LOCUS       BBa_K518002             2884 bp    DNA     linear       25-Nov-2024
DEFINITION  Firefly-renilla dual luciferase assay kit
ACCESSION   BBa_K518002
VERSION     BBa_K518002.1  
FEATURES             Location/Qualifiers
     RBS             1..13
                     /label=RBS-3\Weak
     RBS             7..10
                     /label=RBS
     misc_feature    1..13
                     /label=BBa_B0032
     RBS             1..13
                     /label=BBa_B0032
     CDS             20..1672
                     /label=BBa_I712019
     stem_loop       1688..1707
                     /label=T7 TE
     misc_feature    1714..1714
                     /label=stop
     polyA_site      1708..1721
                     /label=polya
     misc_feature    1681..1721
                     /label=BBa_B0012
     terminator      1681..1721
                     /label=BBa_B0012
     stem_loop       1742..1764
                     /label=stem_loop
     misc_feature    1730..1775
                     /label=BBa_B0011
     terminator      1730..1775
                     /label=BBa_B0011
     terminator      1681..1775
                     /label=BBa_B0014
     promoter        1784..1818
                     /label=BBa_J23118
     RBS             1827..1839
                     /label=RBS-3\Weak
     RBS             1833..1836
                     /label=RBS
     misc_feature    1827..1839
                     /label=BBa_B0032
     RBS             1827..1839
                     /label=BBa_B0032
     misc_feature    1846..2781
                     /label=Rluc
     CDS             1846..2781
                     /label=Rluc
     misc_feature    1846..2781
                     /label=BBa_J52008
     stem_loop       2797..2816
                     /label=T7 TE
     misc_feature    2823..2823
                     /label=stop
     polyA_site      2817..2830
                     /label=polya
     misc_feature    2790..2830
                     /label=BBa_B0012
     terminator      2790..2830
                     /label=BBa_B0012
     stem_loop       2851..2873
                     /label=stem_loop
     misc_feature    2839..2884
                     /label=BBa_B0011
     terminator      2839..2884
                     /label=BBa_B0011
     terminator      2790..2884
                     /label=BBa_B0014
ORIGIN
        1 tcacacagga aagtactaga tggaagacgc caaaaacata aagaaaggcc cggcgccatt
       61 ctatccgctg gaagatggaa ccgctggaga gcaactgcat aaggctatga agagatacgc
      121 cctggttcct ggaacaattg cttttacaga tgcacatatc gaggtggaca tcacttacgc
      181 tgagtacttc gaaatgtccg ttcggttggc agaagctatg aaacgatatg ggctgaatac
      241 aaatcacaga atcgtcgtat gcagtgaaaa ctctcttcaa ttctttatgc cggtgttggg
      301 cgcgttattt atcggagttg cagttgcgcc cgcgaacgac atttataatg aacgtgaatt
      361 gctcaacagt atgggcattt cgcagcctac cgtggtgttc gtttccaaaa aggggttgca
      421 aaaaattttg aacgtgcaaa aaaagctccc aatcatccaa aaaattatta tcatggattc
      481 taaaacggat taccagggat ttcagtcgat gtacacgttc gtcacatctc atctacctcc
      541 cggttttaat gaatacgatt ttgtgccaga gtccttcgat agggacaaga caattgcact
      601 gatcatgaac tcctctggat ctactggtct gcctaaaggt gtcgctctgc ctcatagaac
      661 tgcctgcgtg agattctcgc atgccagaga tcctattttt ggcaatcaaa tcattccgga
      721 tactgcgatt ttaagtgttg ttccattcca tcacggtttt ggaatgttta ctacactcgg
      781 atatttgata tgtggatttc gagtcgtctt aatgtataga tttgaagaag agctgtttct
      841 gaggagcctt caggattaca agattcaaag tgcgctgctg gtgccaaccc tattctcctt
      901 cttcgccaaa agcactctga ttgacaaata cgatttatct aatttacacg aaattgcttc
      961 tggtggcgct cccctctcta aggaagtcgg ggaagcggtt gccaagaggt tccatctgcc
     1021 aggtatcagg caaggatatg ggctcactga gactacatca gctattctga ttacacccga
     1081 gggggatgat aaaccgggcg cggtcggtaa agttgttcca ttttttgaag cgaaggttgt
     1141 ggatctggat accgggaaaa cgctgggcgt taatcaaaga ggcgaactgt gtgtgagagg
     1201 tcctatgatt atgtccggtt atgtaaacaa tccggaagcg accaacgcct tgattgacaa
     1261 ggatggatgg ctacattctg gagacatagc ttactgggac gaagacgaac acttcttcat
     1321 cgttgaccgc ctgaagtctc tgattaagta caaaggctat caggtggctc ccgctgaatt
     1381 ggaatccatc ttgctccaac accccaacat cttcgacgca ggtgtcgcag gtcttcccga
     1441 cgatgacgcc ggtgaacttc ccgccgccgt tgttgttttg gagcacggaa agacgatgac
     1501 ggaaaaagag atcgtggatt acgtcgccag tcaagtaaca accgcgaaaa agttgcgcgg
     1561 aggagttgtg tttgtggacg aagtaccgaa aggtcttacc ggaaaactcg acgcaagaaa
     1621 aatcagagag atcctcataa aggccaagaa gggcggaaag atcgccgtgt aatactagag
     1681 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atactagaga gagaatataa
     1741 aaagccagat tattaatccg gcttttttat tattttacta gagttgacgg ctagctcagt
     1801 cctaggtatt gtgctagcta ctagagtcac acaggaaagt actagatggc ttccaaggtg
     1861 tacgaccccg agcaacgcaa acgcatgatc actgggcctc agtggtgggc tcgctgcaag
     1921 caaatgaacg tgctggactc cttcatcaac tactatgatt ccgagaagca cgccgagaac
     1981 gccgtgattt ttctgcatgg taacgctgcc tccagctacc tgtggaggca cgtcgtgcct
     2041 cacatcgagc ccgtggctag atgcatcatc cctgatctga tcggaatggg taagtccggc
     2101 aagagcggga atggctcata tcgcctcctg gatcactaca agtacctcac cgcttggttc
     2161 gagctgctga accttccaaa gaaaatcatc tttgtgggcc acgactgggg ggcttgtctg
     2221 gcctttcact actcctacga gcaccaagac aagatcaagg ccatcgtcca tgctgagagt
     2281 gtcgtggacg tgatcgagtc ctgggacgag tggcctgaca tcgaggagga tatcgccctg
     2341 atcaagagcg aagagggcga gaaaatggtg cttgagaata acttcttcgt cgagaccatg
     2401 ctcccaagca agatcatgcg gaaactggag cctgaggagt tcgctgccta cctggagcca
     2461 ttcaaggaga agggcgaggt tagacggcct accctctcct ggcctcgcga gatccctctc
     2521 gttaagggag gcaagcccga cgtcgtccag attgtccgca actacaacgc ctaccttcgg
     2581 gccagcgacg atctgcctaa gatgttcatc gagtccgacc ctgggttctt ttccaacgct
     2641 attgtcgagg gagctaagaa gttccctaac accgagttcg tgaaggtgaa gggcctccac
     2701 ttcagccagg aggacgctcc agatgaaatg ggtaagtaca tcaagagctt cgtggagcgc
     2761 gtgctgaaga acgagcagta atactagagt cacactggct caccttcggg tgggcctttc
     2821 tgcgtttata tactagagag agaatataaa aagccagatt attaatccgg cttttttatt
     2881 attt
//