LOCUS       BBa_K516022              970 bp    DNA     linear       22-Dec-2024
DEFINITION  AiiA protein generator TERM+ RBS+(B0032)
ACCESSION   BBa_K516022
VERSION     BBa_K516022.1  
FEATURES             Location/Qualifiers
     RBS             1..13
                     /label=RBS-3\Weak
     RBS             7..10
                     /label=RBS
     misc_feature    1..13
                     /label=BBa_B0032
     RBS             1..13
                     /label=BBa_B0032
     misc_feature    20..22
                     /label=start
     misc_feature    803..808
                     /label=2
     misc_feature    770..802
                     /label=LVA
     CDS             20..769
                     /label=aiiA
     misc_feature    20..808
                     /label=BBa_C0060
     misc_feature    809..833
                     /label=Help:Barcodes
     CDS             20..808
                     /label=BBa_C0060
     stem_loop       853..896
                     /label=stem_loop
     misc_feature    842..921
                     /label=BBa_B0010
     terminator      842..921
                     /label=BBa_B0010
     stem_loop       937..956
                     /label=T7 TE
     misc_feature    963..963
                     /label=stop
     polyA_site      957..970
                     /label=polya
     misc_feature    930..970
                     /label=BBa_B0012
     terminator      930..970
                     /label=BBa_B0012
     terminator      842..970
                     /label=BBa_B0015
ORIGIN
        1 tcacacagga aagtactaga tgacagtaaa gaagctttat ttcgtcccag caggtcgttg
       61 tatgttggat cattcgtctg ttaatagtac attaacacca ggagaattat tagacttacc
      121 ggtttggtgt tatcttttgg agactgaaga aggacctatt ttagtagata caggtatgcc
      181 agaaagtgca gttaataatg aaggtctttt taacggtaca tttgtcgaag ggcaggtttt
      241 accgaaaatg actgaagaag atagaatcgt gaatatttta aaacgggttg gttatgagcc
      301 ggaagacctt ctttatatta ttagttctca cttgcatttt gatcatgcag gaggaaatgg
      361 cgcttttata aatacaccaa tcattgtaca gcgtgctgaa tatgaggcgg cgcagcatag
      421 cgaagaatat ttgaaagaat gtatattgcc gaatttaaac tacaaaatca ttgaaggtga
      481 ttatgaagtc gtaccaggag ttcaattatt gcatacacca ggccatactc cagggcatca
      541 atcgctatta attgagacag aaaaatccgg tcctgtatta ttaacgattg atgcatcgta
      601 tacgaaagag aattttgaaa atgaagtgcc atttgcggga tttgattcag aattagcttt
      661 atcttcaatt aaacgtttaa aagaagtggt gatgaaagag aagccgattg ttttctttgg
      721 acatgatata gagcaggaaa ggggatgtaa agtgttccct gaatatatag ctgcaaacga
      781 cgaaaactac gctttagtag cttaataacg ctgatagtgc tagtgtagat cgctactaga
      841 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt
      901 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc
      961 tgcgtttata
//