgtaaaagtccacatggaaggcaacgtgaatggtcatgcgttcgtaatcgaaggtgaaggcaaagggaaaccgtacgaaggtacgcaaactgcaaacttgaccgtgaaagaaggtgcacctctgccattcagctacgacattcttaccactgcggttcactatggcaatcgtgtctttacgaaatatccggaagatattccggactacttcaaacagtcgtttccggaaggctattcatgggagcgtaccatgacctttgaggataaaggcatttgcacaattcgcagcgatattagcctggaaggggactgcttctttcagaacgttcggtttaaaggcacgaattttcctccgaatgggccggttatgcagaagaaaaccctgaaatgggaaccgtcgacagagaaactgcatgtgcgtgatggacttctggtcggtaacatcaacatggccttactgttggaaggtggaggccattatctctgtgacttcaaaaccacgtataaggcgaagaaagtggtacagctgccagatgcccactttgtggatcatcgcatcgaaattctgggcaatgactccgattacaacaaagtcaagctgtacgagcatgctgttgctcgctatagtcccttaccctctcaagtgtggtgataa
BBa_B0034_sequence
1
aaagaggagaaa
BBa_R0040_sequence
1
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
BBa_K515103_sequence
1
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgaacctgatcaaagaagacatgcgtgttaaagttcacatggaaggtaacgttaacggtcacgccttcgttatcgaaggtgaaggtaaaggtaaaccgtacgaaggtactcagaccgccaacctgaccgttaaagaaggtgcgccgctgccgttctcttacgacatcctgaccaccgcagttcactacggtaaccgtgttttcaccaaatacccggaagacatcccggactacttcaaacagtctttcccggaaggttactcttgggaacgtaccatgaccttcgaagacaaaggtatctgcaccatccgttctgacatctctctggaaggtgactgcttcttccagaacgttcgtttcaaaggtaccaacttcccgccgaacggtccggttatgcagaaaaaaaccctgaaatgggaaccgtctaccgaaaaactgcacgttcgtgacggtctgctggttggtaacatcaacatggcactgctgctggaaggtggtggtcactacctgtgcgacttcaaaaccacctacaaagccaaaaaagttgttcaactgccggacgctcacttcgttgaccaccgtatcgaaatcctgggtaacgattctgactacaacaaagttaaactgtacgaacacgcggttgcgcgttactctccgctgccgtctcaggtgtggtaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z