LOCUS       BBa_K516330             1841 bp    DNA     linear       23-May-2024
DEFINITION  mRFP producer controlled by 3OC6-HSL with RBS B0030
ACCESSION   BBa_K516330
VERSION     BBa_K516330.1  
FEATURES             Location/Qualifiers
     promoter        38..43
                     /label=-10
     promoter        15..20
                     /label=-35
     misc_feature    25..41
                     /label=OR1
     misc_feature    1..17
                     /label=OR2
     misc_feature    1..49
                     /label=BBa_R0051
     promoter        1..49
                     /label=BBa_R0051
     RBS             58..72
                     /label=RBS-1\Strong
     RBS             65..69
                     /label=RBS
     misc_feature    58..72
                     /label=BBa_B0030
     RBS             58..72
                     /label=BBa_B0030
     misc_feature    79..80
                     /label=prefix
     misc_feature    252..252
                     /label=T
     misc_feature    570..570
                     /label=A
     CDS             79..828
                     /label=luxR
     misc_feature    79..834
                     /label=BBa_C0062
     misc_feature    835..859
                     /label=Help:Barcodes
     CDS             79..834
                     /label=BBa_C0062
     misc_feature    868..906
                     /label=B1006
     stem_loop       877..898
                     /label=modified thr terminator
     polyA_site      899..906
                     /label=PolyA
     polyA_site      868..876
                     /label=PolyA
     terminator      868..906
                     /label=BBa_B1006
     misc_feature    915..934
                     /label=LuxR/HSL
     promoter        934..939
                     /label=-35
     promoter        956..961
                     /label=-10
     misc_feature    967..967
                     /label=start
     misc_feature    915..969
                     /label=BBa_R0062
     promoter        915..969
                     /label=BBa_R0062
     RBS             978..992
                     /label=RBS-1\Strong
     RBS             985..989
                     /label=RBS
     misc_feature    978..992
                     /label=BBa_B0030
     RBS             978..992
                     /label=BBa_B0030
     CDS             999..1673
                     /label=mrfp1
     misc_feature    1680..1704
                     /label=Help:Barcodes
     CDS             999..1704
                     /label=BBa_E1010
     stem_loop       1724..1767
                     /label=stem_loop
     misc_feature    1713..1792
                     /label=BBa_B0010
     terminator      1713..1792
                     /label=BBa_B0010
     stem_loop       1808..1827
                     /label=T7 TE
     misc_feature    1834..1834
                     /label=stop
     polyA_site      1828..1841
                     /label=polya
     misc_feature    1801..1841
                     /label=BBa_B0012
     terminator      1801..1841
                     /label=BBa_B0012
     terminator      1713..1841
                     /label=BBa_B0015
ORIGIN
        1 taacaccgtg cgtgttgact attttacctc tggcggtgat aatggttgct actagagatt
       61 aaagaggaga aatactagat gaaaaacata aatgccgacg acacatacag aataattaat
      121 aaaattaaag cttgtagaag caataatgat attaatcaat gcttatctga tatgactaaa
      181 atggtacatt gtgaatatta tttactcgcg atcatttatc ctcattctat ggttaaatct
      241 gatatttcaa tcctagataa ttaccctaaa aaatggaggc aatattatga tgacgctaat
      301 ttaataaaat atgatcctat agtagattat tctaactcca atcattcacc aattaattgg
      361 aatatatttg aaaacaatgc tgtaaataaa aaatctccaa atgtaattaa agaagcgaaa
      421 acatcaggtc ttatcactgg gtttagtttc cctattcata cggctaacaa tggcttcgga
      481 atgcttagtt ttgcacattc agaaaaagac aactatatag atagtttatt tttacatgcg
      541 tgtatgaaca taccattaat tgttccttct ctagttgata attatcgaaa aataaatata
      601 gcaaataata aatcaaacaa cgatttaacc aaaagagaaa aagaatgttt agcgtgggca
      661 tgcgaaggaa aaagctcttg ggatatttca aaaatattag gttgcagtga gcgtactgtc
      721 actttccatt taaccaatgc gcaaatgaaa ctcaatacaa caaaccgctg ccaaagtatt
      781 tctaaagcaa ttttaacagg agcaattgat tgcccatact ttaaaaatta ataacactga
      841 tagtgctagt gtagatcact actagagaaa aaaaaacccc gcccctgaca gggcggggtt
      901 tttttttact agagacctgt aggatcgtac aggtttacgc aagaaaatgg tttgttatag
      961 tcgaataaat actagagatt aaagaggaga aatactagat ggcttcctcc gaagacgtta
     1021 tcaaagagtt catgcgtttc aaagttcgta tggaaggttc cgttaacggt cacgagttcg
     1081 aaatcgaagg tgaaggtgaa ggtcgtccgt acgaaggtac ccagaccgct aaactgaaag
     1141 ttaccaaagg tggtccgctg ccgttcgctt gggacatcct gtccccgcag ttccagtacg
     1201 gttccaaagc ttacgttaaa cacccggctg acatcccgga ctacctgaaa ctgtccttcc
     1261 cggaaggttt caaatgggaa cgtgttatga acttcgaaga cggtggtgtt gttaccgtta
     1321 cccaggactc ctccctgcaa gacggtgagt tcatctacaa agttaaactg cgtggtacca
     1381 acttcccgtc cgacggtccg gttatgcaga aaaaaaccat gggttgggaa gcttccaccg
     1441 aacgtatgta cccggaagac ggtgctctga aaggtgaaat caaaatgcgt ctgaaactga
     1501 aagacggtgg tcactacgac gctgaagtta aaaccaccta catggctaaa aaaccggttc
     1561 agctgccggg tgcttacaaa accgacatca aactggacat cacctcccac aacgaagact
     1621 acaccatcgt tgaacagtac gaacgtgctg aaggtcgtca ctccaccggt gcttaataac
     1681 gctgatagtg ctagtgtaga tcgctactag agccaggcat caaataaaac gaaaggctca
     1741 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag
     1801 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a
//