##gff-version 3
##sequence-region BBa_K516132 1 905
BBa_K516132	.	promoter	1	35	.	+	0	ID=BBa_J23101;Name=BBa_J23101
BBa_K516132	.	ribosome_entry_site	44	56	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_K516132	.	engineered_region	44	56	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K516132	.	ribosome_entry_site	44	56	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K516132	.	ribosome_entry_site	50	53	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K516132	.	CDS	63	768	.	+	0	ID=BBa_E1010;Name=BBa_E1010
BBa_K516132	.	engineered_tag	744	768	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K516132	.	CDS	63	737	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K516132	.	terminator	777	905	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K516132	.	terminator	777	856	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K516132	.	stem_loop	788	831	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K516132	.	engineered_region	777	856	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K516132	.	terminator	865	905	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K516132	.	stop_codon	898	898	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K516132	.	stem_loop	872	891	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K516132	.	polyA_site	892	905	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K516132	.	engineered_region	865	905	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
>BBa_K516132
tttacagctagctcagtcctaggtattatgctagctactagagtcacacaggaaagtactagatggcttcctccgaaga
cgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaa
ggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttggg
acatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaact
gtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcc
tccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcaga
aaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcg
tctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccg
ggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtg
ctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaata
aaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcaca
ctggctcaccttcgggtgggcctttctgcgtttata