##gff-version 3
##sequence-region BBa_K516231 1 925
BBa_K516231	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K516231	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K516231	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K516231	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K516231	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K516231	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K516231	.	terminator	797	925	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K516231	.	terminator	797	876	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K516231	.	stem_loop	808	851	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K516231	.	engineered_region	797	876	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K516231	.	terminator	885	925	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K516231	.	stem_loop	892	911	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K516231	.	stop_codon	918	918	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K516231	.	polyA_site	912	925	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K516231	.	engineered_region	885	925	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K516231	.	CDS	83	788	.	+	0	ID=BBa_E1010;Name=BBa_E1010
BBa_K516231	.	engineered_tag	764	788	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K516231	.	CDS	83	757	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K516231	.	ribosome_entry_site	63	76	.	+	0	ID=BBa_B0031;Name=BBa_B0031
BBa_K516231	.	conserved_region	69	72	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
>BBa_K516231
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagtcacacaggaaacctac
tagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacg
agttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtgg
tccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgac
atcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttg
ttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtc
cgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctg
aaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggcta
aaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccat
cgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgcta
ctagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaa
cgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata