##gff-version 3
##sequence-region BBa_K516331 1 1840
BBa_K516331	.	terminator	868	906	.	+	0	ID=BBa_B1006;Name=BBa_B1006
BBa_K516331	.	polyA_site	868	876	.	+	0	ID=annotation1898431;Name=PolyA;Parent=BBa_B1006
BBa_K516331	.	polyA_site	899	906	.	+	0	ID=annotation1898430;Name=PolyA;Parent=BBa_B1006
BBa_K516331	.	engineered_region	868	906	.	+	0	ID=annotation1898428;Name=B1006;Parent=BBa_B1006
BBa_K516331	.	stem_loop	877	898	.	+	0	ID=annotation1898429;Name=modified thr terminator;Parent=BBa_B1006
BBa_K516331	.	promoter	915	969	.	+	0	ID=BBa_R0062;Name=BBa_R0062
BBa_K516331	.	promoter	934	939	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K516331	.	non_covalent_binding_site	915	934	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K516331	.	engineered_region	915	969	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K516331	.	start_codon	967	967	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K516331	.	promoter	956	961	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K516331	.	ribosome_entry_site	58	72	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_K516331	.	ribosome_entry_site	65	69	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K516331	.	engineered_region	58	72	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K516331	.	ribosome_entry_site	58	72	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K516331	.	CDS	998	1703	.	+	0	ID=BBa_E1010;Name=BBa_E1010
BBa_K516331	.	CDS	998	1672	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K516331	.	engineered_tag	1679	1703	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K516331	.	ribosome_entry_site	978	991	.	+	0	ID=BBa_B0031;Name=BBa_B0031
BBa_K516331	.	conserved_region	984	987	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
BBa_K516331	.	terminator	1712	1840	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K516331	.	terminator	1712	1791	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K516331	.	stem_loop	1723	1766	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K516331	.	engineered_region	1712	1791	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K516331	.	terminator	1800	1840	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K516331	.	stem_loop	1807	1826	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K516331	.	stop_codon	1833	1833	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K516331	.	engineered_region	1800	1840	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K516331	.	polyA_site	1827	1840	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K516331	.	CDS	79	834	.	+	0	ID=BBa_C0062;Name=BBa_C0062
BBa_K516331	.	CDS	79	828	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K516331	.	engineered_region	79	834	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K516331	.	engineered_region	79	80	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K516331	.	point_mutation	252	252	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K516331	.	engineered_tag	835	859	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K516331	.	point_mutation	570	570	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K516331	.	promoter	1	49	.	+	0	ID=BBa_R0051;Name=BBa_R0051
BBa_K516331	.	promoter	15	20	.	+	0	ID=annotation2023;Name=-35;Parent=BBa_R0051
BBa_K516331	.	non_covalent_binding_site	25	41	.	+	0	ID=annotation2024;Name=OR1;Parent=BBa_R0051
BBa_K516331	.	non_covalent_binding_site	1	17	.	+	0	ID=annotation2025;Name=OR2;Parent=BBa_R0051
BBa_K516331	.	engineered_region	1	49	.	+	0	ID=annotation7067;Name=BBa_R0051;Parent=BBa_R0051
BBa_K516331	.	promoter	38	43	.	+	0	ID=annotation2022;Name=-10;Parent=BBa_R0051
>BBa_K516331
taacaccgtgcgtgttgactattttacctctggcggtgataatggttgctactagagattaaagaggagaaatactaga
tgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatca
atgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaa
tctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatc
ctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatc
tccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggc
ttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacatac
cattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaa
aagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgag
cgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaa
ttttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagaa
aaaaaaaccccgcccctgacagggcggggtttttttttactagagacctgtaggatcgtacaggtttacgcaagaaaat
ggtttgttatagtcgaataaatactagagtcacacaggaaacctactagatggcttcctccgaagacgttatcaaagag
ttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtc
cgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtcccc
gcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaa
ggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacg
gtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatggg
ttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaa
gacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaa
ccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtca
ctccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctc
agtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttc
gggtgggcctttctgcgtttata