##gff-version 3
##sequence-region BBa_K516221 1 1033
BBa_K516221	.	terminator	905	1033	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K516221	.	terminator	993	1033	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K516221	.	polyA_site	1020	1033	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K516221	.	engineered_region	993	1033	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K516221	.	stem_loop	1000	1019	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K516221	.	stop_codon	1026	1026	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K516221	.	terminator	905	984	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K516221	.	stem_loop	916	959	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K516221	.	engineered_region	905	984	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K516221	.	ribosome_entry_site	63	76	.	+	0	ID=BBa_B0031;Name=BBa_B0031
BBa_K516221	.	conserved_region	69	72	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
BBa_K516221	.	CDS	83	871	.	+	0	ID=BBa_C0060;Name=BBa_C0060
BBa_K516221	.	engineered_tag	872	896	.	+	0	ID=annotation2213987;Name=Help:Barcodes;Parent=BBa_C0060
BBa_K516221	.	tag	833	865	.	+	0	ID=annotation1756;Name=LVA;Parent=BBa_C0060
BBa_K516221	.	start_codon	83	85	.	+	0	ID=annotation1754;Name=start;Parent=BBa_C0060
BBa_K516221	.	stop_codon	866	871	.	+	0	ID=annotation1755;Name=2;Parent=BBa_C0060
BBa_K516221	.	CDS	83	832	.	+	0	ID=annotation1757;Name=aiiA;Parent=BBa_C0060
BBa_K516221	.	engineered_region	83	871	.	+	0	ID=annotation7037;Name=BBa_C0060;Parent=BBa_C0060
BBa_K516221	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K516221	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K516221	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K516221	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K516221	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K516221	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
>BBa_K516221
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagtcacacaggaaacctac
tagatgacagtaaagaagctttatttcgtcccagcaggtcgttgtatgttggatcattcgtctgttaatagtacattaa
caccaggagaattattagacttaccggtttggtgttatcttttggagactgaagaaggacctattttagtagatacagg
tatgccagaaagtgcagttaataatgaaggtctttttaacggtacatttgtcgaagggcaggttttaccgaaaatgact
gaagaagatagaatcgtgaatattttaaaacgggttggttatgagccggaagaccttctttatattattagttctcact
tgcattttgatcatgcaggaggaaatggcgcttttataaatacaccaatcattgtacagcgtgctgaatatgaggcggc
gcagcatagcgaagaatatttgaaagaatgtatattgccgaatttaaactacaaaatcattgaaggtgattatgaagtc
gtaccaggagttcaattattgcatacaccaggccatactccagggcatcaatcgctattaattgagacagaaaaatccg
gtcctgtattattaacgattgatgcatcgtatacgaaagagaattttgaaaatgaagtgccatttgcgggatttgattc
agaattagctttatcttcaattaaacgtttaaaagaagtggtgatgaaagagaagccgattgttttctttggacatgat
atagagcaggaaaggggatgtaaagtgttccctgaatatatagctgcaaacgacgaaaactacgctttagtagcttaat
aacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcc
tttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcg
tttata