LOCUS       BBa_K516221             1033 bp    DNA     linear       22-May-2024
DEFINITION  AiiA protein generator with Ptet and RBS B0031
ACCESSION   BBa_K516221
VERSION     BBa_K516221.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    69..72
                     /label=conserved
     RBS             63..76
                     /label=BBa_B0031
     misc_feature    83..85
                     /label=start
     misc_feature    866..871
                     /label=2
     misc_feature    833..865
                     /label=LVA
     CDS             83..832
                     /label=aiiA
     misc_feature    83..871
                     /label=BBa_C0060
     misc_feature    872..896
                     /label=Help:Barcodes
     CDS             83..871
                     /label=BBa_C0060
     stem_loop       916..959
                     /label=stem_loop
     misc_feature    905..984
                     /label=BBa_B0010
     terminator      905..984
                     /label=BBa_B0010
     stem_loop       1000..1019
                     /label=T7 TE
     misc_feature    1026..1026
                     /label=stop
     polyA_site      1020..1033
                     /label=polya
     misc_feature    993..1033
                     /label=BBa_B0012
     terminator      993..1033
                     /label=BBa_B0012
     terminator      905..1033
                     /label=BBa_B0015
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agtcacacag gaaacctact agatgacagt aaagaagctt tatttcgtcc cagcaggtcg
      121 ttgtatgttg gatcattcgt ctgttaatag tacattaaca ccaggagaat tattagactt
      181 accggtttgg tgttatcttt tggagactga agaaggacct attttagtag atacaggtat
      241 gccagaaagt gcagttaata atgaaggtct ttttaacggt acatttgtcg aagggcaggt
      301 tttaccgaaa atgactgaag aagatagaat cgtgaatatt ttaaaacggg ttggttatga
      361 gccggaagac cttctttata ttattagttc tcacttgcat tttgatcatg caggaggaaa
      421 tggcgctttt ataaatacac caatcattgt acagcgtgct gaatatgagg cggcgcagca
      481 tagcgaagaa tatttgaaag aatgtatatt gccgaattta aactacaaaa tcattgaagg
      541 tgattatgaa gtcgtaccag gagttcaatt attgcataca ccaggccata ctccagggca
      601 tcaatcgcta ttaattgaga cagaaaaatc cggtcctgta ttattaacga ttgatgcatc
      661 gtatacgaaa gagaattttg aaaatgaagt gccatttgcg ggatttgatt cagaattagc
      721 tttatcttca attaaacgtt taaaagaagt ggtgatgaaa gagaagccga ttgttttctt
      781 tggacatgat atagagcagg aaaggggatg taaagtgttc cctgaatata tagctgcaaa
      841 cgacgaaaac tacgctttag tagcttaata acgctgatag tgctagtgta gatcgctact
      901 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
      961 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct
     1021 ttctgcgttt ata
//