##gff-version 3
##sequence-region BBa_K516220 1 1034
BBa_K516220	.	terminator	906	1034	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K516220	.	terminator	906	985	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K516220	.	stem_loop	917	960	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K516220	.	engineered_region	906	985	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K516220	.	terminator	994	1034	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K516220	.	engineered_region	994	1034	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K516220	.	stop_codon	1027	1027	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K516220	.	polyA_site	1021	1034	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K516220	.	stem_loop	1001	1020	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K516220	.	CDS	84	872	.	+	0	ID=BBa_C0060;Name=BBa_C0060
BBa_K516220	.	stop_codon	867	872	.	+	0	ID=annotation1755;Name=2;Parent=BBa_C0060
BBa_K516220	.	start_codon	84	86	.	+	0	ID=annotation1754;Name=start;Parent=BBa_C0060
BBa_K516220	.	CDS	84	833	.	+	0	ID=annotation1757;Name=aiiA;Parent=BBa_C0060
BBa_K516220	.	engineered_region	84	872	.	+	0	ID=annotation7037;Name=BBa_C0060;Parent=BBa_C0060
BBa_K516220	.	tag	834	866	.	+	0	ID=annotation1756;Name=LVA;Parent=BBa_C0060
BBa_K516220	.	engineered_tag	873	897	.	+	0	ID=annotation2213987;Name=Help:Barcodes;Parent=BBa_C0060
BBa_K516220	.	ribosome_entry_site	63	77	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_K516220	.	engineered_region	63	77	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K516220	.	ribosome_entry_site	63	77	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K516220	.	ribosome_entry_site	70	74	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K516220	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K516220	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K516220	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K516220	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K516220	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K516220	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
>BBa_K516220
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagattaaagaggagaaata
ctagatgacagtaaagaagctttatttcgtcccagcaggtcgttgtatgttggatcattcgtctgttaatagtacatta
acaccaggagaattattagacttaccggtttggtgttatcttttggagactgaagaaggacctattttagtagatacag
gtatgccagaaagtgcagttaataatgaaggtctttttaacggtacatttgtcgaagggcaggttttaccgaaaatgac
tgaagaagatagaatcgtgaatattttaaaacgggttggttatgagccggaagaccttctttatattattagttctcac
ttgcattttgatcatgcaggaggaaatggcgcttttataaatacaccaatcattgtacagcgtgctgaatatgaggcgg
cgcagcatagcgaagaatatttgaaagaatgtatattgccgaatttaaactacaaaatcattgaaggtgattatgaagt
cgtaccaggagttcaattattgcatacaccaggccatactccagggcatcaatcgctattaattgagacagaaaaatcc
ggtcctgtattattaacgattgatgcatcgtatacgaaagagaattttgaaaatgaagtgccatttgcgggatttgatt
cagaattagctttatcttcaattaaacgtttaaaagaagtggtgatgaaagagaagccgattgttttctttggacatga
tatagagcaggaaaggggatgtaaagtgttccctgaatatatagctgcaaacgacgaaaactacgctttagtagcttaa
taacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggc
ctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgc
gtttata