LOCUS       BBa_K515106             1973 bp    DNA     linear       12-May-2024
DEFINITION  J23103 promoer - RBS B0034-RFP E1010 - Holin K112805 - endolysin 
            K112806
ACCESSION   BBa_K515106
VERSION     BBa_K515106.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23103
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     CDS             62..736
                     /label=mrfp1
     misc_feature    743..767
                     /label=Help:Barcodes
     CDS             62..767
                     /label=BBa_E1010
     CDS             792..1448
                     /label=T4 holin
     CDS             776..1451
                     /label=BBa_K112805
     CDS             1476..1970
                     /label=T4 Endolysin
     CDS             1460..1973
                     /label=BBa_K112806
ORIGIN
        1 ctgatagcta gctcagtcct agggattatg ctagctacta gagaaagagg agaaatacta
       61 gatggcttcc tccgaagacg ttatcaaaga gttcatgcgt ttcaaagttc gtatggaagg
      121 ttccgttaac ggtcacgagt tcgaaatcga aggtgaaggt gaaggtcgtc cgtacgaagg
      181 tacccagacc gctaaactga aagttaccaa aggtggtccg ctgccgttcg cttgggacat
      241 cctgtccccg cagttccagt acggttccaa agcttacgtt aaacacccgg ctgacatccc
      301 ggactacctg aaactgtcct tcccggaagg tttcaaatgg gaacgtgtta tgaacttcga
      361 agacggtggt gttgttaccg ttacccagga ctcctccctg caagacggtg agttcatcta
      421 caaagttaaa ctgcgtggta ccaacttccc gtccgacggt ccggttatgc agaaaaaaac
      481 catgggttgg gaagcttcca ccgaacgtat gtacccggaa gacggtgctc tgaaaggtga
      541 aatcaaaatg cgtctgaaac tgaaagacgg tggtcactac gacgctgaag ttaaaaccac
      601 ctacatggct aaaaaaccgg ttcagctgcc gggtgcttac aaaaccgaca tcaaactgga
      661 catcacctcc cacaacgaag actacaccat cgttgaacag tacgaacgtg ctgaaggtcg
      721 tcactccacc ggtgcttaat aacgctgata gtgctagtgt agatcgctac tagagcttaa
      781 aaggagggtc tatggcagca cctagaatat cattttcgcc ctctgatatt ctatttggtg
      841 ttctcgatcg cttgttcaaa gataacgcta ccgggaaggt tcttgcttcc cgggtagctg
      901 tcgtaattct tttgtttata atggcgattg tttggtatag gggagatagt ttctttgagt
      961 actataagca atcaaagtat gaaacataca gtgaaattat tgaaaaggaa agaactgcac
     1021 gctttgaatc tgtcgccctg gaacaactcc agatagttca tatatcatct gaggcagact
     1081 ttagtgcggt gtattctttc cgccctaaaa acttaaacta ttttgttgat attatagcat
     1141 acgaaggaaa attaccttca acaataagtg aaaaatcact tggaggatat cctgttgata
     1201 aaactatgga tgaatataca gttcatttaa atggacgtca ttattattcc aactcaaaat
     1261 ttgctttttt accaactaaa aagcctactc ccgaaataaa ctacatgtac agttgtccat
     1321 attttaattt ggataatatc tatgctggaa cgataaccat gtactggtat agaaatgatc
     1381 atataagtaa tgaccgcctt gaatcaatat gtgctcaggc ggccagaata ttaggaaggg
     1441 ctaaataatt atactagaga tacttaggag gtattatgaa tatatttgaa atgttacgta
     1501 tagatgaagg tcttagactt aaaatctata aagacacaga aggctattac actattggca
     1561 tcggtcattt gcttacaaaa agtccatcac ttaatgctgc taaatctgaa ttagataaag
     1621 ctattgggcg taattgcaat ggtgtaatta caaaagatga ggctgaaaaa ctctttaatc
     1681 aggatgttga tgctgctgtt cgcggaatcc tgagaaatgc taaattaaaa ccggtttatg
     1741 attctcttga tgcggttcgt cgctgtgcat tgattaatat ggttttccaa atgggagaaa
     1801 ccggtgtggc aggatttact aactctttac gtatgcttca acaaaaacgc tgggatgaag
     1861 cagcagttaa cttagctaaa agtagatggt ataatcaaac acctaatcgc gcaaaacgag
     1921 tcattacaac gtttagaact ggcacttggg acgcgtataa aaatctataa agc
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