LOCUS       BBa_K516231              925 bp    DNA     linear       27-Dec-2024
DEFINITION  PTet mRFP with RBS B0031
ACCESSION   BBa_K516231
VERSION     BBa_K516231.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    69..72
                     /label=conserved
     RBS             63..76
                     /label=BBa_B0031
     CDS             83..757
                     /label=mrfp1
     misc_feature    764..788
                     /label=Help:Barcodes
     CDS             83..788
                     /label=BBa_E1010
     stem_loop       808..851
                     /label=stem_loop
     misc_feature    797..876
                     /label=BBa_B0010
     terminator      797..876
                     /label=BBa_B0010
     stem_loop       892..911
                     /label=T7 TE
     misc_feature    918..918
                     /label=stop
     polyA_site      912..925
                     /label=polya
     misc_feature    885..925
                     /label=BBa_B0012
     terminator      885..925
                     /label=BBa_B0012
     terminator      797..925
                     /label=BBa_B0015
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agtcacacag gaaacctact agatggcttc ctccgaagac gttatcaaag agttcatgcg
      121 tttcaaagtt cgtatggaag gttccgttaa cggtcacgag ttcgaaatcg aaggtgaagg
      181 tgaaggtcgt ccgtacgaag gtacccagac cgctaaactg aaagttacca aaggtggtcc
      241 gctgccgttc gcttgggaca tcctgtcccc gcagttccag tacggttcca aagcttacgt
      301 taaacacccg gctgacatcc cggactacct gaaactgtcc ttcccggaag gtttcaaatg
      361 ggaacgtgtt atgaacttcg aagacggtgg tgttgttacc gttacccagg actcctccct
      421 gcaagacggt gagttcatct acaaagttaa actgcgtggt accaacttcc cgtccgacgg
      481 tccggttatg cagaaaaaaa ccatgggttg ggaagcttcc accgaacgta tgtacccgga
      541 agacggtgct ctgaaaggtg aaatcaaaat gcgtctgaaa ctgaaagacg gtggtcacta
      601 cgacgctgaa gttaaaacca cctacatggc taaaaaaccg gttcagctgc cgggtgctta
      661 caaaaccgac atcaaactgg acatcacctc ccacaacgaa gactacacca tcgttgaaca
      721 gtacgaacgt gctgaaggtc gtcactccac cggtgcttaa taacgctgat agtgctagtg
      781 tagatcgcta ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
      841 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct
      901 tcgggtgggc ctttctgcgt ttata
//