##gff-version 3
##sequence-region BBa_K516130 1 907
BBa_K516130	.	CDS	65	770	.	+	0	ID=BBa_E1010;Name=BBa_E1010
BBa_K516130	.	CDS	65	739	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K516130	.	engineered_tag	746	770	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K516130	.	promoter	1	35	.	+	0	ID=BBa_J23101;Name=BBa_J23101
BBa_K516130	.	terminator	779	907	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K516130	.	terminator	867	907	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K516130	.	polyA_site	894	907	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K516130	.	engineered_region	867	907	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K516130	.	stem_loop	874	893	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K516130	.	stop_codon	900	900	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K516130	.	terminator	779	858	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K516130	.	stem_loop	790	833	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K516130	.	engineered_region	779	858	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K516130	.	ribosome_entry_site	44	58	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_K516130	.	ribosome_entry_site	44	58	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K516130	.	ribosome_entry_site	51	55	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K516130	.	engineered_region	44	58	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
>BBa_K516130
tttacagctagctcagtcctaggtattatgctagctactagagattaaagaggagaaatactagatggcttcctccgaa
gacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtg
aaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttg
ggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaa
ctgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggact
cctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgca
gaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatg
cgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgc
cgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacg
tgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaa
taaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtca
cactggctcaccttcgggtgggcctttctgcgtttata